Human IL-19 Quantikine ELISA Kit | hIL-19 QKit
掲載日情報：2016/08/18 現在Webページ番号：110994
R&D Systems製のHuman IL-19を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年07月17日 10時15分現在]
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IL-19, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年07月17日 10時15分現在]
IL-19, Human, ELISA Kit, Quantikine (96well)
文献数： 5
- 商品コード：D1900
- メーカー：RSD
- 包装：1kit
- 価格：¥98,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：31.2-2,000 pg/mL，感度：12.2 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Human Milk，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC24，測定時間：4.5 hours，測定因子：IL-19 別名：IL-10C Genbank No： 29949 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human IL-19 Quantikine® ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (50 µL), Human Milk (50 µL)
- Sensitivity
- 12.2 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human IL-19
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human IL-19 immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-19 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human IL-19 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-19 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-19.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 292 | 805 | 1,586 | 269 | 739 | 1,478 |
| Standard Deviation | 10.3 | 19 | 41.8 | 22 | 56.8 | 103 |
| CV% | 3.5 | 2.4 | 2.6 | 8.2 | 7.7 | 7 |
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Recovery
The recovery of human IL-19 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 93 | 87-97 |
| Heparin Plasma (n=4) | 93 | 87-99 |
| EDTA Plasma (n=4) | 94 | 85-102 |
| Cell Culture Media (n=4) | 98 | 93-104 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-19 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-19
- Aliases
- undefined
- Entrez Gene IDs
- 29949 (Human); 329244 (Mouse)
- Background
- IL-19
- Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. IL-19 contains seven helices and is secreted as a 35 kDa glycosylated monomer. IL-19 expression has been observed in keratinocytes and monocytes. IL-19 binds a receptor complex consisting of IL-20R alpha and the IL-20 R beta. This receptor complex is also shared by IL-20 and IL-24.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Crimean Congo hemorrhagic fever virus infects human monocyte-derived dendritic cells.
Connolly-Andersen AM, Douagi I, Kraus AA, Mirazimi A
Virology 2009 390:157-62
Species: Human
Sample Type: Cell Culture Supernates
The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
Asadullah K, Docke WD, Kunz S, Philipp S, Sabat R, Schulze-Tanzil G, Sterry W, Volk HD, Warszawska K, Witte E, Witte K, Wolk K
Eur. J. Immunol. 2009 39:3570-81
Species: Human
Sample Type: Serum
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