Human COMP Quantikine ELISA Kit | hCOMP QKit
掲載日情報：2016/08/18 現在Webページ番号：111059
R&D Systems製のHuman COMPを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月20日 16時15分現在]
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COMP, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月20日 16時15分現在]
COMP, Human, ELISA Kit, Quantikine (96well)
文献数： 7
- 商品コード：DCMP0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.16 - 10 ng/mL，感度：0.036 ng/mL，測定試料：Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC66，測定時間：4.5 hours，測定因子：COMP/Thrombospondin-5 別名：cartilage oligomeric matrix protein Genbank No： 1311 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Human COMP Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.036 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
- Specificity
- Natural and recombinant human COMP
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human COMP immunoassay is a 4.5 hour solid phase ELISA designed to measure human COMP in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human COMP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human COMP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring COMP.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.9 | 2.92 | 6.11 | 1.05 | 3.07 | 6.26 |
| Standard Deviation | 0.028 | 0.112 | 0.225 | 0.045 | 0.146 | 0.3 |
| CV% | 2.1 | 3.8 | 3.7 | 4.3 | 4.8 | 4.8 |
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Recovery
The recovery of COMP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 102 | 86-114 |
| Heparin Plasma (n=4) | 99 | 88-112 |
| Cell Lysates (n=1) | 102 | 98-108 |
| EDTA Plasma (n=4) | 103 | 86-114 |
| Cell Culture Media (n=5) | 103 | 95-111 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human COMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- COMP/Thrombospondin-5
- Aliases
- EDM1; EPD1; MED; PSACH; Thrombospondin-5
- Entrez Gene IDs
- 1311 (Human); 12845 (Mouse)
- Background
- COMP
- COMP/Thrombospondin-5 is a multidomain calcium binding matricellular protein. Thrombospondin-1 and -2 form homotrimers, whereas Thrombospondin-3, -4, and COMP form homopentamers. COMP mediates chondrocyte-matrix adhesion via Integrin binding. COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas. Mutations in COMP are associated with skeletal dysplasias and disorders of calcium metabolism.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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