Human CCL2/MCP-1 Quantikine ELISA Kit | hCCL2/MCP-1 QKit
掲載日情報：2016/08/18 現在Webページ番号：111060
R&D Systems製のHuman CCL2/MCP-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月29日 15時35分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
MCP-1, Human, ELISA Kit, Quantikine (96well) |
|
342 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2024年04月29日 15時35分現在]
MCP-1, Human, ELISA Kit, Quantikine (96well)
文献数： 342
- 商品コード：DCP00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：31.20 - 2000 pg/mL，感度：10 pg/mL，測定試料：Cell Culture Supernates (200 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC01-1，測定時間：3.5 hours or 4.5 hours，測定因子：CCL2/JE/MCP-1 別名：C-C motif chemokine ligand 2 Genbank No： 6347 |
||||||
|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | 医薬用外毒物 | ||||||
| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
ニュース2021年5月1日号 p.7
|
||||||
| 製品記事 |
CCL2/MCP-1 Quantikine/DuoSet ELISA Kit 新型コロナウイルス感染に起因するサイトカインストームのモニタリング用製品 |
||||||
| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
||||||
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours or 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (200 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)
- Sensitivity
- 10 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human MCP-1
- Interference
- No significant interference observed with available related molecules.
追加しました。
Product Summary
The Quantikine Human MCP-1 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MCP-1 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human MCP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MCP-1. Results obtained using natural human MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MCP-1.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 76.7 | 364 | 1,121 | 74.2 | 352 | 1,076 |
| Standard Deviation | 6 | 17.1 | 54.4 | 5 | 20.5 | 49.4 |
| CV% | 7.8 | 4.7 | 4.9 | 6.7 | 5.8 | 4.6 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 78.2 | 360 | 1,079 | 76 | 360 | 1,086 |
| Standard Deviation | 3.3 | 17.7 | 63.5 | 4.5 | 17.2 | 48.5 |
| CV% | 4.2 | 4.9 | 5.9 | 5.9 | 4.8 | 4.5 |
追加しました。
Recovery
The recovery of MCP-1 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 103 | 92-113 |
| Heparin Plasma (n=5) | 102 | 94-114 |
| Citrate Plasma (n=5) | 100 | 94-107 |
| EDTA Plasma (n=5) | 96 | 92-102 |
| Urine (n=5) | 92 | 85-100 |
| Cell Culture Media (n=5) | 96 | 88-107 |
追加しました。
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of MCP-1 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
追加しました。
Additional Infomation
- Molecule Information
- CCL2/JE/MCP-1
- Aliases
- MCAF; MCP-1
- Entrez Gene IDs
- 6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
- Background
- CCL2/MCP-1/JE
- MCP-1, also known as monocyte chemotactic and activating factor (MCAF), was initially purified based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene.
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Hypoxia potentiates glioma-mediated immunosuppression.
Fokt I, Fuller G, Heimberger AB, Kong LY, Melillo G, Priebe W, Wang Y, Wei J, Wu A
PLoS ONE 2012 6:e16195
Species: Human
Sample Type: Cell Culture Supernates
Alveolar fibrocyte percentage is an independent predictor of poor outcome in patients with acute lung injury.
Bouadma L, Crestani B, Dehoux M, Elbim C, Garnier M, Gelly J, Lasocki S, Lecon V, Mentre F, Nardelli L, Philip I, Piednoir P, Quesnel C, Soler P
Crit. Care Med. 2012 40:21-8
Species: Human
Sample Type: BALF
A composite urine biomarker reflects interstitial inflammation in lupus nephritis kidney biopsies.
Nadasdy T, Nagaraja HN, Zhang X
Kidney Int. 2012 81:401-6
Species: Human
Sample Type: Urine
Exenatide as a weight-loss therapy in extreme pediatric obesity: a randomized, controlled pilot study.
Abuzzahab MJ, Billington CJ, Deering MM, Fitch AK, Fox CK, Gandrud LM, Kelly AS, Metzig AM, Moran A, Nathan BM, Rudser KD, Schwartz BL, Schwarzenberg SJ
Obesity (Silver Spring) 2012 20:364-70
Species: Human
Sample Type: Plasma
Anti-inflammatory effects of methoxyphenolic compounds on human airway cells.
Houser KR, Ishmael FT, Johnson DK
J Inflamm (Lond) 2012 9:6
Species: Human
Sample Type: Cell Culture Supernates
Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function.
Castleton AZ, Dey A, Elham M, Fielding AK, Ghorani E, Patel B, Rai L, Zhang Y
J. Immunol. 2012 188:1002-10
Prevalence of Chronic Kidney Disease and Progression of Disease Over Time among Patients Enrolled in the Houston West Nile Virus Cohort.
Hause AM, Nolan MS, Podoll AS
PLoS ONE 2012 7:e40374
Species: Human
Sample Type: Urine
Increased levels of inflammatory cytokines and endothelin-1 in alveolar macrophages from patients with chronic heart failure.
??Ie E, Andreassen AK, Aukrust P, Dahl CP, Edvardsen T, Gude E, Gullestad L, Holm T, Kongerud J, Sikkeland LI, Ueland T, Yndestad A
PLoS ONE 2012 7:e36815
Species:
Sample Type: Plasma
Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue Fever.
Alves AM, Avila CA, Barbosa LD, da Cunha RV, Damasco PV, de Azeredo EL, de Souza LA, de Souza LJ, de-Oliveira-Pinto LM, Kubelka CF, Marinho CF, Motta-Castro AR, Paes MV, Povoa TF
PLoS ONE 2012 7:e38527
Species: Human
Sample Type: Plasma
Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
Andersson M, Godaly G, Hallgren O, Lutay N, Svensson M, Westergren-Thorsson G
Innate Immun 2012 18:531-40
Species: Human
Sample Type: Cell Culture Supernates
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。