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b-Block / h-Block / k-Block / c-Block Blocking Solutions for Western Blot and ELISA

Date：February 24 2016Web Page No：80489

Beacle, Inc.

Finding the appropriate blocking solution is an important factor to get a best data in Western Blot and ELISA.
Four different types of blocking solutions : c-Block / h-Block / k-Block / b-Block make this works easy.
Moreover, the blocking solutions for ELISA are desinged to long-term storage of pre-coated plate.

c-Block / h-Block / k-Block / b-Block

Features

  • Ready-to-Use
  • Optimized blocking solution for Western Blot and ELISA
  • Pre-coated ELISA plate can be stored for several months.

  • b-Block : BSA-based blocking solution. Good for various applications.
  • h-Block : Casein-based blocking solution. Good for various applications.
  • k-Block : Degraded casein-based blocking solution. It shows consistent performance in various applications.
  • c-Block : 100% chemically defined components. Protein free. Best for Western Blot and antibody-detecting ELISA.

  • Trial set : Composed of all the above four solutions. Good for screening of most suitable blocking solution in your work.

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Recommendations for screening best blocking solution

For Western Blot

  • To get higher signal (accept some background increase) : k-Block or c-Block
  • To reduce background (accept some decrease of signal) : b-Block or h-Block
  • Using as a standard blocking : h-Block or b-Block
  • Detecting a phospho-protein : c-Block or b-Block

For ELISA

  • Antigen-detecting ELISA - Direct method : c-Block , b-Block or k-Block
    - Sandwich method : k-Block , b-Block or h-Block
  • Antibody-detecting ELISA
    - Direct method (detect antibody captured by pre-coated antigen) : c-Block , b-Block or k-Block
    - Sandwich method : k-Block , b-Block or h-Block

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Data

Comparison of blocking solutions

Fig. 1 Comparison of blocking solutions M : Marker / SM : 5% Skim Milk / kB : k-Block / hB : h-Block / bB : b-Block / cB : c-Block


Sandwich ELISA Data

Fig. 2 Sandwich ELISA Data


Stability data of pre-coated plate

Fig. 3 Stability data of pre-coated plate


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Price

For Western Blot

[Date : April 25 2025 00:06]

Detail Product Name Product Code Supplier Size Price
b-Block-w
DatasheetThis may not be the latest data sheet. 		BCL-BKBW-01 BEABeacle, Inc. 500 ml $118

Description Blocking Solution. b-Block:BSA-based. Good for many applications.
Storage 4°C CAS
Link

h-Block-w
DatasheetThis may not be the latest data sheet. 		BCL-BKHW-01 BEABeacle, Inc. 500 ml $91

Description Blocking Solution. h-Block:Casein-based. Good for many applications.
Storage 4°C CAS
Link

k-Block-w
DatasheetThis may not be the latest data sheet. 		BCL-BKKW-01 BEABeacle, Inc. 500 ml $91

Description Blocking Solution. k-Block:degnatured casein-based. Shows stable results in many applications.
Storage 4°C CAS
Link

c-Block-w
DatasheetThis may not be the latest data sheet. 		BCL-BKCW-01 BEABeacle, Inc. 500 ml $91

Description Blocking agent. 100% chemical components. Good for WB and antibody-detecting ELISA.
Storage 4°C CAS
Link

Blocking Solution Trial Set(Western) 4x20ml each
DatasheetThis may not be the latest data sheet. 		BCL-BKSW-01 BEABeacle, Inc. 1 set $54

Description Blocking Solutions have following main ingredients and characteristics ; c-Block:100% chemical components. Good for WB and antibody-detecting ELISA. h-Block:Casein-based. Good for many applications. k-Block:degnatured casein-based. Shows stable results in many applications. b-Block:BSA-based. Good for many applications. Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution
Storage 4°C CAS
Link

[Date : April 25 2025 00:06]

b-Block-w

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKBW-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $118

Description Blocking Solution. b-Block:BSA-based. Good for many applications.
Storage 4°C CAS
Link

h-Block-w

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKHW-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $91

