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Reversible Fluorescent Dye for Cell Nucleus : NucleoSeeing

Date:March 12 2019Web Page No:81204

Funakoshi Co.,Ltd.

NucleoSeeing logo

Reversible Fluorescent Dye for Nucleus
NucleoSeeing

NucleoSeeing is a novel live cell imaging probe which emits green fluorescence by binding to DNA specifically.
NucleoSeeing can be used for not only in animal cells or tissues, but also Guard cell of Arabidopsis thaliana with high S/N ratio. Moreover, NucleoSeeing can be used as a pH sensor in nucleus.

This product has been commercialized under the license from Nagoya Institute of Technology.

Principle

NucleoSeeing is composed of green fluorescent dye and DNA binding tag. Under DNA free condition, Nucleoseeing is in folded conformation and no fluorescent is emitted. However, once it binds to DNA, the conformation will change and emitts green fluorescence.

NucleoSeeing principle

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Comparison table of dyes for nucleus

 

Ex/Em

Fluorescence

Photo-toxicity

Nucleus Specificity

Cell Mem. Permeability

Cyto-toxicity

Live

Fixed

NucleoSeeing

488/520

Green

Low

Yes

Yes

Low

Yes

Yes

Hoechst

350/461

Blue

High

Yes

Yes

High

Yes

Yes

DAPI

350/461

Blue

High

Yes

No

N/A

No

Yes

Company X

485/498

Green

Low

No

Yes

N/A

Yes

Yes

Company Y

646/680

Red

Low

Yes

Yes

High

Yes

Yes

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Features

  • High S/N ratio
  • Excitation / Emission : 488 nm / 520 nm
  • Low cytotoxicity
  • Can be used for both live and fixed cells
  • Can be used for animal samples and plant cells.
    (validated in leaf and guard cell of A. thaliana)
  • Reversible : Can be washed out by replacing the medium
  • Can be used as nucleus pH sensor

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Example Data

Fig.1 Staining of nucleus in various cultured cells

Fig.1 Staining of nucleus in various cultured cells
Six cell lines were treated with 1 μM of NucleoSeeing for 15 min and observed under live condition.

Fig.2 Reversible staining of NucleoSeeing

Fig.2 Reversible staining of NucleoSeeing
After HeLa cells were treated 1 μM Hoechst33342 and NucleoSeeing for 15 min, cells were washed by PBS and cultured for 24 hours.
While over 80% of blue signal of Hoechst33342 was still remained, green signals of NucleoSeeing were dramatically reduced within 12 hours.

Fig.3 Staining of Arabidopsis thaliana Guard cells

Fig.3 Staining of Arabidopsis thaliana Guard cells
Arabidopsis thaliana leaves were treated with 20 μM NucleoSeeing in 10 mM MES-KOH, 50 mM KCl (pH 6.2) buffer for 60 min and observed under living condition (Ex. 488 nm and Em. 490-555 nm for NucleoSeeing, Em. >615 nm for chloroplast as autofluorescence).
Green signals from NucleoSeeing were clearly separated from plant autofluorescence derived from chloroplast.



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References

3. Nakamura, A. and Tsukiji, S., Bioorg. Med. Chem. Lett., 27 (14), 3127-3130 (2017)
Ratiometric fluorescence imaging of nuclear pH in living cells using hoechst-tagged fluorescein.

* hoeAc2FL in above 3 papers is equal to NucleoSeeing.

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Price

[Date : January 25 2020 00:22]

Detail Product Name Product Code Supplier Size Price
NucleoSeeing <Live Nucleus Green>
DatasheetThis may not be the latest data sheet.
FDV-0029 FNAFunakoshi Co.,Ltd. 0.1 mg $300

Description NucleoSeeing is a novel live cell imaging probe which emits green fluorescence by binding to DNA specifically. NucleoSeeing can be used for not only in animal cells or tissues, but also Guard cell of Arabidopsis thaliana with high S/N ratio. Moreover, NucleoSeeing can be used as a pH sensor in nucleus.
Storage -20°C CAS
Link

[Date : January 25 2020 00:22]

NucleoSeeing <Live Nucleus Green>


  • Product Code: FDV-0029
  • Supplier: FNA
  • Size: 0.1mg
  • Price: $300

Description NucleoSeeing is a novel live cell imaging probe which emits green fluorescence by binding to DNA specifically. NucleoSeeing can be used for not only in animal cells or tissues, but also Guard cell of Arabidopsis thaliana with high S/N ratio. Moreover, NucleoSeeing can be used as a pH sensor in nucleus.
Storage -20°C CAS
Link

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CONTACT

export@funakoshi.co.jp

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