Human ADAM12 Quantikine ELISA Kit | hADAM12 QKit
掲載日情報：2016/08/18 現在Webページ番号：111008
R&D Systems製のHuman ADAM12を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 00時00分現在]
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ADAM12, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月26日 00時00分現在]
ADAM12, Human, ELISA Kit, Quantikine (96well)
文献数： 2
- 商品コード：DAD120
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：0.156-10 ng/mL，感度：0.076 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC34，測定時間：4.5 hours，測定因子：ADAM12 別名：a disintegrin and metalloproteinase domain 12 (meltrin alpha) Genbank No： 8038 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human ADAM12 Quantikine(R) ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (25 µL), Heparin Plasma (25 µL), Urine (50 µL)
- Sensitivity
- 0.076 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, Urine)
- Specificity
- Natural and recombinant human ADAM12. This kit recognizes pro-ADAM12, mature ADAM12, and ADAM12/TIMP-3 complex.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human ADAM12 immunoassay is a 4.5 hour solid phase ELISA designed to measure human ADAM12 in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human ADAM12 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural ADAM12 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ADAM12.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.39 | 3.57 | 6.84 | 1.43 | 3.62 | 6.74 |
| Standard Deviation | 0.057 | 0.131 | 0.235 | 0.079 | 0.145 | 0.299 |
| CV% | 4.1 | 3.7 | 3.4 | 5.5 | 4 | 4.4 |
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Recovery
The recovery of human ADAM12 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 98 | 89-106 |
| Heparin Plasma (n=4) | 97 | 92-106 |
| Urine (n=4) | 99 | 91-106 |
| Cell Culture Media (n=8) | 98 | 88-105 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ADAM12 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- ADAM12
- Aliases
- Meltrin alpha
- Entrez Gene IDs
- 8038 (Human)
- Background
- ADAMs (A Disintegrin and Metalloprotease)
- Members of the ADAM or MDC (Metalloprotease, Disintegrin, Cysteine-rich) family contain pro, metalloprotease-like, disintegrin-like, cysteine-rich, transmembrane and cytoplasmic domains. They play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Evaluation of ADAM-12 as a Diagnostic Biomarker of Ectopic Pregnancy in Women with a Pregnancy of Unknown Location.
Brown JK, Horne AW, Kaitu'u-Lino T, Tong S
PLoS ONE 2012 7:e41442
Species: Human
Sample Type: Serum
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