HOME> Products> Miscellaneous> Signal Booster

Signal Enhancer for Western Blot & ELISA Signal Booster

Date:March 23 2017Web Page No:46147

Need higher signals? Try Signal Booster Neo or Signal Booster!

Signal Booster : Signal Enhancer for western blot and ELISA

Antigen-antibody reaction enhancing reagents for western blotting, ELISA, etc. By simply diluting antibodies with Signal Boosters, signals increase and background decrease.

For immunostaining, please check Signal Booster Immunostain.

 Wellcome bulk purchase 

Features

  • Possible to obtain 2-10 times higher signals comparing to conventional methods using surfactant-containing buffers.
  • Widely used in various immunoassay systems involving antigen-antibody reaction such as western blotting and ELISA.
  • HRP or ALP will not be affected by Signal Booster. Luminecence or fluorescence detection system is also compatible.
  • Useful for saving antibody and shortening reaction time.
  • Can be stored in a refrigerator for more than 1 year.

Signal Booster Neo

  • One solution system
  • Protein-free. Chemically defined
  • For phospho protein detection or for any ELISA: Protein-free composition contributes to no interference by proteins in the reagent.

Signal Booster

  • Two components system. For primary antibody, dilute by solution A. For secondary antibody, dilute by solution B.
  • It contains protein.

Go back to index

Principle

The main composition of Signal Booster series are polymer and blocking ingredients. Polymer alters the surface physico-chemical properties of antigens and antibodies. This makes antigen and antibody enhance the contact frequency and specific binding. Combination of polymer and blocking ingredients helps you to get higher signal with lower background.


Go back to index

Example of Use

Signal Booster Neo

Signal Booster : Signal Enhancer for Western Blot and ELISA

Detection of phosphoprotein (phospho-EGFR)


SB: Signal Booster, SBN: Signal Booster Neo
Sample: A431 cell lysate, Blocking: c-Block (cat#BCL-BKCW-01)
1st Ab: anti-pEGFR, rabbit, 2nd Ab: anti-rabbit, goat polyclonal, HRP


Signal Booster : Signal Enhancer for Western Blot and ELISA

Detection of HBs antigen by ELISA


Blocking: b-block (cat#BCL-BKBE-01)
Capture Ab: anti-HBsAg mouse, Detection Ab: anti-mouse IgG-HRP


Signal Booster : Signal Enhancer for Western Blot and ELISA

Detection of mouse serum amyloid A3 by western blot


Mouse serum amyloid A3, overexpressed in E.Coli 1st and 2nd antibodies are diluted in TBST-dried milk or Signal Booster Neo according to manufacturer’s instructions. There was a dramatic difference with the Signal Booster Neo.
(Data provided by Dr. Robert Anderberg, Providence Medical Research Center, Providence Health Care)

Signal Booster

Signal Booster : Signal Enhancer for Western Blot and ELISA

Detection of luciferase


NT: TBS-T, SB: Signal Booster, A: company A
Sample: luciferase expressing HepG2cell lysate
1st Ab: anti-luciferase IgG, goat polyclonal, 1/1000 diluted by solution A
2nd Ab: anti-goat IgG, donkey HRP-labeled, 1/20000 diluted by solution B

When Signal Booster was used, the signal intensity was stronger than that of TBS-T. In addition, the signal intensity was equal to or higher than that of company A.

Go back to index

Product Information

Signal Booster Neo

[Date : April 25 2025 00:06]

Detail Product Name Product Code Supplier Size Price
Signal Booster Neo
DatasheetThis may not be the latest data sheet.
BCL-SBN-01 BEABeacle, Inc. 250 ml $163

Description Signal Booster Neo is a newly developed protein-free signal enhancer and good for to detect phospho proteins or in conditions where protein interfer the assays. 1.One solution system 2.Stronger signal and lower background as compared to ordinary method 3.No interference by proeins for phospho protein detection or for any ELISA due to protein-free 4.Wide variety of applications 5.Easy to use
Storage 4°C CAS
Link

Signal Booster Neo
DatasheetThis may not be the latest data sheet.
BCL-SBN-02 BEABeacle, Inc. 500 ml $290

Description A newly developed protein-free signal enhancer and good for to detect phospho proteins or in conditions where protein interfer the assays.
Storage 4°C CAS
Link

[Date : April 25 2025 00:06]

Signal Booster Neo


  • Product Code: BCL-SBN-01
  • Supplier: BEA
  • Size: 250ml
  • Price: $163

Description Signal Booster Neo is a newly developed protein-free signal enhancer and good for to detect phospho proteins or in conditions where protein interfer the assays. 1.One solution system 2.Stronger signal and lower background as compared to ordinary method 3.No interference by proeins for phospho protein detection or for any ELISA due to protein-free 4.Wide variety of applications 5.Easy to use
Storage 4°C CAS
Link

Signal Booster Neo


  • Product Code: BCL-SBN-02
  • Supplier: BEA
  • Size: 500ml
  • Price: $290

Description A newly developed protein-free signal enhancer and good for to detect phospho proteins or in conditions where protein interfer the assays.
Storage 4°C CAS
Link


Signal Booster

[Date : April 25 2025 00:06]

