Ready to use and suitable replacement of trypsin Accutase®
Date：March 08 2016Web Page No：80531
Funakoshi Co.,Ltd.
Accutase® is a natural enzyme mixture for detachment of adherent cells or dissociation of primary cells from tissues. Accutase® can be used for most cells, even human mesenchymal stem cell or human neural stem cell.
- Features of Accutase
- Overview of Protocol of Accutase
- Tested Cell Lines of Accutase®
- Price of Accutase
- Related Product : Accumax
Features of Accutase
- Natural enzyme mixture with proteolytic and collagenolytic enzyme activity
- Works gently and promptly
- Mimics the action of trypsin and collagenase at the same time.
- High cell viability
- Non-mammalian and Non-bacterial components included.
- Ready to Use format (prepared by Dulbecco's PBS with 0.5 mM EDTA・4Na and phenol red).
- No need to be neutralized when passaging adherent cells.
Fig.1 Human MG63 Fibrosarcoma cells cultured in DMEM + 10% FBS were treated with Accutase. Cell viability was measured by each different treated time. Treatment resulted in rapid cell detachment, a single cell suspension, and high viability. Accutase is gentle on cells; viability was 97 ± 3% even after 45 minutes in Accutase.
Fig.2 Human ES cells are treated with Accutase or Collagenase and cryopreserved. After stored in liquid nitrogen for two months and cultured, total cell numbers and live cells numbers are counted after a day. Accutase treated cells (green) has superior cell viability compared to collagenase treated cells.
Overview of Protocol of Accutase
- Carefully aspirate the medium from cultured dish/well/flask.
- Add enough amount of Accutase to cover the cells.
- Incubate for 5 to 10 minutes at room temperature. (up to a maximum of 1 hour)
- Check the cells frequently to see if they have rounded and no longer attached to the bottom of dish/well/flask.
- Count the cells and passage. No need to add enzyme inhibitors or rinse.
Tested Cell Lines of Accutase®
| hESCs | vascular smooth muscle cells | primary chick embryo neuronal cells | HeLa | NT2 |
| bone marrow stem cells | vascular endothelial cells | immortalized mouse testicular germ cells | 293 | COS |
| hepatocyte progenitors | hepatocytes | M24 and A375 metastatic melanoma | MG63 | Sf9 |
| fibroblasts | macrophages | gliomas U251 and D54 | 3T3 | Vero |
| keratinocytes | HT1080 fibrosarcoma cells | adherent CHO and BHK cells | L929 |
Price of Accutase
[Date : April 30 2024 00:05]
| Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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DatasheetThis may not be the latest data sheet. |
AT104 | FICFunakoshi Co.,Ltd. | 100 ml | $57 | |||||||||||||||||||||||||||||||
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DatasheetThis may not be the latest data sheet. |
AT104-500 | FICFunakoshi Co.,Ltd. | 500 ml | $223 | |||||||||||||||||||||||||||||||
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[Date : April 30 2024 00:05]
DatasheetThis may not be the latest data sheet.
- Product Code: AT104
- Supplier: FIC
- Size: 100ml
- Price: $57
| Description |
Accutase® is a natural enzyme mixture for detachment of adherent cells or dissociation of primary cells from tissues. Accutase® can be used for most cells, even human mesenchymal stem cell or human neural stem cell. Accutase® is a registered trademark of Innovative Cell Technologies, Inc. |
||
|---|---|---|---|
| Storage | -20°C | CAS | |
| Link |
|
||
DatasheetThis may not be the latest data sheet.
- Product Code: AT104-500
- Supplier: FIC
- Size: 500ml
- Price: $223
| Description |
Accutase® is a natural enzyme mixture for detachment of adherent cells or dissociation of primary cells from tissues. Accutase® can be used for most cells, even human mesenchymal stem cell or human neural stem cell. Accutase® is a registered trademark of Innovative Cell Technologies, Inc. |
||
|---|---|---|---|
| Storage | -20°C | CAS | |
| Link |
|
||
Related Product : Accumax
Difference between Accutase and Accumax
| Accutase | Accumax | |
| Enzyme Activity | Normal | Strong |
| Reaction Stopping / Neutralizing | Not Required | Not Required |
| Animal and Bacterial Component | Not Contained | Not Contained |
| Phenol Red | Contained | Not Contained |
[Date : April 30 2024 00:05]
| Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
DatasheetThis may not be the latest data sheet. |
AT104 | FICFunakoshi Co.,Ltd. | 100 ml | $57 | |||||||||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||||||||||||
|
DatasheetThis may not be the latest data sheet. |
AT104-500 | FICFunakoshi Co.,Ltd. | 500 ml | $223 | |||||||||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||||||||||||
[Date : April 30 2024 00:05]
DatasheetThis may not be the latest data sheet.
- Product Code: AT104
- Supplier: FIC
- Size: 100ml
- Price: $57
| Description |
Accutase® is a natural enzyme mixture for detachment of adherent cells or dissociation of primary cells from tissues. Accutase® can be used for most cells, even human mesenchymal stem cell or human neural stem cell. Accutase® is a registered trademark of Innovative Cell Technologies, Inc. |
||
|---|---|---|---|
| Storage | -20°C | CAS | |
| Link |
|
||
DatasheetThis may not be the latest data sheet.
- Product Code: AT104-500
- Supplier: FIC
- Size: 500ml
- Price: $223
| Description |
Accutase® is a natural enzyme mixture for detachment of adherent cells or dissociation of primary cells from tissues. Accutase® can be used for most cells, even human mesenchymal stem cell or human neural stem cell. Accutase® is a registered trademark of Innovative Cell Technologies, Inc. |
||
|---|---|---|---|
| Storage | -20°C | CAS | |
| Link |
|
||
CONTACT
export@funakoshi.co.jp
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- ※Please note that Product Information or Price may change without notice.