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Isolation of phosphorylated peptides: Titansphere series

Date:April 20 2020Web Page No:81463

Titansphere series enables an effective purification and concentration of phosphorylated peptides.

Background

The study of reversible phosphorylation plays an important role to reveal various mechanisms such as apoptosis, cell cycle and so on.
However, some cases show that the detection of phosphorylated peptide is difficult to detect by mass spectrometry due to its low ionization efficiency and low amount.
To solve these issues, Titansphere series was developed using titania dioxide to absorb phosphorylated peptides specifically.
By using Titansphere series, highly purified and concentrated phosphorylated peptide can be obtained.

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Titansphere series

Titansphere Phos-TiO kit (Tip Type)

Easy purification and concentration of phosphorylated peptides by centrifuge.

Phos TiO Kit Box
Phos TiO Kit  how to use

Features

  • Highly purified and concentrated phosphorylated peptides can be obtained.
  • All steps are done easily by centrifugation, reduce human error.
  • 40 minutes to complete all steps.
  • Column materials are porous particles with spherical shape with a wide surface area.
  • Various Applications :Titansphere specifically adsorbs phosphate sites, it can be applicable to the purification of phosphorylated peptides, phosphorylated sugars, glyphosate, etc

Experimental data

Phosphorylated angiotensin II was selectively extracted from mixture of angiotensin II and phosphorylated angiotensin II in only 40 minutes. The recovery rate was 81.6±1.3%.

Phos TiO Kit Experimental data

[Date : May 30 2024 00:08]

Detail Product Name Product Code Supplier Size Price

[Date : May 30 2024 00:08]

*Note: The centrifuge adapter is required, sold separately.

Titansphere Phos-TiO for Large Volume Samples (Cartridge type)

Cartridge type is also available for larger volume samples compared to tip type.
Easy purification and concentration of phosphorylated peptides by centrifuge.

Titansphere Phos Tio Large colum Titansphere Phos Tio Large colum>

[Date : May 30 2024 00:08]

Detail Product Name Product Code Supplier Size Price
Titansphere Phos-TiO 50mg/3mL
DatasheetThis may not be the latest data sheet.
501021290 GLI 25 pieces $1,029

Description
Storage RT CAS
Link

Titansphere Phos-TiO 100mg/3mL
DatasheetThis may not be the latest data sheet.
501021291 GLI 25 pieces $1,669

Description
Storage CAS
Link

[Date : May 30 2024 00:08]

Titansphere Phos-TiO 50mg/3mL


  • Product Code: 501021290
  • Supplier: GLI
  • Size: 25pieces
  • Price: $1,029

Description
Storage RT CAS
Link

Titansphere Phos-TiO 100mg/3mL


  • Product Code: 501021291
  • Supplier: GLI
  • Size: 25pieces
  • Price: $1,669

Description
Storage CAS
Link

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Column material

Titansphere TiO

This material is a porous beads consisting of titania (titanium dioxide) . Posphorylated peptides can be purified and concentrated selectively.

Transphere-TiO-material Transphere titania dioxide column>

Experimental data

Purified efficiency
Using HeLa cell extracts which contain many impurities, the performance of Titansphere TiO was investigated.
The phosphorylated peptides were detected by about 89.9% in the total identified peptide.
Sample amount : 50 µg
Titania amount : 1 mg/ each

Transphere TiO purified efficiency

Performance test compared to IMAC method
Using arabidopsis thaliana cell extracts, the comparison between Titansphere TiO and IMAC was performed.
The phosphorylated peptides using Titansphere TiO was about 2.6 times higher compared to IMAC on total peak area.
Sample: arabidopsis thaliana cell extracts
Sample amount: 100 µg
Titania amount used: 1mg

Transphere TiO performance test

[Date : May 30 2024 00:08]

Detail Product Name Product Code Supplier Size Price
Titansphere TiO 5um
DatasheetThis may not be the latest data sheet.
502075000 GLI 500 mg $480

Description
Storage RT CAS
Link

Titansphere TiO 10um
DatasheetThis may not be the latest data sheet.
502075010 GLI 500 mg $480

Description
Storage RT CAS
Link

Titansphere Phos-TiO Bulk 10um
DatasheetThis may not be the latest data sheet.
501021315 GLI 500 mg $747

Description
Storage RT,Dark Strage CAS
Link

[Date : May 30 2024 00:08]

Titansphere TiO 5um


  • Product Code: 502075000
  • Supplier: GLI
  • Size: 500mg
  • Price: $480

Description
Storage RT CAS
Link

Titansphere TiO 10um


  • Product Code: 502075010
  • Supplier: GLI
  • Size: 500mg
  • Price: $480

Description
Storage RT CAS
Link

Titansphere Phos-TiO Bulk 10um


  • Product Code: 501021315
  • Supplier: GLI
  • Size: 500mg
  • Price: $747

Description
Storage RT,Dark Strage CAS
Link

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Centrifuge Adapter

Usable for Titansphere Phos-TiO Kit and GL-Tip series products.

Phos TiO Centrifuge Adpter

[Date : May 30 2024 00:08]

Detail Product Name Product Code Supplier Size Price
Centrifuge Adaptor for 10µL and 200µL tip
DatasheetThis may not be the latest data sheet.
501021514 GLI 24 pieces $82

Description
Storage CAS
Link

96Well Plate Centrifuge Adaptor for 10µL tip
501021340 GLI 1 piece $116

Description
Storage CAS
Link

96Well Plate Centrifuge Adaptor for 200µL tip
501021341 GLI 1 piece $158

Description
Storage CAS
Link

[Date : May 30 2024 00:08]

Centrifuge Adaptor for 10µL and 200µL tip


  • Product Code: 501021514
  • Supplier: GLI
  • Size: 24pieces
  • Price: $82

Description
Storage CAS
Link

96Well Plate Centrifuge Adaptor for 10µL tip


  • Product Code: 501021340
  • Supplier: GLI
  • Size: 1piece
  • Price: $116

Description
Storage CAS
Link

96Well Plate Centrifuge Adaptor for 200µL tip


  • Product Code: 501021341
  • Supplier: GLI
  • Size: 1piece
  • Price: $158

Description
Storage CAS
Link

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CONTACT

export@funakoshi.co.jp

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  • Please note that Product Information or Price may change without notice.