Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit | hCD40L Qkit
掲載日情報：2016/08/18 現在Webページ番号：111052
R&D Systems製のHuman CD40 Ligand/TNFSF5を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月24日 00時00分現在]
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CD40 Ligand, Soluble, Human, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2024年04月24日 00時00分現在]
CD40 Ligand, Soluble, Human, ELISA Kit, Quantikine (96 well)
文献数： 68
- 商品コード：DCDL40
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：62.50 - 4000 pg/mL，感度：10.1 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (20 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC22，測定時間：4.5 hours，測定因子：CD40 Ligand/TNFSF5 別名：CD154 Genbank No： 959 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
ニュース2022年11月15日号 p.6
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| 製品記事 |
Immune Checkpoint Protein ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (20 µL), Platelet-poor EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)
- Sensitivity
- 10.1 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant human CD40 Ligand
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human CD40 Ligand Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CD40 Ligand in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human CD40 Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD40 Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD40 Ligand.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 430 | 1,212 | 2,638 | 437 | 1,205 | 2,612 |
| Standard Deviation | 21.8 | 54.4 | 143 | 27.9 | 72.7 | 162 |
| CV% | 5.1 | 4.5 | 5.4 | 6.4 | 6 | 6.2 |
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Recovery
The recovery of CD40 Ligand spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Platelet-poor EDTA Plasma (n=5) | 98 | 86-107 |
| Platelet-poor Heparin Plasma (n=5) | 94 | 86-113 |
| Cell Culture Media (n=4) | 102 | 90-114 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CD40 Ligand were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CD40 Ligand/TNFSF5
- Aliases
- CD154; CD40LG; gp39; TNFSF5; TRAP
- Entrez Gene IDs
- 959 (Human); 21947 (Mouse); 84349 (Rat)
- Background
- CD40 Ligand/TNFSF5
- CD40 Ligand/TNFSF5 in Human Tonsils.CD40 ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 260 amino acid type II transmembrane glycoprotein belonging to the TNF family. Murine CD40L consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane domain, and 214 aa extracellular domain bearing a single glycosylation site. CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including NK cells, mast cells, basophils and eosinophils. Murine CD40L shares 78% amino acid sequence identity with human CD40L. Native bioactive soluble CD40L exists. Soluble human trimeric CD40L secreted by stimulated T cells has been shown to be generated by proteolysis in the microsomes. Both membrane bound and soluble CD40L induce similar effects on B cells.The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells, and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the soluble trimeric form of CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. The genetic defect in the hyper-IgM syndrome is due to point mutations or deletions of the gene encoding the CD40L, which prevent CD40L from interacting with CD40. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching, Ig secretion, and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Elderly trauma patients have high circulating noradrenaline levels but attenuated release of adrenaline, platelets, and leukocytes in response to increasing injury severity.
Johansson PI, Larsen CF, Ostrowski SR, Perner A, Sorensen AM, Wanscher M, Welling KL
Crit. Care Med. 2012 40:1844-50
Species: Human
Sample Type: Plasma
Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
J. Thromb. Haemost. 2012 10:1616-23
Prospective study of circulating soluble CD40 ligand concentrations and the incidence of cardiovascular disease in a nested prospective case-control study of older men and women.
Ebrahim S, Jefferis BJ, Lawlor DA, Lowe GD, Rumley A, Wannamethee SG, Welsh P, Whincup PH
J. Thromb. Haemost. 2011 9:1452-9
Species: Human
Sample Type: Serum
High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality.
Johansson PI, Larsen CF, Ostrowski SR, Perner A, Sorensen AM, Wanscher M, Welling KL
J. Thromb. Haemost. 2011 10:207-16
Species: Human
Sample Type: Plasma
Pre-operative platelet-bound CD40 ligand is probably associated with peri-operative cardiac events in hip and knee arthroplasty.
Calabro LJ, Crawford RW, Crawford SA, Ray MJ, Sirisena T, Walters DL
Eur. J. Clin. Invest. 2010 40:497-503
Species: Human
Sample Type: Plasma
Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
Barr RG, Johnson WC, Kawut SM
Biomarkers 2010 15:731-8
Species: Human
Sample Type: Plasma
Platelet turnover, coagulation factors, and soluble markers of platelet and endothelial activation in essential thrombocythemia: relationship with thrombosis occurrence and JAK2 V617F allele burden.
Alvarez-Larran A, Arellano-Rodrigo E, Bellosillo B, Cervantes F, Colomer D, Reverter JC, Villamor N
Am. J. Hematol. 2009 84:102-8
Species: Human
Sample Type: Plasma
Interleukin-18 is a strong predictor of cardiovascular events in elderly men with the metabolic syndrome: synergistic effect of inflammation and hyperglycemia.
Arnesen H, Hjerkinn EM, Seljeflot I, Troseid M
Diabetes Care 2009 32:486-92
Species: Human
Sample Type: Plasma
A plant stanol yogurt drink alone or combined with a low-dose statin lowers serum triacylglycerol and non-HDL cholesterol in metabolic syndrome patients.
Brufau G, Dallinga-Thie GM, Dasselaar M, Mensink RP, Plat J
J. Nutr. 2009 139:1143-9
Species: Human
Sample Type: Plasma
Validity of multiplex-based assays for cytokine measurements in serum and plasma from "non-diseased" subjects: comparison with ELISA.
Achaintre D, Becker S, Dossus L, Kaaks R, Rinaldi S
J. Immunol. Methods 2009 350:125-32
Species: Human
Sample Type: Plasma
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