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HOME> Products> Cell Culture> Imaging dye> Diffusive Thermoprobe^™

Fluorescence probe for intracellular temperature distribution imaging Diffusive Thermoprobe^™

Date：September 08 2014Web Page No：46141

Funakoshi Co.,Ltd.

Diffusive Thermoprobe^™ is a fluorescent probe that can be introduced into cells by microinjection and used with a fluorescence lifetime microscopy system to measure the temperature of intracellular organelles.
※ Learn more about ☞ Particulate Thermoprobe^™, fluorescent probe for measuring cell temperature.
※ Learn more about ☞ Cellular Thermoprobe^™ for Fluorescence Ratio, which measures temperature inside cells by fluorescence ratio.

ATP synthesis inhibitor (FCCP) increases temperature in surrounding mitochondria in COS7 cells — ATP synthesis inhibitor increases temperature in surrounding mitochondria in COS7 cells

Green : Diffusive Thermoprobe^™
Red : Mitochondria

Left : Confocal fluorescence image
Mid : Fluorescence lifetime image
Right : Fluorescence lifetime image after addition of ATP synthesis inhibitor FCCP

The average temperature difference before and after addition of FCCP was 1.02±0.17℃.

☞ Product Information

Cell Function & Temperature
Features
Structure & Principles
Outline of Protocol
Example Data
Cited by
Product Information

Cell Function & Temperature

Temperature is physical quantities that controls all chemical reactions in cells. Chemical reactions involving exothermic or endothermic reactions at a specific site in cells to carry out cell-function induce local temperature-change. Therefore, the intracellular temperature distribution reflects the thermodynamics and function of intracellular molecules. From the medical perspective, it has been reported that pathological cells such as cancer cells have enhanced thermogenesis. Therefore, understanding the intracellular temperature distribution is expected to contribute to a better understanding of cellular functions and to the development of new diagnostic and therapeutic methods.

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Features

Temperature detection limit is 0.18-0.5℃, which enables detection of even the smallest temperature differences.
Spatial resolution is 200 nm and can detect temperature differences in intracellular organelles.
Temperature measurements with Diffusive Thermoprobe^™ are negligibly affected by intracellular ionic strength, pH, protein content and viscosity.
Since measurement is performed by fluorescence lifetime, temperature can be accurately measured without being affected by local concentration of fluorescent probe, excitation light intensity, fading, excitation wavelength, etc.
Solubility: Soluble in water
Appearance: Yellow powder

※ A microinjection system and fluorescence lifetime imaging system are required separately.

☞ Product Information

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Structure & Principles

Diffusive Thermoprobe^™ is composed of thermosensitive unit (NNPAM), hydrophilic unit (SPA) and fluorescent unit (DBD-AA) . When a probe solution is at low temperature, fluorescent is weak due to the presence of water molecules in the structure. However, at high temperature, the hydrophobic interaction of thermosensitive unit causes the probe to become small and rounded, so that water molecules are excluded from the probe and strong fluorescence is emitted. In addition, the incorporation of hydrophilic unit prevents aggregation of fluorescent temperature sensors within a cell, enabling temperature distribution measurements that require high spatial resolution.

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Outline of Protocol

Reconstitute 100 μg powder at 10 μl of 80 mM KCl, 10 mM K₂HPO₄, 4 mM NaCl.
Leave it at +4℃ at least 8 hours.
Take 1 μl solution from vial, and fill it up to glass capillary needle for microinjection.
Microinject it into cytoplasm with a glass capillary needle below 30℃.
Leave cells for 30min.
Analyze intracellular temperature by fluorescence lifetime imaging microscopy.

☞ Product Information

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Example Data

Left: Confocal fluorescence image
Right: fluorescence lifetime image

Temperature mapping near mitochondria in COS7 cells

Green : Diffusive Thermoprobe^™
Red : Mitochondria

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Cited by

Okabe, K., et. al., Nat. Commun., 28(3), 705(2012).

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Product Information

[Date : April 25 2025 00:06]

Detail Product Name Product Code Supplier Size Price

Diffusive Thermoprobe
DatasheetThis may not be the latest data sheet. FDV-0002 FNAFunakoshi Co.,Ltd. 100 µg $260

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Description	Diffusive Thermoprobe is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence lifetime imaging microscopy. This product is licensed by Tokyo University.
Storage	RT	CAS
Link

[Date : April 25 2025 00:06]

Diffusive Thermoprobe

DatasheetThis may not be the latest data sheet.

Product Code: FDV-0002
Supplier: FNA
Size: 100µg
Price: $260

Description	Diffusive Thermoprobe is a fluorescent polymeric thermometer for living cells. It diffuses throughout the cells and gives the information about intracellular temperature distribution by fluorescence lifetime imaging microscopy. This product is licensed by Tokyo University.
Storage	RT	CAS
Link

Contact

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CONTACT

export@funakoshi.co.jp

※Prices on our website are for your reference only. Please inquire your distributor for your prices.
※Please note that Product Information or Price may change without notice.

Fluorescence probe for intracellular temperature distribution imaging Diffusive Thermoprobe™

Cell Function & Temperature

Features

Structure & Principles

Outline of Protocol

Example Data

Cited by

Product Information

CONTACT

Fluorescence probe for intracellular temperature distribution imaging Diffusive Thermoprobe^™