Human CD40 Quantikine ELISA Kit | hCD40 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111048
R&D Systems製のHuman CD40を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月17日 00時00分現在]
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CD40, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年04月17日 00時00分現在]
CD40, Human, ELISA Kit, Quantikine (96well)
文献数： 0
- 商品コード：DCCD40
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：5.56 pg/mL，測定範囲：19.50 - 1250 pg/mL，種交差性：Human，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Saliva (100 uL), Urine (20 uL), Human Milk (50 uL)，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC60，測定時間：4.5 hours，測定因子：CD40/TNFRSF5 別名：B cell surface antigen CD40 Genbank No： 958 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Quantikine CD40 ELISA Kit Immune Checkpoint Protein ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Saliva (100 µL), Urine (20 µL), Human Milk (50 µL)
- Sensitivity
- 5.56 pg/mL
- Assay Range
- 19.5 - 1,250 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human CD40
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human CD40 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CD40 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human CD40 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD40 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD40.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 97.5 | 301 | 561 | 104 | 307 | 574 |
| Standard Deviation | 4.65 | 14.7 | 24.2 | 9.53 | 19.6 | 29.9 |
| CV% | 4.8 | 4.9 | 4.3 | 9.2 | 6.4 | 5.2 |
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Recovery
The recovery of CD40 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 105 | 95-115 |
| Serum (n=4) | 92 | 85-101 |
| EDTA Plasma (n=4) | 92 | 87-104 |
| Heparin Plasma (n=4) | 93 | 82-102 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CD40 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CD40/TNFRSF5
- Aliases
- CD40; TNFRSF5
- Entrez Gene IDs
- 958 (Human); 21939 (Mouse); 171369 (Rat)
- Background
- CD40/TNFRSF5
- CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD40 is expressed on B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells and several tumor cell lines.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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