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Serum-free Media for Human Lymphocytes ALyS series ALyS™505N / ALyS™705

Date：November 29 2020Web Page No：95004

Cell Science & Technology Institute, Inc.

ALyS series provided by Cell Science & Technology Institute is serum-free medium developed for in vitro culture of human peripheral blood lymphocytes. T lymphocyte culture is important in various immunological and clinical research. Research is underway to improve the cytocidal activity of T lymphocytes isolated from peripheral blood and natural killer (NK) cells. Various lymphocytes such as activated lymphokine-activated killer cells (LAK), cytotoxic T lymphocytes (CTL), and NK cells are used for immunocytotherapy.
ALyS™505N Medium : Serum-free and developed for in vitro culture of human peripheral blood lymphocytes.
ALyS™705 Medium : Chemically defined, xeno-free and developed for in vitro culture of human peripheral blood lymphocytes.

AlyS505N-705

Product Line-up

Basic composition: IMDM medium, HEPES, L-glutamine, lipids, sodium bicarbonate, sodium selenite, 2-aminomethanol

Name Code Features pH Insulin,
recombinant		 Transferrin,
recombinant		 Albumin IL-2
(IU/ml)
ALyS505N-0 1020P10 • Serum-free
• Xeno-free
• All proteins contained are human type.		 7.3-7.5 ✓ ✓ ✓ -
ALyS505N-175 10217P10 ✓ ✓ ✓ 175
ALyS505N-7 1027P10 ✓ ✓ ✓ 700
ALyS505N-10 10210P10 ✓ ✓ ✓ 1,000
ALyS705 120P10 • Chemically defined medium
• Xeno-free		 7.3-7.7 ✓ ✓ - -

* ✓ means it contains.


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ALyS™505N Medium

Features

  • Suitable for culturing human peripheral T-lymphocytes
  • Xeno-free: all proteins contained in the medium are recombinants or human proteins with heat-treatment.
  • High growth rate of human peripheral T-lymphocytes: adding small amount of plasma (0.1-2%) may help to increase the growth rate
*Note: In case of primary culture of peripheral blood T-lymphocytes, it is recommended to add 1-5% plasma.

Reference data

referencedata
Proliferation of anti-CD3 activated lymphocytes

Product information

[Date : April 25 2025 00:06]

Detail Product Name Product Code Supplier Size Price
ALyS505N-0 Medium,w/o IL-2 (#87-661)
DatasheetThis may not be the latest data sheet. 		1020P10 KSKCell Science & Technology Institute, Inc. 1000 ml $91

Description New code: 87-661, Modified ALyS-Basal medium for serum-free culture of human T-lymphocytes and Lymphoblast cells. Particularly designed for the research of cancer Immuno-therapy.
Storage 4°C,Dark Strage CAS
Link

Lymactin-T

ALyS505N-175 Medium,w/ IL-2(175IU/ml) (#87-654)
DatasheetThis may not be the latest data sheet. 		10217P10 KSKCell Science & Technology Institute, Inc. 1000 ml $110

Description New code: 87-654, ALyS505N-0 medium containing IL-2(175IU/ml). Particularly designed for the research of Cancer Immuno-therapy.
Storage 4°C,Dark Strage,Don't freeze CAS
Link

Lymactin-T

ALyS505N-7 Medium, w/ IL-2 (700IU/ml) (#87-666)
DatasheetThis may not be the latest data sheet. 		1027P10 KSKCell Science & Technology Institute, Inc. 1000 ml $194

Description New code: 87-666
Storage 4°C,Dark Strage,Don't freeze CAS
Link

Lymactin-T

ALyS505N-10 Medium,w/ IL-2(1,000IU/ml) (#87-676)
DatasheetThis may not be the latest data sheet. 		10210P10 KSKCell Science & Technology Institute, Inc. 1000 ml $289

Description New code: 87-676, ALyS505N-0 medium containing IL-2(1,000IU/ml). Particularly designed for the research of Cancer Immuno-therapy by LAK.
Storage 4°C CAS
Link

Lymactin-T

ALyS505N-0 B10 IL-2(-) (filled in culture bag) *SPQ(Standard Packing Quantity):10*
 87-669 KSKCell Science & Technology Institute, Inc. 1000 ml $127

Description SPQ(Standard Packing Quantity):10
Storage 4°C CAS
Link

[Date : April 25 2025 00:06]

ALyS505N-0 Medium,w/o IL-2 (#87-661)

DatasheetThis may not be the latest data sheet.


  • Product Code: 1020P10
  • Supplier: KSK
  • Size: 1000ml
  • Price: $91

Description New code: 87-661, Modified ALyS-Basal medium for serum-free culture of human T-lymphocytes and Lymphoblast cells. Particularly designed for the research of cancer Immuno-therapy.
Storage 4°C,Dark Strage CAS
Link

Lymactin-T

ALyS505N-175 Medium,w/ IL-2(175IU/ml) (#87-654)

DatasheetThis may not be the latest data sheet.


  • Product Code: 10217P10
  • Supplier: KSK
  • Size: 1000ml
  • Price: $110

Description New code: 87-654, ALyS505N-0 medium containing IL-2(175IU/ml). Particularly designed for the research of Cancer Immuno-therapy.
Storage 4°C,Dark Strage,Don't freeze CAS
Link

Lymactin-T

ALyS505N-7 Medium, w/ IL-2 (700IU/ml) (#87-666)

DatasheetThis may not be the latest data sheet.


  • Product Code: 1027P10
  • Supplier: KSK
  • Size: 1000ml
  • Price: $194

Description New code: 87-666
Storage 4°C,Dark Strage,Don't freeze CAS
Link

Lymactin-T

ALyS505N-10 Medium,w/ IL-2(1,000IU/ml) (#87-676)

DatasheetThis may not be the latest data sheet.


  • Product Code: 10210P10
  • Supplier: KSK
  • Size: 1000ml
  • Price: $289

Description New code: 87-676, ALyS505N-0 medium containing IL-2(1,000IU/ml). Particularly designed for the research of Cancer Immuno-therapy by LAK.
Storage 4°C CAS
Link

Lymactin-T

ALyS505N-0 B10 IL-2(-) (filled in culture bag) *SPQ(Standard Packing Quantity):10*


  • Product Code: 87-669
  • Supplier: KSK
  • Size: 1000ml
  • Price: $127

Description SPQ(Standard Packing Quantity):10
Storage 4°C CAS
Link




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ALyS™705 Medium

Features

  • Chemically defined medium suitable for culturing human lymphocytes
  • Xeno-free: protein in the medium is only human-insulin recombinant
  • Higher growth rate than ALyS505N medium
  • Share stress between cells are decreased by micromolecular polymer used as a substitute of Albumin
  • Mouse acute toxicity was tested and did not show adverse effects on tissue

Reference data

referencedata
Proliferation of lymphocytes by ALys medium

Product information

[Date : April 25 2025 00:06]

Detail Product Name Product Code Supplier Size Price
ALyS705 Medium, w/o IL-2 (#87-016)
 120P10 KSKCell Science & Technology Institute, Inc. 1000 ml $227

Description New code: 87-016
Storage 4°C,Don't freeze CAS
Link

[Date : April 25 2025 00:06]

ALyS705 Medium, w/o IL-2 (#87-016)


  • Product Code: 120P10
  • Supplier: KSK
  • Size: 1000ml
  • Price: $227

Description New code: 87-016
Storage 4°C,Don't freeze CAS
Link



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