Powerful but gentle cell dissociation solution Accumax

• Features of Accumax
• Overview of Protocol for Increasing Reproducibility of Cell Counting
• Tested Cell Lines of Accumax
• Price of Accumax
• Related Product : Accutase

Features of Accumax

• Natural enzyme mixture with proteolytic and collagenolytic enzyme activity
• Works gentlely and promptly
• Dissociates tissue
• Increases accuracy of manual or automated cell counts
• Used to dissolve neuronal and prostate spheroids
• Removes cells from 3-D matrixes
• Removes cells from hollow fiber cell reactors
• Extends the sort time of clumpy cell on a sorting flow cytometer
• Non-mammalian and Non-bacterial components contained
• Ready to Use format (prepared by Dulbecco's PBS)
• No need to be neutralized when passaging adherent cells

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Overview of Protocol for Increasing Reproducibility of Cell Counting

1. Harvest a representative sample of clumped cells to be counted, 0.5 or 1.0 ml, and place in a 12 x 75 mm tube.
2. Add an equal volume of Accumax to the sample of cells, and incubate for 5 to 10 minutes at room temperature.
3. Count the cells by your normal procedure.
   * Note that the cells have been diluted an extra 2 fold.

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Tested Cell Lines of Accumax

hESCs	vascular smooth muscle cells	primary chick embryo neuronal cells	HeLa	NT2
bone marrow stem cells	vascular endothelial cells	immortalized mouse testicular germ cells	293	COS
hepatocyte progenitors	hepatocytes	M24 and A375 metastatic melanoma	MG63	Sf9
fibroblasts	macrophages	gliomas U251 and D54	3T3	Vero
keratinocytes	HT1080 fibrosarcoma cells	adherent CHO and BHK cells	L929

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Price of Accumax

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Related Product : Accutase

	Accutase	Accumax
Enzyme Activity	Normal	Strong
Reaction Stopping / Neutralizing	Not Required	Not Required
Animal and Bacterial Component	Not Contained	Not Contained
Phenol Red	Contained	Not Contained

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