HOME> Products> Miscellaneous> Others> Blocking Solutions for Western Blot and ELISA

b-Block / h-Block / k-Block / c-Block Blocking Solutions for Western Blot and ELISA

Date:February 24 2016Web Page No:80489

Finding the appropriate blocking solution is an important factor to get a best data in Western Blot and ELISA.
Four different types of blocking solutions : c-Block / h-Block / k-Block / b-Block make this works easy.
Moreover, the blocking solutions for ELISA are desinged to long-term storage of pre-coated plate.

c-Block / h-Block / k-Block / b-Block

Features

  • Ready-to-Use
  • Optimized blocking solution for Western Blot and ELISA
  • Pre-coated ELISA plate can be stored for several months.

  • b-Block : BSA-based blocking solution. Good for various applications.
  • h-Block : Casein-based blocking solution. Good for various applications.
  • k-Block : Degraded casein-based blocking solution. It shows consistent performance in various applications.
  • c-Block : 100% chemically defined components. Protein free. Best for Western Blot and antibody-detecting ELISA.

  • Trial set : Composed of all the above four solutions. Good for screening of most suitable blocking solution in your work.

Go back to index

Recommendations for screening best blocking solution

For Western Blot

  • To get higher signal (accept some background increase) : k-Block or c-Block
  • To reduce background (accept some decrease of signal) : b-Block or h-Block
  • Using as a standard blocking : h-Block or b-Block
  • Detecting a phospho-protein : c-Block or b-Block

For ELISA

  • Antigen-detecting ELISA - Direct method : c-Block , b-Block or k-Block
    - Sandwich method : k-Block , b-Block or h-Block
  • Antibody-detecting ELISA
    - Direct method (detect antibody captured by pre-coated antigen) : c-Block , b-Block or k-Block
    - Sandwich method : k-Block , b-Block or h-Block

Go back to index

Data

Comparison of blocking solutions

Fig. 1 Comparison of blocking solutions M : Marker / SM : 5% Skim Milk / kB : k-Block / hB : h-Block / bB : b-Block / cB : c-Block


Sandwich ELISA Data

Fig. 2 Sandwich ELISA Data


Stability data of pre-coated plate

Fig. 3 Stability data of pre-coated plate


Go back to index

Price

For Western Blot

[Date : February 15 2024 12:37]

Detail Product Name Product Code Supplier Size Price
b-Block-w
DatasheetThis may not be the latest data sheet.
BCL-BKBW-01 BEABeacle, Inc. 500 ml $130

Description Blocking Solution. b-Block:BSA-based. Good for many applications.
Storage 4°C CAS
Link

h-Block-w
DatasheetThis may not be the latest data sheet.
BCL-BKHW-01 BEABeacle, Inc. 500 ml $100

Description Blocking Solution. h-Block:Casein-based. Good for many applications.
Storage 4°C CAS
Link

k-Block-w
DatasheetThis may not be the latest data sheet.
BCL-BKKW-01 BEABeacle, Inc. 500 ml $100

Description Blocking Solution. k-Block:degnatured casein-based. Shows stable results in many applications.
Storage 4°C CAS
Link

c-Block-W
DatasheetThis may not be the latest data sheet.
BCL-BKCW-01 BEABeacle, Inc. 500 ml $100

Description Blocking agent. 100% chemical components. Good for WB and antibody-detecting ELISA.
Storage 4°C CAS
Link

Blocking Solution Trial Set(Western) 4x20ml each
DatasheetThis may not be the latest data sheet.
BCL-BKSW-01 BEABeacle, Inc. 1 set $60

Description Blocking Solutions have following main ingredients and characteristics ; c-Block:100% chemical components. Good for WB and antibody-detecting ELISA. h-Block:Casein-based. Good for many applications. k-Block:degnatured casein-based. Shows stable results in many applications. b-Block:BSA-based. Good for many applications. Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution
Storage 4°C CAS
Link

[Date : February 15 2024 12:37]

b-Block-w


  • Product Code: BCL-BKBW-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $130

Description Blocking Solution. b-Block:BSA-based. Good for many applications.
Storage 4°C CAS
Link

h-Block-w


  • Product Code: BCL-BKHW-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $100

