Reversible Fluorescent Dye for Cell Nucleus NucleoSeeing | Funakoshi
Date:March 12 2019Web Page No:81204
Funakoshi Co.,Ltd.
Reversible Fluorescent Dye for Nucleus
NucleoSeeing
NucleoSeeing is a novel live cell imaging probe which emits green fluorescence by binding to DNA specifically.
NucleoSeeing can be used for not only in animal cells or tissues, but also Guard cell of Arabidopsis thaliana with high S/N ratio. Moreover, NucleoSeeing can be used as a pH sensor in nucleus.
This product has been commercialized under the license from Nagoya Institute of Technology.
- Principle
- Comparison table of dyes for nucleus
- Features
- Example Data
- References
- Webinar Movie
- Product Information
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Principle
NucleoSeeing is composed of green fluorescent dye and DNA binding tag. Under DNA free condition, Nucleoseeing is in folded conformation and no fluorescent is emitted. However, once it binds to DNA, the conformation will change and emitts green fluorescence.
Comparison table of dyes for nucleus
|
Ex/Em |
Fluorescence |
Photo-toxicity |
Nucleus Specificity |
Cell Mem. Permeability |
Cyto-toxicity |
Live |
Fixed |
NucleoSeeing |
488/520 |
Green |
Low |
Yes |
Yes |
Low |
Yes |
Yes |
Hoechst |
350/461 |
Blue |
High |
Yes |
Yes |
High |
Yes |
Yes |
DAPI |
350/461 |
Blue |
High |
Yes |
No |
N/A |
No |
Yes |
Company X |
485/498 |
Green |
Low |
No |
Yes |
N/A |
Yes |
Yes |
Company Y |
646/680 |
Red |
Low |
Yes |
Yes |
High |
Yes |
Yes |
Features
- High S/N ratio
- Excitation / Emission : 488 nm / 520 nm
- Low cytotoxicity
- Can be used for both live and fixed cells
- Can be used for animal samples and plant cells.
(validated in leaf and guard cell of A. thaliana) - Reversible : Can be washed out by replacing the medium
- Can be used as nucleus pH sensor
Example Data
Fig.1 Staining of nucleus in various cultured cells
Six cell lines were treated with 1 μM of NucleoSeeing for 15 min and observed under live condition.
Fig.2 Reversible staining of NucleoSeeing
After HeLa cells were treated 1 μM Hoechst33342 and NucleoSeeing for 15 min, cells were washed by PBS and cultured for 24 hours.
While over 80% of blue signal of Hoechst33342 was still remained, green signals of NucleoSeeing were dramatically reduced within 12 hours.
Fig.3 Staining of Arabidopsis thaliana Guard cells
Arabidopsis thaliana leaves were treated with 20 μM NucleoSeeing in 10 mM MES-KOH, 50 mM KCl (pH 6.2) buffer for 60 min and observed under living condition (Ex. 488 nm and Em. 490-555 nm for NucleoSeeing, Em. >615 nm for chloroplast as autofluorescence).
Green signals from NucleoSeeing were clearly separated from plant autofluorescence derived from chloroplast.
References
1. Nakamura, A., et al., Chem. Commun., 50, 6149-6152 (2014)
Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging.
2. Ueda, M., et al., ACS Cent. Sci., 3 (5), 462-472 (2017)
Noncanonical function of a small-molecular virulence factor coronatine against plant immunity: an in vivo raman imaging approach.
3. Nakamura, A. and Tsukiji, S., Bioorg. Med. Chem. Lett., 27 (14), 3127-3130 (2017)
Ratiometric fluorescence imaging of nuclear pH in living cells using hoechst-tagged fluorescein.
* hoeAc2FL in above 3 papers is equal to NucleoSeeing.
Webinar Movie
Product Information
[Date : February 15 2024 12:37]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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NucleoSeeing, Live Nucleus Green DatasheetThis may not be the latest data sheet. |
FDV-0029 | FNAFunakoshi Co.,Ltd. | 0.1 mg | $350 | |||||||||||||||||||||||||||||||
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[Date : February 15 2024 12:37]
NucleoSeeing, Live Nucleus Green
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0029
- Supplier: FNA
- Size: 0.1mg
- Price: $350
Description |
NucleoSeeing is a novel live cell imaging probe which emits green fluorescence by binding to DNA specifically. NucleoSeeing can be used for not only in animal cells or tissues, but also Guard cell of Arabidopsis thaliana with high S/N ratio. Moreover, NucleoSeeing can be used as a pH sensor in nucleus. |
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Storage | -20°C | CAS | |
Link |
"NucleoSeeing" (Reversible Fluorescent Dye for Nucleus) in a minute! |
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CONTACT
export@funakoshi.co.jp
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