Human IL-17 Quantikine ELISA Kit | hIL-17 QKit
掲載日情報：2016/08/18 現在Webページ番号：110993
R&D Systems製のHuman IL-17を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月02日 00時00分現在]
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IL-17, Human, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2024年05月02日 00時00分現在]
IL-17, Human, ELISA Kit, Quantikine (96 well)
文献数： 85
- 商品コード：D1700
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：31.20 - 2000 pg/mL，感度：15 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC21，測定時間：4.5 hours，測定因子：IL-17/IL-17A 別名：CTLA-8 Genbank No： 3605 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 |
CellXVivo Human Th2 Cell Differentiation Kit R&D Systems Interleukin-17 (IL-17)測定キット |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
- Sensitivity
- 15 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-17
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human IL-17 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-17 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-17 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-17 accurately. Results obtained using natural human IL-17 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-17.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 131 | 736 | 1,480 | 125 | 408 | 794 |
| Standard Deviation | 5.8 | 34.6 | 61.2 | 9.8 | 34.3 | 55.6 |
| CV% | 4.4 | 4.7 | 4.1 | 7.8 | 8.4 | 7 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 107 | 604 | 1,217 | 76.6 | 272 | 549 |
| Standard Deviation | 7.8 | 25 | 63.3 | 6.5 | 23.4 | 43.9 |
| CV% | 7.3 | 4.1 | 5.2 | 8.5 | 8.6 | 8 |
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Recovery
The recovery of human IL-17 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 98 | 90-105 |
| Heparin Plasma (n=5) | 101 | 93-109 |
| Citrate Plasma (n=5) | 105 | 92-116 |
| EDTA Plasma (n=5) | 101 | 90-110 |
| Cell Culture Media (n=5) | 97 | 88-112 |
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Linearity
To assess the linearity of the assay, five samples spiked with high concentrations of IL-17 were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-17/IL-17A
- Aliases
- CTLA-8; IL17A
- Entrez Gene IDs
- 3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
- Background
- IL-17 Ligands
- The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Elevated serum levels of interleukin-17A in children with autism.
Al-Ayadhi LY, Mostafa GA
J Neuroinflammation 2012 9:158
Species: Human
Sample Type: Serum
Subnormal cytokine profile in the tear fluid of keratoconus patients.
Chakravarti S, Cope L, Feng X, Hamad A, Jun AS, Lee S, Meng H, Speck C
PLoS ONE 2011 6:e16437
Species: Human
Sample Type: Tears
Preclinical Evaluation of Local JAK1 and JAK2 Inhibition in Cutaneous Inflammation.
Burn T, Collier P, Collins R, Contel N, Fridman JS, Friedman SM, Haley P, Hertel D, Hollis G, Metcalf B, Neilan CL, Newton RC, Rodgers JD, Scherle PA, Shepard S, Shi J, Thomas B, Vaddi K, Yeleswaram S
J. Invest. Dermatol. 2011 131:1838-44
Species: Human
Sample Type: Cell Culture Supernates
Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration.
Agron E, Chakrabarty S, Chan CC, Chew E, Ferris F, Klein ML, Li Z, Liu B, Meyerle C, Nussenblatt RB, Sen HN, Tuo J, Wei L
J Transl Med 2011 9:111
Species: Human
Sample Type: Serum
Cytokine Response Patterns in Severe Pandemic 2009 H1N1 and Seasonal Influenza among Hospitalized Adults.
Chan MC, Chan PK, Choi KW, Hui DS, Lee N, Lee SK, Lui GC, Lun SW, Ngai KL, Wong BC, Wong CK, Wong RY
PLoS ONE 2011 6:e26050
Species: Human
Sample Type: Plasma
Identification of human NK17/NK1 cells.
Al-Jaderi Z, Harbo HF, Hoglund RA, Holmoy T, Maghazachi AA, Norgauer J, Pandya AD
PLoS ONE 2011 6:e26780
Species: Human
Sample Type: Cell Culture Supernates
IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Asano Y, Fujita H, Kadono T, Kamata M, Miyagaki T, Ohmatsu H, Sato S, Suga H, Sugaya M, Tada Y
Clin. Cancer Res. 2011 17:7529-38
Species: Human
Sample Type: Serum
Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
Anderson IK, Bafadhel M, Brightling CE, Desai D, Doe C, May R, McCormick M, Mistry V, Rugman P, Siddiqui S, Sleeman MA, Woods J
Chest 2011 138:1140-7
Species: Human
Sample Type: Sputum
Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients.
Morishige R, Nakajima H, Nakajima K, Sano S, Tarutani M
Arch. Dermatol. Res. 2011 303:451-5
Species: Human
Sample Type: Serum
An antiinflammatory dietary mix modulates inflammation and oxidative and metabolic stress in overweight men: a nutrigenomics approach.
Bakker GC, Cnubben NH, Hendriks HF, Kooistra T, Pellis L, Rubingh CM, van Erk MJ, van Ommen B, Wopereis S
Am. J. Clin. Nutr. 2010 91:1044-59
Species: Human
Sample Type: Plasma
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