Animal-free Media for Human Lymphocytes ALyS series ALyS™505N / ALyS™705
Date:November 29 2020Web Page No:95004
Cell Science & Technology Institute, Inc.
ALyS series provided by Cell Science & Technology Institute is serum-free medium developed for in vitro culture of human peripheral blood lymphocytes. T lymphocyte culture is important in various immunological and clinical research. Research is underway to improve the cytocidal activity of T lymphocytes isolated from peripheral blood and natural killer (NK) cells. Various lymphocytes such as activated lymphokine-activated killer cells (LAK), cytotoxic T lymphocytes (CTL), and NK cells are used for immunocytotherapy.
ALyS™505N Medium : Serum-free and developed for in vitro culture of human peripheral blood lymphocytes.
ALyS™705 Medium : Chemically defined, xeno-free and developed for in vitro culture of human peripheral blood lymphocytes.

Product Line-up
Basic composition: IMDM medium, HEPES, L-glutamine, lipids, sodium bicarbonate, sodium selenite, 2-aminomethanol
Name | Code | Features | pH | Insulin, recombinant |
Transferrin, recombinant |
Albumin | IL-2 (IU/ml) |
ALyS505N-0 | 87-661 (Old: 1020P10) | • Serum-free • Xeno-free • All proteins contained are human type. |
7.3-7.5 | ✓ | ✓ | ✓ | 0 |
ALyS505N-175 | 87-654 (Old: 10217P10) | ✓ | ✓ | ✓ | 175 | ||
ALyS505N-7 | 87-666 (Old: 1027P10) | ✓ | ✓ | ✓ | 700 | ||
ALyS505N-10 | 87-676 (Old: 10210P10) | ✓ | ✓ | ✓ | 1,000 | ||
ALyS705 | 87-016 (Old: 120P10) | • Chemically defined medium • Xeno-free |
7.3-7.7 | ✓ | ✓ | - | 0 |
* ✓ means it contains.
ALyS™505N Medium
Features
- Suitable for culturing human peripheral T-lymphocytes
- Xeno-free: all proteins contained in the medium are recombinants or human proteins with heat-treatment.
- High growth rate of human peripheral T-lymphocytes: adding small amount of plasma (0.1-2%) may help to increase the growth rate
Reference data

Product information
[Date : June 05 2025 00:07]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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ALyS505N-0 Medium, w/o IL-2 (#1020P10) DatasheetThis may not be the latest data sheet. |
87-661 | KSKCell Science & Technology Institute, Inc. | 1000 ml | $91 | |||||||||||||||||||||||||||||||
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ALyS505N-175 Medium, w/ IL-2 (175IU/ml) (#10217P10) DatasheetThis may not be the latest data sheet. |
87-654 | KSKCell Science & Technology Institute, Inc. | 1000 ml | $110 | |||||||||||||||||||||||||||||||
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ALyS505N-7 Medium, w/ IL-2 (700IU/ml) (#1027P10) DatasheetThis may not be the latest data sheet. |
87-666 | KSKCell Science & Technology Institute, Inc. | 1000 ml | $194 | |||||||||||||||||||||||||||||||
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ALyS505N-10 Medium, w/ IL-2 (1,000IU/ml) (#10210P10) DatasheetThis may not be the latest data sheet. |
87-676 | KSKCell Science & Technology Institute, Inc. | 1000 ml | $289 | |||||||||||||||||||||||||||||||
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ALyS505N-0 B10 IL-2(-) (filled in culture bag) *SPQ(Standard Packing Quantity):10* DatasheetThis may not be the latest data sheet. |
87-669 | KSKCell Science & Technology Institute, Inc. | 1000 ml | $127 | |||||||||||||||||||||||||||||||
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[Date : June 05 2025 00:07]
ALyS505N-0 Medium, w/o IL-2 (#1020P10)
DatasheetThis may not be the latest data sheet.
- Product Code: 87-661
- Supplier: KSK
- Size: 1000ml
- Price: $91
Description |
Old code: 1020P10, Modified ALyS-Basal medium for serum-free culture of human T-lymphocytes and Lymphoblast cells. Particularly designed for the research of cancer Immuno-therapy. |
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Storage | 4°C,Dark Strage,Don't freeze | CAS | |
Link |
ALyS505N-175 Medium, w/ IL-2 (175IU/ml) (#10217P10)
DatasheetThis may not be the latest data sheet.
- Product Code: 87-654
- Supplier: KSK
- Size: 1000ml
- Price: $110
Description |
Old code: 2500P10, ALyS505N-0 medium containing IL-2(175IU/ml). Particularly designed for the research of Cancer Immuno-therapy. |
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Storage | 4°C,Dark Strage,Don't freeze | CAS | |
Link |
ALyS505N-7 Medium, w/ IL-2 (700IU/ml) (#1027P10)
DatasheetThis may not be the latest data sheet.
- Product Code: 87-666
- Supplier: KSK
- Size: 1000ml
- Price: $194
Description |
Old code: 1027P10 |
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Storage | 4°C,Dark Strage,Don't freeze | CAS | |
Link |
ALyS505N-10 Medium, w/ IL-2 (1,000IU/ml) (#10210P10)
DatasheetThis may not be the latest data sheet.
- Product Code: 87-676
- Supplier: KSK
- Size: 1000ml
- Price: $289
Description |
Old code: 10210P10, ALyS505N-0 medium containing IL-2(1,000IU/ml). Particularly designed for the research of Cancer Immuno-therapy by LAK. |
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Storage | 4°C,Dark Strage,Don't freeze | CAS | |
Link |
ALyS505N-0 B10 IL-2(-) (filled in culture bag) *SPQ(Standard Packing Quantity):10*
DatasheetThis may not be the latest data sheet.
- Product Code: 87-669
- Supplier: KSK
- Size: 1000ml
- Price: $127
Description |
SPQ(Standard Packing Quantity):10 |
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Storage | 4°C | CAS | |
Link |
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ALyS™705 Medium
Features
- Chemically defined medium suitable for culturing human lymphocytes
- Xeno-free: protein in the medium is only human-insulin recombinant
- Higher growth rate than ALyS505N medium
- Share stress between cells are decreased by micromolecular polymer used as a substitute of Albumin
- Mouse acute toxicity was tested and did not show adverse effects on tissue
Reference data

Product information
[Date : June 05 2025 00:07]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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ALyS705 Medium, w/o IL-2 (#120P10) |
87-016 | KSKCell Science & Technology Institute, Inc. | 1000 ml | $227 | |||||||||||||||||||||||||||||||
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[Date : June 05 2025 00:07]
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