HOME> Products> Cell Culture> Imaging dye> LiveReceptor GABAAR | Funakoshi

Type A gamma aminobutyric acid receptor (GABAA) Receptor live cell imaging reagent / dye LiveReceptor GABAAR | Funakoshi

Date:March 01 2019Web Page No:81197

Funakoshi Co.,Ltd.

LiveReceptor GABAAR logo

Specific labeling regent for ion channel type GABA receptor
Live Receptor GABAAR

LiveReceptor is the reagent series of target-specific receptor labeling.
Now, we announce the new product targeting for ion channel type GABA receptors (GABAARs)
LiveReceptor GABAAR is a powerful tool to investigate physiological functions of GABAAR by monitoring receptor trafficking of GABAAR in live cells. No complicated protocol is required.

LiveReceptor GABA<sub>A</sub>R Principle

Our "LiveReceptors" generally have three domains, a receptor specific ligand, fluorescein dye and acyl imidazole.
Once ligand on the LiveReceptor binds to the target receptor, acyl imidazole is activated and attacked by nucleophilic amino acids on the receptors' surface.
In this result, the receptors are directly labeled with fluorescein even after the ligand left from binding pocket. Receptor functions are still kept after labeling.

This product is commercialized by the research result of Professor Hamachi, at Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University.

Features

  • Can label fluorecein to GABAAR
  • Can observe signal for 1 to 4 hours after adding LiveReceptor to medium.
  • No cell permiability - only GABAAR on cell surface can be labeled.
  • No fluorescence of internalized GABAAR. Only GABAAR on cell surface can be observed.
  • Ion channel function is mainteined after labeling

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Chemical Information

  • Formulation : C55H51N7O13
  • Molecular weight : 1017.35 g/mol
  • Visibility : Orange lyophilized powder
  • Solubility : Soluble in DMSO
    *This compound has high water-solubility, and it can be easily degraded in water and culture medium.
    Please avoid storing in water.
  • Excitation/ Emission: 495/515 nm *Compatible with FITC filter

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Application

  • Live cell imaging
  • Immunocytochemistry with specific antibodies
  • Immunoprecipitation with anti-fluorescein antibody
  • Immunoblotting with anti-fluorescein antibody
  • Drug screening for competitive GABAAR antagonist (GABA-site)

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Example Data

LiveReceptor GABA<sub>A</sub>R Example Data1

Fig.1 Live cell imaging of labelled GABAARs in GABAAR-expressed HEK293 GABAAR (α1/β3/γ2)-expressed HEK293 cells were treated with 1 μM of LiveReceptor GABAAR in the absence or presence of 100 μM gabazine, a GABAAR selective inhibitor, for three hours at 37C and washed out three times with the basal medium. (scalebars, 20 μm)

LiveReceptor GABA<sub>A</sub>R Example Data2

Fig.2 Validation of GABAAR labeling by western blotting GABAAR (α1/β3/γ2)-expressed HEK293 cells were treated with 0.01-1 μM of LiveReceptor GABAAR in the absence or presence of 100 μM gabazine, a GABAAR selective inhibitor,for three hours at 37C. After wash cells, the cells were lysed and proteins were applied into SDS-PAGE and western blotting using anti-fluorescein (FL) antibody or anti-GABAARα1 subunit.

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Webinar Movie

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Price

[Date : July 02 2022 00:30]

Detail Product Name Product Code Supplier Size Price
LiveReceptor GABAAR, GABAAR Labeling Reagent
DatasheetThis may not be the latest data sheet.
FDV-0018B FNAFunakoshi Co.,Ltd. 10 µg $600

Description LiveReceptor GABAAR is a powerful tool to investigate physiological functions of GABAAR by monitoring receptor trafficking of GABAAR in live cells.
Storage -20°C CAS
Link

"LiveReceptor" in a minute!

[Date : July 02 2022 00:30]

LiveReceptor GABAAR, GABAAR Labeling Reagent


  • Product Code: FDV-0018B
  • Supplier: FNA
  • Size: 10µg
  • Price: $600

Description LiveReceptor GABAAR is a powerful tool to investigate physiological functions of GABAAR by monitoring receptor trafficking of GABAAR in live cells.
Storage -20°C CAS
Link

"LiveReceptor" in a minute!



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Related Product

[Date : July 02 2022 00:30]

Detail Product Name Product Code Supplier Size Price
LiveReceptor AMPAR, Endogenous AMPAR Labeling Reagent
DatasheetThis may not be the latest data sheet.
FDV-0018A FNAFunakoshi Co.,Ltd. 10 µg $600

Description LiveReceptor AMPAR is the world-first reagent which can specifically label AMPA-type glutamate receptors (AMPARs) with fluorescein dye on living neurons.
Storage -20°C CAS
Link

LiveReceptor AMPAR | Funakoshi
"LiveReceptor" in a minute!

[Date : July 02 2022 00:30]

LiveReceptor AMPAR, Endogenous AMPAR Labeling Reagent


  • Product Code: FDV-0018A
  • Supplier: FNA
  • Size: 10µg
  • Price: $600

Description LiveReceptor AMPAR is the world-first reagent which can specifically label AMPA-type glutamate receptors (AMPARs) with fluorescein dye on living neurons.
Storage -20°C CAS
Link

LiveReceptor AMPAR | Funakoshi
"LiveReceptor" in a minute!

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CONTACT

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