Canine IFN-gamma Quantikine ELISA Kit | caIFN-g Qkit
掲載日情報：2016/08/18 現在Webページ番号：110984
R&D Systems製のCanine IFN-gammaを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 17時55分現在]
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IFN-γ, Canine, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月26日 17時55分現在]
IFN-γ, Canine, ELISA Kit, Quantikine (96well)
文献数： 11
- 商品コード：CAIF00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：62.5-4,000 pg/mL，感度：60 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Canine，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4 hours，測定因子：IFN-gamma 別名：IFG Genbank No： 3458 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Canine/Feline IFN-γ Quantikine/DuoSet ELISA Kit Helper T Cells Marker Antibodies ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 60 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant canine IFN-gamma
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Canine IFN-gamma Immunoassay is a 4 hour solid-phase ELISA designed to measure canine IFN-gamma in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring canine IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine IFN-gamma.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 111 | 459 | 1,234 | 119 | 457 | 1,315 |
| Standard Deviation | 10.1 | 35.1 | 92.4 | 10.1 | 21.5 | 64.8 |
| CV% | 9.1 | 7.6 | 7.5 | 8.5 | 4.7 | 4.9 |
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Recovery
The recovery of canine IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 96 | 83-107 |
| Heparin Plasma (n=4) | 98 | 93-106 |
| Cell Culture Supernates (n=4) | 103 | 92-111 |
| EDTA Plasma (n=4) | 96 | 86-102 |
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of canine IFN-gamma in each matrix were diluted with Calibrator Diluent and assayed.
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Additional Infomation
- Molecule Information
- IFN-gamma
- Aliases
- IFNG
- Entrez Gene IDs
- 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
- Background
- IFN-gamma
- Interferon-γ (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells.IFN-γ was originally characterized based on its antiviral activities. The protein also exerts anti-proliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, up-regulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments Helper T cell expansion and may be required for Helper T cell differentiation.IFN-γ exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-γ receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN-γ receptor is structurally related to the IL-10 receptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Identification of myeloid derived suppressor cells in dogs with naturally occurring cancer.
Goulart MR, Ohlfest JR, Pluhar GE
PLoS ONE 2012 7:e33274
Species: Canine
Sample Type: Cell Culture Supernates
Human Flt3L generates dendritic cells from canine peripheral blood precursors: implications for a dog glioma clinical trial.
Candolfi M, Liu C, Xiong W
PLoS ONE 2010 5:e11074
Species: Canine
Sample Type: Cell Culture Supernates
Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs.
Bannai H, Fukumoto S, Igarashi I, Okamura M, Suzuki H, Suzuki T, Tamaki Y, Xuan X, Yokoyama N
Vaccine 2007 25:1334-41
Species: Canine
Sample Type: Serum
Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Hijikata T, Howell JM, Miyagoe-Suzuki Y, Nakamura A, Ohshima S, Takeda S, Urasawa N, Yoshimura M, Yuasa K
Gene Ther. 2007 14:1249-60
Species: Canine
Sample Type: Cell Culture Supernates
A functional comparison of canine and murine bone marrow derived cultured mast cells.
, Lin TY, London CA
Vet. Immunol. Immunopathol. 2006 114:320-34
Species: Canine
Sample Type: Cell Culture Supernates
Effect of ovarian hormones on periodical changes in immune resistance associated with estrous cycle in the beagle bitch.
Hatoya S, Ikehara S, Inaba M, Inaba T, Ishiguro K, Kumagai D, Nishikawa M, Sawada T, Sugiura K, Tajima T, Tamada H, Torii R, Wijewardana V
Immunobiology 2004 209:619-27
Species: Canine
Sample Type: Cell Culture Supernates
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