Description Blocking Solution. h-Block:Casein-based. Good for many applications.
Storage 4°C CAS
Link

k-Block-w

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKKW-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $91

Description Blocking Solution. k-Block:degnatured casein-based. Shows stable results in many applications.
Storage 4°C CAS
Link

c-Block-w

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKCW-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $91

Description Blocking agent. 100% chemical components. Good for WB and antibody-detecting ELISA.
Storage 4°C CAS
Link

Blocking Solution Trial Set(Western) 4x20ml each

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKSW-01
  • Supplier: BEA
  • Size: 1set
  • Price: $54

Description Blocking Solutions have following main ingredients and characteristics ; c-Block:100% chemical components. Good for WB and antibody-detecting ELISA. h-Block:Casein-based. Good for many applications. k-Block:degnatured casein-based. Shows stable results in many applications. b-Block:BSA-based. Good for many applications. Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution
Storage 4°C CAS
Link

For ELISA

[Date : April 25 2025 00:06]

Detail Product Name Product Code Supplier Size Price
b-Block-e
DatasheetThis may not be the latest data sheet. 		BCL-BKBE-01 BEABeacle, Inc. 500 ml $136

Description Blocking Solution. b-Block:BSA-based. Good for many applications.
Storage 4°C CAS
Link

h-Block-e
DatasheetThis may not be the latest data sheet. 		BCL-BKHE-01 BEABeacle, Inc. 500 ml $113

Description Blocking Solution. h-Block:Casein-based. Good for many applications.
Storage CAS
Link

k-Block-e
DatasheetThis may not be the latest data sheet. 		BCL-BKKE-01 BEABeacle, Inc. 500 ml $113

Description Blocking Solution. k-Block:degnatured casein-based. Shows stable results in many applications.
Storage 4°C CAS
Link

c-Block-e
DatasheetThis may not be the latest data sheet. 		BCL-BKCE-01 BEABeacle, Inc. 500 ml $113

Description Blocking Solutions. c-Block:100% chemical components. Good for WB and antibody-detecting ELISA.
Storage 4°C CAS
Link

Blocking Solution Trial Set(ELISA) 4x20ml Each
DatasheetThis may not be the latest data sheet. 		BCL-BKSE-01 BEABeacle, Inc. 1 set $64

Description Blocking Solutions have following main ingredients and characteristics ; c-Block:100% chemical components. Good for WB and antibody-detecting ELISA. h-Block:Casein-based. Good for many applications. k-Block:degnatured casein-based. Shows stable results in many applications. b-Block:BSA-based. Good for many applications. Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution
Storage 4°C CAS
Link

[Date : April 25 2025 00:06]

b-Block-e

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKBE-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $136

Description Blocking Solution. b-Block:BSA-based. Good for many applications.
Storage 4°C CAS
Link

h-Block-e

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKHE-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $113

Description Blocking Solution. h-Block:Casein-based. Good for many applications.
Storage CAS
Link

k-Block-e

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKKE-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $113

Description Blocking Solution. k-Block:degnatured casein-based. Shows stable results in many applications.
Storage 4°C CAS
Link

c-Block-e

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKCE-01
  • Supplier: BEA
  • Size: 500ml
  • Price: $113

Description Blocking Solutions. c-Block:100% chemical components. Good for WB and antibody-detecting ELISA.
Storage 4°C CAS
Link

Blocking Solution Trial Set(ELISA) 4x20ml Each

DatasheetThis may not be the latest data sheet.


  • Product Code: BCL-BKSE-01
  • Supplier: BEA
  • Size: 1set
  • Price: $64

Description Blocking Solutions have following main ingredients and characteristics ; c-Block:100% chemical components. Good for WB and antibody-detecting ELISA. h-Block:Casein-based. Good for many applications. k-Block:degnatured casein-based. Shows stable results in many applications. b-Block:BSA-based. Good for many applications. Trial set:a trial kit composed of all the above 4 solutions. Good for screening of best blocking solution
Storage 4°C CAS
Link

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