Endoplasmic Reticulum (ER) staining dye for long-term live imaging ERseeing | Funakoshi
Date:July 27 2020Web Page No:81514
Funakoshi Co.,Ltd.
ERseeing is irreversible ER (Endoplasmic Reticulum) staining dye and suitable for long-term live imaging.
Compared to conventional ER staining dye such as Glibenclamide-based dye, ERseeing exhibits very low pharmacological effect and high retentivity which enables to visualize ER even after washout or medium change.
- Webinar
- Background
- ERseeing
- Comparison between ERseeing and conventional ER dye
- Absorption and fluorescent spectrum of ERseeing
- Product information
- Related Product
- You may also like
Webinar
Background
Endoplasmic reticulum (ER) is the largest organelle in a cell and has unique and dynamic tubular or sheet structures.
ER plays essential roles in protein synthesis and carbohydrate metabolism, and is a traffic origin of secreted pathway proteins including the Golgi apparatus, exocytosis, plasma membrane and extracellular proteins.
The most conventional ER dye is based on Glibenclamide fluorophore conjugate.
Glibenclamide is known as a potent and specific inhibitor of the sulphonylurea receptors of ATP sensitive K+ channels.
However, this type of dye has problems as below.
-Negative pharmacological effect on K+ channel in ER.
-Washed out by wash step and medium change.
ERseeing
ERseeing solves the above problems; it exhibits little effect on the ER functions pharmacologically, and enables to visualize ER even after washout or medium change.
ERseeing has two units, a rhodol type green fluorescent dye and a thioester type protein labeling group.
Because this a rhodol type dye has a high affinity to ER membranes, right after adding the reagent to cells, it selectively and quickly accumulates into ER.
Once it accumulated in ER, the labeling group of ERseeing reacts with amino acids in ER-proteins randomly, and forms a covalent bond.
Comparison between ERseeing and conventional ER dye
Comparison of ER specificity
HeLa cells were stained with ERseeing (100 nM) and Glibencramide type ER staining.
ERseeing was highly overlapped with conventional Glibencramide type ER staining dye.
Comparison of retentivety
HeLa cells were treated with ERseeing or Glibenclamide-based dye for 15 min and observed without wash (Left).
Both reagents show ER staining. After that, cells were washed by PBS, added fresh media containing 10% FBS and observed again.
While the Glibenclamide-based dye showed a very weak signal from the cells, ERseeing maintains a good signal from ER.
ERseeing is suitable for long term imaging after medium change.
Absorption and fluorescent spectrum of ERseeing
Excitation (blue) and fluorescent (red) spectrum.
Exmax /Emmax = 509/524 nm.
Commercial FITC filter sets are compatible.
Product information
[Date : February 15 2024 12:37]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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ERseeing, Endoplasmic Reticulum Green DatasheetThis may not be the latest data sheet. |
FDV-0038 | FNAFunakoshi Co.,Ltd. | 10 nmol | $380 | |||||||||||||||||||||||||||||||
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[Date : February 15 2024 12:37]
ERseeing, Endoplasmic Reticulum Green
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0038
- Supplier: FNA
- Size: 10nmol
- Price: $380
Description |
ERseeing is irreversible ER labeling dye and suitable for long-term live imaging. |
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Storage | -20°C | CAS | |
Link |
"ERseeing" (Endoplasmic Reticulum Green Dye) in a minute! |
Related Product
Endoplasmic Reticulum (ER) proteins purification / isolation kit : ER-Protein Capture Kit
[Date : February 15 2024 12:37]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ER-Protein Capture Kit DatasheetThis may not be the latest data sheet. |
FDV-0039 | FNAFunakoshi Co.,Ltd. | 1 kit | $600 | |||||||||||||||||||||||||||||||
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[Date : February 15 2024 12:37]
ER-Protein Capture Kit
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0039
- Supplier: FNA
- Size: 1kit
- Price: $600
Description |
ER-Protein Capture Kit enables to purify ER associated proteins to evaluate various ER roles with easy procedures and no special equipment such as ultracentrifuge machine. |
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Storage | -20°C | CAS | |
Link |
ER-Protein Capture Kit | Funakoshi |
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