Human VE-Cadherin Quantikine ELISA Kit | hVE-Cadherin QKit
掲載日情報：2016/08/18 現在Webページ番号：111040
R&D Systems製のHuman VE-Cadherinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年07月17日 09時55分現在]
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Human VE-Cadherin Quantikine ELISA Kit |
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[在庫・価格 ：2025年07月17日 09時55分現在]
Human VE-Cadherin Quantikine ELISA Kit
文献数： 4
- 商品コード：DCADV0
- メーカー：RSD
- 包装：1kit
- 価格：¥98,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：1.56-100 ng/mL，感度：0.236 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC184，測定時間：4.5 hours，測定因子：VE-Cadherin 別名：7B4 Genbank No： 1003 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human VE-Cadherin Quantikine(R) ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.236 ng/mL
- Assay Range
- 1.56 - 100 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human VE-Cadherin
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human VE-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VE-Cadherin in cell culture supernates, serum, and plasma. It contains NS0-derived recombinant human VE-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VE-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VE-Cadherin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 13.4 | 34.6 | 65.4 | 12.8 | 31.8 | 64.5 |
| Standard Deviation | 0.68 | 1.63 | 2.05 | 0.92 | 1.64 | 2.86 |
| CV% | 5.1 | 4.7 | 3.1 | 7.2 | 5.1 | 4.4 |
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Recovery
The recovery of VE-Cadherin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 101 | 94-108 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of VE-Cadherin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- VE-Cadherin
- Aliases
- Cadherin-5; CD144; CDH5
- Entrez Gene IDs
- 1003 (Human); 12562 (Mouse)
- Background
- Cadherins
- The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2+-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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