Human Angiopoietin-2 Quantikine ELISA Kit | hAng-2 QKit

掲載日情報:2016/08/18 現在Webページ番号:111015

R&D Systems製のHuman Angiopoietin-2を測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2024年07月17日 20時55分現在]

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Angiopoietin-2, Human, ELISA Kit, Quantikine (96 well)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 99
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説明文
測定範囲:46.90 - 3000 pg/mL,感度:21.3 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL),種交差性:Human,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC43,測定時間:4.5 hours,測定因子:Angiopoietin-2
別名:AGPT2
Genbank No: 285
別包装品 別包装品あり
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2019年12月15日号 p.6

製品記事 Quantikine Angiopoietin-2 ELISA Kit
ELISA Kit製品に関するFAQ(R&D Systems)
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

[在庫・価格 :2024年07月17日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Angiopoietin-2, Human, ELISA Kit, Quantikine (96 well)

文献数: 99

説明文 測定範囲:46.90 - 3000 pg/mL,感度:21.3 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL),種交差性:Human,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC43,測定時間:4.5 hours,測定因子:Angiopoietin-2
別名:AGPT2
Genbank No: 285
別包装品 別包装品あり
法規制等
保存条件 4℃ 法規備考
掲載カタログ ニュース2019年12月15日号 p.6

製品記事 Quantikine Angiopoietin-2 ELISA Kit
ELISA Kit製品に関するFAQ(R&D Systems)
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (50 µL)

Sensitivity
21.3 pg/mL
Assay Range
46.9 - 3,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, EDTA Plasma)
Specificity
Natural and recombinant human Angiopoietin-2
Interference
No significant interference observed with available related molecules.

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Product Summary

The Quantikine Human Angiopoietin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Angiopoietin-2 in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human Angiopoietin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Angiopoietin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Angiopoietin-2.

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Precision

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 237 703 1,301 276 805 1,494
Standard Deviation 16.4 45.6 54.4 28.8 73 111
CV% 6.9 6.5 4.2 10.4 9.1 7.4

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Recovery

The recovery of Angiopoietin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=4) 100 90-107
Heparin Plasma (n=4) 94 87-104
EDTA Plasma (n=4) 95 85-104
Cell Culture Media (n=4) 103 98-113

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Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Additional Infomation

Molecule Information
Angiopoietin-2
Aliases
ANGPT2
Entrez Gene IDs
285 (Human); 11601 (Mouse)
Background
Angiopoietins
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
Amadori D, Arvanitakis C, Badulescu F, Barrios C, Beaugrand M, Befeler A, Bennouna J, Blanc J-, Blum H, Bolondi L, Borbath I, Boyer M, Boyer T, Britten C, Bronowicki J-, Bruix J, Buggisch P, Burak K, Bustamante Schneider J, Byrne T, Caspary W, Cisnero Garza LE, Cognetti F, Cosme de Oliveira A, Craxi A, De Greve J, Degos F, Diago M, Dollinger M, Dominguez S, Falk S, Farinati F, Figer A, Forner A, Gamargo Garate C, Gane E, Garcia-Tsao G, Gerken G, Germanidis G, Goke B, Gold P, Goldenberg A, Grange J-, Gregor M, Greten T, Gridelli C, Haussinger D, Heuman D, Hilgard P, Hillon P, Jassem J, Jeffry Evans TR, Katsos I, Kennedy P, Koch A, Kotzev I, Kubyshkin V, Lathia CD, Leon J, Llovet JM, Lozano A, Ma M, Marrero J, Martinez-Castillo J, Martoni A, Mazzaferro V, Meinhardt G, Metrakos P, Meyer T, Miron L, Montero Alvarez JL, Muellhaupt B, O'Donnell A, Olweny C, Pallota MG, Pascual S, Pena CE, Porta C, Raoul J-, Reeves H, Ricci S, Riordan S, Ross P, Roth A, Ru??z del Arbol L, Rydzewska G, Sangiovanni A, Sangro B, Santoro A, Scher??bl J, Scheulen M, Schilsky M, Schmid R, Schwartz J, Schwartz M, Seitz J-, Shan M, Sherman M, Sol?? R, Spengler U, Stemmer S, Strickland A, Szawlowski A, Tabernero J, Takov D, Tchernev K, Tebbutt N, Thomson B, Tomczak P, Trevisani F, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Wiest R, Zarba JJ, Zeuzem S
Clin. Cancer Res. 2012 18:2290-300
Species: Human
Sample Type: Plasma


Intermediate monocytes but not TIE2-expressing monocytes are a sensitive diagnostic indicator for colorectal cancer.
Bachleitner-Hofmann T, Bergmann M, Brostjan C, Buchberger E, Gruenberger T, Jahn N, Reiter C, Schauer D, Starlinger P, Stift A, Zajc P
PLoS ONE 2012 7:e44450
Species: Human
Sample Type: Plasma


Impaired function of the Tie-2 receptor contributes to vascular leakage and lethality in anthrax.
Proc Natl Acad Sci U S A 2012 109:10024-9

Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
Currie MJ, Dachs GU, Robinson BA, Volkova E, Wells JE, Willis JA
Br. J. Cancer 2011 104:51-9
Species: Human
Sample Type: Serum


Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
Cheng K, Li TS, Malliaras K, Marban E, Marban L, Matsushita N, Sun B, Zhang Y
Cardiovasc. Res. 2011 89:157-65
Species: Human
Sample Type: Cell Culture Supernates


Circulating angiopoietin-2 and soluble Tie-2 in type 2 diabetes mellitus: a cross-sectional study.
Ilhan A, Kautzky-Willer A, Lampichler K, Rasul S, Reiter MH, Wagner L
Cardiovasc Diabetol 2011 10:55
Species: Human
Sample Type: Plasma


Effect of prostaglandin F2 alpha on local luteotropic and angiogenic factors during induced functional luteolysis in the bovine corpus luteum.
Berisha B, Meyer HH, Schams D
Biol. Reprod. 2010 82:940-7
Species: Bovine
Sample Type: Tissue Homogenates


Changes in circulating levels and ratios of angiopoietins during pregnancy but not during the menstrual cycle and controlled ovarian stimulation.
Hurliman AK, Lee A, Molskness TA, Patton PE, Speroff L, Stouffer RL
Fertil. Steril. 2010 93:1493-9
Species: Human
Sample Type: Serum


Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis.
Adamyan L, Bourlev V, Iljasova N, Larsson A, Olovsson M
Fertil. Steril. 2010 94:52-7
Species: Human
Sample Type: Serum


Defective angiogenesis in hypoplastic human fetal lungs correlates with nitric oxide synthase deficiency that occurs despite enhanced angiopoietin-2 and VEGF.
Benachi A, Boucherat O, Bourbon JR, Delacourt C, Elie C, Franco-Montoya ML, Martinovic J, Masse V, Thebaud B
Am. J. Physiol. Lung Cell Mol. Physiol. 2010 298:L849-56
Species: Human
Sample Type: Tissue Homogenates


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