HOME> Products> Cell Culture> Imaging dye> Let's go "Cyto-Seeing" - Reversible cytoplasm blue fluorescent dye : CytoSeeing | Funakoshi

Live Cell Fluorescent Cytoplasm Dye : CytoSeeing Let's go "Cyto-Seeing" - Reversible cytoplasm blue fluorescent dye : CytoSeeing | Funakoshi

Date:March 14 2018Web Page No:81015

Funakoshi Co.,Ltd.

Reversible cytoplasm blue fluorescent dye
CytoSeeing

CytoSeeing is a newly developed fluorescent dye, which can stain cytoplasm promptly by just adding to culture medium. After observation, fluorescent dye can be easily removed by replacing medium which does not contain CytoSeeing.

This product is commercialized based on the research result of Hokkaido University Faculty of Science.

Background

It is well known that morpholgy change of nuclear and cytoplasm are related to differentiation, function and signal response of cells. Conventional cytoplasm staining dyes are used for cell tracking.
Therefore, once dyes are incorporated into cells, the dyes remain in cytoplasm even after medium change.
The dyes are diluted in the course of cell division, and disappear after 3 to 6 population doubling.
If dyes remained in the cells, it is difficult to observe the cell with other probes after live cell imaging.

CytoSeeing overcomes this conventional problem. CytoSeeing can be washed out by just replacing medium without containing CytoSeeing.

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Features

  • Easy protocol
    - Just add CytoSeeing to culture medium.
  • No staining on nucleus
  • Compatible with green and red fluorescent dyes
  • Little effect to cell function
  • Removable
    - Just replace with fresh medium without containing CytoSeeing. Cells can be used for further assays.
  • Compatible with both adhesive and suspension cells.

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Comparison table

Reagent Staining Cytoplasm Check nuclear morphology Time of introduction Wash-out
CytoSeeing Yes Yes 3 - 9 minutes Yes
Company A Yes No 15 - 60 minutes No
Company B Yes No 15 - 60 minutes No

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Product Information

  • Molecular Formula : C17H12N3
  • Molecular Weight : 258.1
  • Purity : >97%
  • No effect to cell function
  • Ex/Em :345 nm / 456 nm
  • Solubility : DMSO (*1)

*1 : 10 mM stock solution is recommended.

spectra

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Protocol Overview

  1. Culture cells
  2. Add CytoSeeing solution to medium, with the final concentration from 10 μM to 50 μM.
  3. Incubate for a few minutes.
  4. Observe cells by fluorescent microscopy.
  5. If needed, remove CytoSeeing by replacing with fresh medium (without containing CytoSeeing).

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Example Data

Fig. 1 Fluorescence imageng of CHO cells with CytoSeeing
CHO cells were treated with 10μM CytoSeeing.


Fig. 2 Incorporation and distribution of CytoSeeing in A549 cells by time course

Fig. 2 Incorporation and distribution of CytoSeeing in A549 cells by time course
a) CytoSeeing (10 μM) was added and incubated. CytoSeeing was completely incorporated into cell by 9 minutes.
b) CytoSeeing incorporated cells were washed and incubated in fresh medium (without CytoSeeing).
Almost CytoSeeing was removed in 30 minutes.


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Reference

Kamada, et al., PLOS ONE, 11:e0160625(2016).

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Webinar Movie

[Date : August 13 2022 00:32]

Detail Product Name Product Code Supplier Size Price
CytoSeeing, Reversible Cytoplasm Blue
DatasheetThis may not be the latest data sheet.
FDV-0017 FNAFunakoshi Co.,Ltd. 1 mg $300

Description CytoSeeing is a newly developed fluorescent dye, which can stain cytoplasm promptly by just adding to culture medium. After observation, fluorescent dye can be easily removed by replacing medium which does not contain CytoSeeing.
Storage -20°C CAS
Link

"CytoSeeing" (Reversible cytoplasm blue fluorescent dye) in a minute!

[Date : August 13 2022 00:32]

CytoSeeing, Reversible Cytoplasm Blue


  • Product Code: FDV-0017
  • Supplier: FNA
  • Size: 1mg
  • Price: $300

Description CytoSeeing is a newly developed fluorescent dye, which can stain cytoplasm promptly by just adding to culture medium. After observation, fluorescent dye can be easily removed by replacing medium which does not contain CytoSeeing.
Storage -20°C CAS
Link

"CytoSeeing" (Reversible cytoplasm blue fluorescent dye) in a minute!

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CONTACT

export@funakoshi.co.jp

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  • Please note that Product Information or Price may change without notice.