Human Apolipoprotein B/ApoB Quantikine ELISA Kit | hApoB Qkit
掲載日情報：2016/08/18 現在Webページ番号：111017
R&D Systems製のHuman Apolipoprotein B/ApoBを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月06日 00時00分現在]
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Human Apolipoprotein B/ApoB Quantikine ELISA Kit |
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[在庫・価格 ：2024年05月06日 00時00分現在]
Human Apolipoprotein B/ApoB Quantikine ELISA Kit
文献数： 5
- 商品コード：DAPB00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
種交差性：Human，感度：9.97 ng/mL，測定範囲：39.10 - 2500 ng/mL，測定試料：Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC45，測定時間：4.5 hours，測定因子：Apolipoprotein B/ApoB 別名：Apo B-100 Genbank No： 338 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Human Apolipoprotein B ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 9.97 ng/mL
- Assay Range
- 39.1 - 2,500 ng/mL (Serum, Heparin Plasma, EDTA Plasma)
- Specificity
- Natural human ApoB
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Apolipoprotein B/ApoB Immunoassay is a 4.5 hour solid phase ELISA designed to measure ApoB levels in serum and plasma.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 284 | 654 | 1,228 | 261 | 717 | 1,375 |
| Standard Deviation | 15 | 33.6 | 48.1 | 27.4 | 61.2 | 90.5 |
| CV% | 5.3 | 5.1 | 3.9 | 10.5 | 8.5 | 6.6 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of ApoB were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Apolipoprotein B/ApoB
- Aliases
- APOB; FLDB
- Entrez Gene IDs
- 338 (Human); 238055 (Mouse); 54225 (Rat)
- Background
- Apolipoproteins
- The apolipoproteins are a structurally-unrelated group of proteins that have some association with the transport of lipids in blood. Apolipoproteins, plus phospholipids, cholesterol and triglycerides, form spherical particles with a lipid/hydrophobic center and a (apolipo)protein coat. The apolipoprotein coat promotes aqueous solubility and serves as a ligand for lipoprotein receptors. HDL may contain apolipoproteins A, C, D, E, J, L and M, while LDL contains apolipoproteins B and E.ApoAI and ApoA2 are major protein components of serum high-density lipoprotein (HDL) and are produced by the liver and small intestine. They are involved in reverse cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 aa in length. It binds LDL to the ApoB/E receptor. ApoC activates lipoprotein lipase and may self-associate to form amyloid-type fibrils.ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders, particularly Alzheimer's disease.Serum amyloid A proteins (SAAs) are a family of homologous apolipoproteins of high density lipoprotein (HDL). They can be divided into two groups. The first group consists of the acute phase SAA1 and SAA2 that associate with HDL during inflammation and remodel the HDL particle by displacing apolipoprotein A1. The second group consists of constitutively expressed SAA4 and SAA5 that exist as minor apolipoproteins on HDL but make up more than 90% of the total SAA during homeostasis.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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