Fluorescent probe for tracking average temperature in single cell Particulate Thermoprobe™
Date：September 08 2014Web Page No：46138
Funakoshi Co.,Ltd.
Particulate Thermoprobe™ is a fluorescent probe that can measure cell temperature by measuring fluorescence intensity of temperature-dependent aggregation of the probe under a fluorescence microscope after cell introduction by microinjection.
※ Learn more about ☞ Diffusive Thermoprobe™, which introduces fluorescent probes into cells by microinjection.
※ Learn more about ☞ Cellular Thermoprobe™ for Fluorescence Ratio, which measures temperature inside cells by fluorescence ratio.
- Cell Function & Temperature
- Strong Point
- Structure & Principles
- Outline of Protocol
- Temperature-dependent Fluorescence Intensity
- Cited by
- Product Information
Cell Function & Temperature
Temperature is physical quantities that controls all chemical reactions in cells. Chemical reactions involving exothermic or endothermic reactions at a specific site in cells to carry out cell-function induce local temperature-change. Therefore, the intracellular temperature distribution reflects the thermodynamics and function of intracellular molecules. From the medical perspective, it has been reported that pathological cells such as cancer cells have enhanced thermogenesis. Therefore, understanding the intracellular temperature distribution is expected to contribute to a better understanding of cellular functions and to the development of new diagnostic and therapeutic methods.
Strong Point
- Particulate Thermoprobe™ is a nanogel-type fluorescent temperature probe that aggregates in a temperature-dependent manner after being introduced into cells, and its fluorescence intensity depends on temperature. By measuring the fluorescence intensity of Particulate Thermoprobe™ in a single cell, the average temperature of the cell can be calculated.
- Temperature detection limit is 0.5℃ or less, allowing detection of even the slightest temperature difference.
- Particulate Thermoprobe™ is not affected by cellular environmental conditions such as pH, ionic strength, protein content, and viscosity etc.
- Average Diameter : 48 nm
- Solubility: Soluble in water
- Appearance: Yellow powder
※ A microinjection system is required separately.
Structure & Principles
Particulate Thermoprobe™ is composed of thermo-responsive unit (polyNIPAM), water-sensitive fluorescent unit (DBThD-AA) and crosslinking unit (MBAM). When a probe solution is at low temperature, water molecules are absorbed into Particulate Thermoprobe™ and the fluorescence of DBThD-AA inside is quenched by the surrounding water molecules. As temperature rises, Particulate Thermoprobe™ contracts and emits fluorescence as the water molecules are released.
Outline of Protocol
- Reconstitute 100 μg powder at 10 μl of 80 mM KCl, 10 mM K2HPO4, 4 mM NaCl.
- Leave it at +4℃ at least 8 hours.
- Take 1 μl solution from vial, and fill it up to glass capillary needle for microinjection.
- Microinject it into cytoplasm with a glass capillary needle below 30℃.
- Leave cells for 30min.
- Analyze intracellular temperature by fluorescence microscopy.
Temperature-dependent Fluorescence Intensity
Fluorescence Intensity of Particulate Thermoprobe™ in COS7 cell extracts
Fluorescence intensity increased 5.9-fold in COS7 cell extracts when the solution temperature increased from 23℃ to 35℃.
Cited by
- Uchiyama, S., et al., Chem. Eur. J., 18(31), 9552～9563(2012).
- Gota, C., et al., J. Am. Chem. Soc., 131(8), 2766～2767(2009).
Product Information
[Date : April 25 2025 00:06]
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Particulate Thermoprobe DatasheetThis may not be the latest data sheet. |
FDV-0003 | FNAFunakoshi Co.,Ltd. | 100 µg | $260 | |||||||||||||||||||||||||||||||
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[Date : April 25 2025 00:06]
Particulate Thermoprobe
DatasheetThis may not be the latest data sheet.
- Product Code: FDV-0003
- Supplier: FNA
- Size: 100µg
- Price: $260
| Description |
Particulate Thermoprobe is a fluorescent nanogel thermometer for living cells. It aggregates in proportion to increase in the temperature and fluorescence intensity is proportional to its aggregation. This product is licensed by Tokyo University. |
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