Fluorescence probe that can measure the temperature inside a cell by fluorescence lifetime Cellular Thermoprobe™ for Fluorescence Lifetime
Date：March 11 2015Web Page No：80404
Funakoshi Co.,Ltd.
Cellular Thermoprobe™ for Fluorescence Lifetime is a fluorescence probe that can measure temperature inside a cell using fluorescence lifetime microscopy system. It can be introduced into cells by treating cells with a 5% glucose solution containing the fluorescent probe.
※ Learn more about ☞ Diffusive Thermoprobe™, which introduces fluorescent probes into cells by microinjection.
※ Learn more about ☞ Cellular Thermoprobe™ for Fluorescence Ratio, which measures temperature inside cells by fluorescence ratio.
- Cell Function & Temperature
- Features
- Structure & Principles
- Outline of Protocol
- Fluorescence Response & Temperature Resolution in HeLa cells
- Introduction for HeLa cells
- Cited by
- Video
- Product Information
Cell Function & Temperature
Temperature is physical quantities that controls all chemical reactions in cells. Chemical reactions involving exothermic or endothermic reactions at a specific site in cells to carry out cell-function induce local temperature-change. Therefore, the intracellular temperature distribution reflects the thermodynamics and function of intracellular molecules. From the medical perspective, it has been reported that pathological cells such as cancer cells have enhanced thermogenesis. Therefore, understanding the intracellular temperature distribution is expected to contribute to a better understanding of cellular functions and to the development of new diagnostic and therapeutic methods.
Features
- It can be introduced into cells by treating cells with a mixture of 5% glucose solution.
- It can be introduced into both adherent and suspension cells by incubating cells with Thermoprobe™ solution.
- It can be introduced into cells in a 10 minute reaction.
- The temperature detection range is between 28-38℃, and can detect temperature differences as small as 0.05-0.54℃.
- Spatial resolution of fluorescence lifetime microscope is 200 nm, allowing detection of temperature differences inside a cell.
- Using a plate reader equipped with fluorescence lifetime meter or fluorescence lifetime measurement function, temperature measurement can also be performed on a cell population.
- Temperature measurements with Cellular Thermoprobe™ for Fluorescence Lifetime are negligibly affected by intracellular ionic strength, pH, protein content and viscosity.
- Since measurement is based on fluorescence lifetime, temperature can be accurately measured without being affected by local concentration of fluorescent probe, excitation light intensity, fading, excitation wavelength, etc.
- Solubility: Soluble in water
- Appearance: Yellow powder
※ Fluorescence lifetime imaging system is required separately.
Structure & Principles
Cellular Thermoprobe™ for Fluorescence Ratio is composed of thermosensitive unit (NNPAM), cation unit (APTMA) and fluorescent unit 1 (DBThD-AA) . When a probe solution is at low temperature, fluorescent is weak due to the presence of water molecules in the structure. However, at high temperature, the hydrophobic interaction of thermosensitive unit causes the probe to become small and rounded, so that water molecules are excluded from the probe and strong fluorescence is emitted. In addition, the incorporation of cation unit allows to be spontaneously introduced into cells in a short time.
Outline of Protocol
- Add 20 μl of ultrapure water to 200 μg powder and dissolve.
- Dilute with 5% glucose solution*.
- Add Cellular Thermoprobe™ for Fluorescence Ratio in 5 % glucose solution* to cells.
- Incubate the cells at 25℃ for 10 min.
- Wash the cells with PBS and add culture medium.
- Observe with a fluorescence lifetime microscopy systema having a cage incubation chamber.
＊ In the case that the dissociation of the adherent cells were observed in 5% glucose solution, 5% glucose solution with 0.1-0.3 mM CaCl2 may improve it.
Fluorescence Response & Temperature Resolution in HeLa cells
Introduction for HeLa cells
Cited by
- Kato H., et. al., Life Sci. Alliance, 3 (3), e201900576 (2020). [PMID:32029570]
- Inada N., et. al., Nat. Protoc., 14(4): 1293-1321 (2019). doi:10. 1038/s41596-019-0145-7 [PMID:30903107]
- Hoshi Y., et. al., J. Neurosci., 38(25), 5700～5709 (2018). [PMID:29793978]
- Hayashi T., et. al., PLoS ONE., 10(2), e0117677 (2015). [PMID:25692871]
Video
Please down load the movie from here.
Product Information
[Date : April 30 2025 00:06]
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Cellular Thermoprobe for Fluorescence Lifetime DatasheetThis may not be the latest data sheet. |
FDV-0004 | FNAFunakoshi Co.,Ltd. | 200 µg | $120 | |||||||||||||||||||||||||||||||
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Cellular Thermoprobe for Fluorescence Lifetime DatasheetThis may not be the latest data sheet. |
FDV-0004 | FNAFunakoshi Co.,Ltd. | 3x200 µg | $320 | |||||||||||||||||||||||||||||||
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[Date : April 30 2025 00:06]
Cellular Thermoprobe for Fluorescence Lifetime
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0004
- Supplier: FNA
- Size: 200µg
- Price: $120
| Description |
“Cellular Thermoprobe for Fluorescence Lifetime” is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence lifetime imaging microscopy. It can be delivered into cell without microinjection. It is highly photostable and easy-to-use. With its cell permeability, it is applicable for both adherent and suspension cells. It enables us to distinguish intracellular temperature in cultured mammalian cells at organelle level. License:This product is licensed by Tokyo Universityand KIRIN Co., Ltd. |
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|---|---|---|---|
| Storage | RT,Dark Strage | CAS | |
| Link |
|
||
Cellular Thermoprobe for Fluorescence Lifetime
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0004
- Supplier: FNA
- Size: 3x200µg
- Price: $320
| Description |
“Cellular Thermoprobe for Fluorescence Lifetime” is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence lifetime imaging microscopy. It can be delivered into cell without microinjection. It is highly photostable and easy-to-use. With its cell permeability, it is applicable for both adherent and suspension cells. It enables us to distinguish intracellular temperature in cultured mammalian cells at organelle level. License:This product is licensed by Tokyo Universityand KIRIN Co., Ltd. |
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|---|---|---|---|
| Storage | RT,Dark Strage | CAS | |
| Link |
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CONTACT
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