Type A gamma aminobutyric acid receptor (GABAA) Receptor live cell imaging reagent / dye LiveReceptor GABA_AR | Funakoshi

Specific labeling regent for ion channel type GABA receptor
Live Receptor GABA_AR

LiveReceptor is the reagent series of target-specific receptor labeling.
Now, we announce the new product targeting for ion channel type GABA receptors (GABA_ARs)
LiveReceptor GABA_AR is a powerful tool to investigate physiological functions of GABA_AR by monitoring receptor trafficking of GABA_AR in live cells. No complicated protocol is required.

Our "LiveReceptors" generally have three domains, a receptor specific ligand, fluorescein dye and acyl imidazole.
Once ligand on the LiveReceptor binds to the target receptor, acyl imidazole is activated and attacked by nucleophilic amino acids on the receptors' surface.
In this result, the receptors are directly labeled with fluorescein even after the ligand left from binding pocket. Receptor functions are still kept after labeling.

This product is commercialized by the research result of Professor Hamachi, at Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University.

Fig.1 Live cell imaging of labelled GABAARs in GABA_AR-expressed HEK293 GABAAR (α1/β3/γ2)-expressed HEK293 cells were treated with 1 μM of LiveReceptor GABA_AR in the absence or presence of 100 μM gabazine, a GABA_AR selective inhibitor, for three hours at 37C and washed out three times with the basal medium. (scalebars, 20 μm)

Fig.2 Validation of GABA_AR labeling by western blotting GABA_AR (α1/β3/γ2)-expressed HEK293 cells were treated with 0.01-1 μM of LiveReceptor GABA_AR in the absence or presence of 100 μM gabazine, a GABA_AR selective inhibitor,for three hours at 37C. After wash cells, the cells were lysed and proteins were applied into SDS-PAGE and western blotting using anti-fluorescein (FL) antibody or anti-GABAARα1 subunit.

Please down load the movie from here.

LiveReceptor AMPAR