Description Blocking Solution. h-Block:Casein-based. Good for many applications.
Storage 4°C CAS
Link

k-Block-w


  • Product Code: BCL-BKKW-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $100

Description Blocking Solution. k-Block:degnatured casein-based. Shows stable results in many applications.
Storage 4°C CAS
Link

c-Block-W


  • Product Code: BCL-BKCW-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $100

Description Blocking agent. 100% chemical components. Good for WB and antibody-detecting ELISA.
Storage 4°C CAS
Link

Blocking Solution Trial Set(Western) 4x20ml each


  • Product Code: BCL-BKSW-01
  • Supplier: BEA
  • Size: 1set
  • Price: $60

Description Blocking Solutions have following main ingredients and characteristics ; c-Block:100% chemical components. Good for WB and antibody-detecting ELISA. h-Block:Casein-based. Good for many applications. k-Block:degnatured casein-based. Shows stable results in many applications. b-Block:BSA-based. Good for many applications. Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution
Storage 4°C CAS
Link

For ELISA

[Date : February 15 2024 12:37]

Detail Product Name Product Code Supplier Size Price
b-Block-e
DatasheetThis may not be the latest data sheet.
BCL-BKBE-01 BEABeacle, Inc. 500 ml $150

Description Blocking Solution. b-Block:BSA-based. Good for many applications.
Storage 4°C CAS
Link

h-Block-e
DatasheetThis may not be the latest data sheet.
BCL-BKHE-01 BEABeacle, Inc. 500 ml $125

Description Blocking Solution. h-Block:Casein-based. Good for many applications.
Storage CAS
Link

k-Block-e
DatasheetThis may not be the latest data sheet.
BCL-BKKE-01 BEABeacle, Inc. 500 ml $125

Description Blocking Solution. k-Block:degnatured casein-based. Shows stable results in many applications.
Storage 4°C CAS
Link

c-Block-e
DatasheetThis may not be the latest data sheet.
BCL-BKCE-01 BEABeacle, Inc. 500 ml $125

Description Blocking Solutions. c-Block:100% chemical components. Good for WB and antibody-detecting ELISA.
Storage 4°C CAS
Link

Blocking Solution Trial Set(ELISA) 4x20ml Each
DatasheetThis may not be the latest data sheet.
BCL-BKSE-01 BEABeacle, Inc. 1 set $70

Description Blocking Solutions have following main ingredients and characteristics ; c-Block:100% chemical components. Good for WB and antibody-detecting ELISA. h-Block:Casein-based. Good for many applications. k-Block:degnatured casein-based. Shows stable results in many applications. b-Block:BSA-based. Good for many applications. Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution
Storage 4°C CAS
Link

[Date : February 15 2024 12:37]

b-Block-e


  • Product Code: BCL-BKBE-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $150

Description Blocking Solution. b-Block:BSA-based. Good for many applications.
Storage 4°C CAS
Link

h-Block-e


  • Product Code: BCL-BKHE-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $125

Description Blocking Solution. h-Block:Casein-based. Good for many applications.
Storage CAS
Link

k-Block-e


  • Product Code: BCL-BKKE-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $125

Description Blocking Solution. k-Block:degnatured casein-based. Shows stable results in many applications.
Storage 4°C CAS
Link

c-Block-e


  • Product Code: BCL-BKCE-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $125

Description Blocking Solutions. c-Block:100% chemical components. Good for WB and antibody-detecting ELISA.
Storage 4°C CAS
Link

Blocking Solution Trial Set(ELISA) 4x20ml Each


  • Product Code: BCL-BKSE-01
  • Supplier: BEA
  • Size: 1set
  • Price: $70

Description Blocking Solutions have following main ingredients and characteristics ; c-Block:100% chemical components. Good for WB and antibody-detecting ELISA. h-Block:Casein-based. Good for many applications. k-Block:degnatured casein-based. Shows stable results in many applications. b-Block:BSA-based. Good for many applications. Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution
Storage 4°C CAS
Link

Go back to index

CONTACT

export@funakoshi.co.jp

  • Prices are for USA / Canada customers. Prices do not include shipping and handling charges, VAT, import tariffs and service charge etc.
  • Please note that Product Information or Price may change without notice.