Detail Product Name Product Code Supplier Size Price
Signal Booster Solution A&B set 50mL each
DatasheetThis may not be the latest data sheet.
BCL-105 BEABeacle, Inc. 1 set $73

Description Signal Boosteris the signal enhancing cocktail. By simply dilute antibodies with Signal Booster, you can get stronger signal while reducing background. The reagent can be used for the variety of immunoreaction-related detection systems. Signal Booster is consisted of two types of cocktail solutions, Solution A and Solution B. Please refer to Manual for the usage since the two solutions differ in their composition and the nature of enhancement.
Storage 4°C CAS
Link

Signal Booster Solution A&B set 100mL each
DatasheetThis may not be the latest data sheet.
BCL-110 BEABeacle, Inc. 1 set $118

Description Signal Boosteris the signal enhancing cocktail. By simply dilute antibodies with Signal Booster, you can get stronger signal while reducing background. The reagent can be used for the variety of immunoreaction-related detection systems. Signal Booster is consisted of two types of cocktail solutions, Solution A and Solution B. Please refer to Manual for the usage since the two solutions differ in their composition and the nature of enhancement.
Storage 4°C CAS
Link

Signal Booster Solution A&B set 250mL each
DatasheetThis may not be the latest data sheet.
BCL-125 BEABeacle, Inc. 1 set $209

Description Signal enhancing cocktail. By simply dilute antibodies, you can get stronger signal while reducing background.
Storage 4°C CAS
Link

[Date : April 25 2025 00:06]

Signal Booster Solution A&B set 50mL each


  • Product Code: BCL-105
  • Supplier: BEA
  • Size: 1set
  • Price: $73

Description Signal Boosteris the signal enhancing cocktail. By simply dilute antibodies with Signal Booster, you can get stronger signal while reducing background. The reagent can be used for the variety of immunoreaction-related detection systems. Signal Booster is consisted of two types of cocktail solutions, Solution A and Solution B. Please refer to Manual for the usage since the two solutions differ in their composition and the nature of enhancement.
Storage 4°C CAS
Link

Signal Booster Solution A&B set 100mL each


  • Product Code: BCL-110
  • Supplier: BEA
  • Size: 1set
  • Price: $118

Description Signal Boosteris the signal enhancing cocktail. By simply dilute antibodies with Signal Booster, you can get stronger signal while reducing background. The reagent can be used for the variety of immunoreaction-related detection systems. Signal Booster is consisted of two types of cocktail solutions, Solution A and Solution B. Please refer to Manual for the usage since the two solutions differ in their composition and the nature of enhancement.
Storage 4°C CAS
Link

Signal Booster Solution A&B set 250mL each


  • Product Code: BCL-125
  • Supplier: BEA
  • Size: 1set
  • Price: $209

Description Signal enhancing cocktail. By simply dilute antibodies, you can get stronger signal while reducing background.
Storage 4°C CAS
Link


Just a Solution A or Solution B

[Date : April 25 2025 00:06]

Detail Product Name Product Code Supplier Size Price
Signal Booster Solution A
DatasheetThis may not be the latest data sheet.
BCL-110A BEABeacle, Inc. 100 ml $73

Description Signal Booster is a standard signal enhancer and shows stable enhancing activity in many kind of assays. Solution A : the dilution of the 1st antibody.
Storage 4°C CAS
Link

Signal Booster Solution A
DatasheetThis may not be the latest data sheet.
BCL-125A BEABeacle, Inc. 250 ml $118

Description Signal Booster is a standard signal enhancer and shows stable enhancing activity in many kind of assays. Solution A : the dilution of the 1st antibody.
Storage 4°C CAS
Link

Signal Booster Solution B
DatasheetThis may not be the latest data sheet.
BCL-125B BEABeacle, Inc. 250 ml $118

Description Signal Booster is a standard signal enhancer and shows stable enhancing activity in many kind of assays. Signal Booster Solution B: Reaction solution for the secondary antibody.
Storage 4°C CAS
Link

[Date : April 25 2025 00:06]

Signal Booster Solution A


  • Product Code: BCL-110A
  • Supplier: BEA
  • Size: 100ml
  • Price: $73

Description Signal Booster is a standard signal enhancer and shows stable enhancing activity in many kind of assays. Solution A : the dilution of the 1st antibody.
Storage 4°C CAS
Link

Signal Booster Solution A


  • Product Code: BCL-125A
  • Supplier: BEA
  • Size: 250ml
  • Price: $118

Description Signal Booster is a standard signal enhancer and shows stable enhancing activity in many kind of assays. Solution A : the dilution of the 1st antibody.
Storage 4°C CAS
Link

Signal Booster Solution B


  • Product Code: BCL-125B
  • Supplier: BEA
  • Size: 250ml
  • Price: $118

Description Signal Booster is a standard signal enhancer and shows stable enhancing activity in many kind of assays. Signal Booster Solution B: Reaction solution for the secondary antibody.
Storage 4°C CAS
Link

Go back to index

CONTACT

export@funakoshi.co.jp

  • Prices on our website are for your reference only. Please inquire your distributor for your prices.
  • Please note that Product Information or Price may change without notice.