Human CXCL8/IL-8 Quantikine ELISA Kit | hIL-8 QKit
掲載日情報：2016/08/18 現在Webページ番号：111004
R&D Systems製のHuman CXCL8/IL-8を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月30日 00時00分現在]
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IL-8, Human, ELISA Kit, Quantikine, 3rd Generation (96well) <CXCL8, ELISA Kit> |
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[在庫・価格 ：2024年04月30日 00時00分現在]
IL-8, Human, ELISA Kit, Quantikine, 3rd Generation (96well) <CXCL8, ELISA Kit>
文献数： 513
- 商品コード：D8000C
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：31.20 - 2000 pg/mL，感度：7.5 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC23，測定時間：3.5 hours，測定因子：CXCL8/IL-8 別名：3-10C Genbank No： 3576 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 |
IL-8 ELISA Kit／抗体／換え体タンパク質 新型コロナウイルス感染に起因するサイトカインストームのモニタリング用製品 |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL)
- Sensitivity
- 7.5 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-8
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human IL-8 Immunoassay is a 3.5 hour solid phase ELISA designed to measure human IL-8 in cell culture supernates, serum, and plasma. It is based on antibodies raised against the 72 aa variant of human IL-8 derived from E. coli. It is calibrated with the same recombinant factor. This immunoassay accurately quantitates recombinant human IL-8. Measurement of natural human IL-8 or the 77 aa variant of human IL-8 gave results parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
hree samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 168 | 526 | 1,093 | 196 | 581 | 1,160 |
| Standard Deviation | 9.4 | 28.6 | 70.7 | 14.5 | 56.6 | 70.2 |
| CV% | 5.6 | 5.4 | 6.5 | 7.4 | 9.7 | 6.1 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 115 | 386 | 802 | 132 | 410 | 817 |
| Standard Deviation | 5.3 | 17 | 37.9 | 10.7 | 28 | 42.4 |
| CV% | 4.6 | 4.4 | 4.7 | 8.1 | 6.8 | 5.2 |
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Recovery
The recovery of IL-8 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=5) | 98 | 85-114 |
| Serum (n=5) | 98 | 88-106 |
| EDTA Plasma (n=5) | 103 | 97-111 |
| Heparin Plasma (n=5) | 102 | 92-107 |
| Citrate Plasma (n=5) | 105 | 95-114 |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-8 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CXCL8/IL-8
- Aliases
- GCP1; IL8; LAI; MDNCF; NAP1; NCF; TCF; TSG1
- Entrez Gene IDs
- 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
- Background
- CXCL8/IL-8
- CXCL8 was originally discovered and purified as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocytederived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor-4, GRO, and IP10.CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other proinflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
Chang , Luping, Hsu , Chia-Lin, Huang , Fong-Lee, Lai , Tzu-Hsua, Shiao , Young-Ji, Tsay , Huey-Jen, Tzeng , Tsai-Ten
J Biomed Sci 2013 20:90
Species: Human
Sample Type: Cell Culture Supernates
A randomized trial of recombinant human granulocyte-macrophage colony stimulating factor for patients with acute lung injury.
Brown MB, Burnham EL, Dechert RE, Hyzy RC, Martin GS, Moss M, Paine R, Rosenberg AL, Standiford TJ, Thannickal VJ
Crit. Care Med. 2012 40:90-7
Species: Human
Sample Type: BALF
Alveolar fibrocyte percentage is an independent predictor of poor outcome in patients with acute lung injury.
Bouadma L, Crestani B, Dehoux M, Elbim C, Garnier M, Gelly J, Lasocki S, Lecon V, Mentre F, Nardelli L, Philip I, Piednoir P, Quesnel C, Soler P
Crit. Care Med. 2012 40:21-8
Species: Human
Sample Type: BALF
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Caillat-Zucman S, Choukem SP, Dupuy S, Lambert M, Lebbe C, Pages C, Tognarelli S, Zucman D
PLoS Pathog. 2012 8:e1002486
Species: Human
Sample Type: Cell Culture Supernates
Late SV40 factor (LSF) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP-9).
Chen D, Gredler R, Santhekadur PK
J. Biol. Chem. 2012 287:3425-32
Species: Human
Sample Type: Cell Culture Supernates
Epithelial proinflammatory response and curcumin-mediated protection from staphylococcal toxic shock syndrome toxin-1.
Anderson MJ, Breshears LM, Grill AE, Lin YC, Panyam J, Peterson ML, Schaefers MM, Schlievert PM, Southern PJ
PLoS ONE 2012 7:e32813
Species: Porcine
Sample Type: Cell Culture Supernates
Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function.
Castleton AZ, Dey A, Elham M, Fielding AK, Ghorani E, Patel B, Rai L, Zhang Y
J. Immunol. 2012 188:1002-10
Biomarkers for antitumor activity of bevacizumab in gastric cancer models.
Fujimoto-Ouchi K, Kurasawa M, Mori K, Yamashita-Kashima Y, Yanagisawa M, Yasuno H, Yorozu K
BMC Cancer 2012 12:37
Species: Human
Sample Type: Tissue Homogenates
Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
Andersson M, Godaly G, Hallgren O, Lutay N, Svensson M, Westergren-Thorsson G
Innate Immun 2012 18:531-40
Species: Human
Sample Type: Cell Culture Supernates
A key role for the endothelium in NOD1 mediated vascular inflammation: comparison to TLR4 responses.
Bertin J, Gatheral T, Gough P, Lim E, Mitchell J, Moreno L, Nicholson A, Reed D, Rickard D, Sehon C, Votta B
PLoS ONE 2012 7:e42386
Species: Human
Sample Type: Cell Culture Supernates
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