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Endoplasmic Reticulum (ER) staining dye for long-term live imaging ERseeing | Funakoshi

Date:July 27 2020Web Page No:81514

Funakoshi Co.,Ltd.

ERseeing is irreversible ER (Endoplasmic Reticulum) staining dye and suitable for long-term live imaging.
Compared to conventional ER staining dye such as Glibenclamide-based dye, ERseeing exhibits very low pharmacological effect and high retentivity which enables to visualize ER even after washout or medium change.

ERseeing Labeling dye of ER

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Background

Endoplasmic reticulum (ER) is the largest organelle in a cell and has unique and dynamic tubular or sheet structures.
ER plays essential roles in protein synthesis and carbohydrate metabolism, and is a traffic origin of secreted pathway proteins including the Golgi apparatus, exocytosis, plasma membrane and extracellular proteins.

The most conventional ER dye is based on Glibenclamide fluorophore conjugate.
Glibenclamide is known as a potent and specific inhibitor of the sulphonylurea receptors of ATP sensitive K+ channels.

However, this type of dye has problems as below.
-Negative pharmacological effect on K+ channel in ER.
-Washed out by wash step and medium change.

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ERseeing

ERseeing solves the above problems; it exhibits little effect on the ER functions pharmacologically, and enables to visualize ER even after washout or medium change.

ERseeing has two units, a rhodol type green fluorescent dye and a thioester type protein labeling group.
Because this a rhodol type dye has a high affinity to ER membranes, right after adding the reagent to cells, it selectively and quickly accumulates into ER.
Once it accumulated in ER, the labeling group of ERseeing reacts with amino acids in ER-proteins randomly, and forms a covalent bond.

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Comparison between ERseeing and conventional ER dye

Comparison between ERseeing and conventional ER dye

Comparison of ER specificity

ERseeing Comparison of ER specificity

HeLa cells were stained with ERseeing (100 nM) and Glibencramide type ER staining.
ERseeing was highly overlapped with conventional Glibencramide type ER staining dye.

Comparison of retentivety

ERseeing Comparison of retentivety

HeLa cells were treated with ERseeing or Glibenclamide-based dye for 15 min and observed without wash (Left).
Both reagents show ER staining. After that, cells were washed by PBS, added fresh media containing 10% FBS and observed again.
While the Glibenclamide-based dye showed a very weak signal from the cells, ERseeing maintains a good signal from ER.
ERseeing is suitable for long term imaging after medium change.

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Absorption and fluorescent spectrum of ERseeing

Absorption and fluorescent spectrum of ERseeing

Excitation (blue) and fluorescent (red) spectrum.
Exmax /Emmax = 509/524 nm.
Commercial FITC filter sets are compatible.

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Product information

[Date : June 20 2024 00:07]

Detail Product Name Product Code Supplier Size Price
ERseeing, Endoplasmic Reticulum Green
DatasheetThis may not be the latest data sheet.
FDV-0038 FNAFunakoshi Co.,Ltd. 10 nmol $380

Description ERseeing is irreversible ER labeling dye and suitable for long-term live imaging.
Storage -20°C CAS
Link

"ERseeing" (Endoplasmic Reticulum Green Dye) in a minute!
ER-Protein Capture Kit | Funakoshi
Lipid Peroxidation Detection Tools for Cancer Research

[Date : June 20 2024 00:07]

ERseeing, Endoplasmic Reticulum Green


  • Product Code: FDV-0038
  • Supplier: FNA
  • Size: 10nmol
  • Price: $380

Description ERseeing is irreversible ER labeling dye and suitable for long-term live imaging.
Storage -20°C CAS
Link

"ERseeing" (Endoplasmic Reticulum Green Dye) in a minute!
ER-Protein Capture Kit | Funakoshi
Lipid Peroxidation Detection Tools for Cancer Research



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Related Product

Endoplasmic Reticulum (ER) proteins purification / isolation kit : ER-Protein Capture Kit

[Date : June 20 2024 00:07]

Detail Product Name Product Code Supplier Size Price
ER-Protein Capture Kit
DatasheetThis may not be the latest data sheet.
FDV-0039 FNAFunakoshi Co.,Ltd. 1 kit $600

Description ER-Protein Capture Kit enables to purify ER associated proteins to evaluate various ER roles with easy procedures and no special equipment such as ultracentrifuge machine.
Storage -20°C CAS
Link

ER-Protein Capture Kit | Funakoshi
Lipid Peroxidation Detection Tools for Cancer Research

[Date : June 20 2024 00:07]

ER-Protein Capture Kit


  • Product Code: FDV-0039
  • Supplier: FNA
  • Size: 1kit
  • Price: $600

Description ER-Protein Capture Kit enables to purify ER associated proteins to evaluate various ER roles with easy procedures and no special equipment such as ultracentrifuge machine.
Storage -20°C CAS
Link

ER-Protein Capture Kit | Funakoshi
Lipid Peroxidation Detection Tools for Cancer Research




Go back to index

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