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Next generation 2D cell culture substrate that combines the functions of laminin and Perlecan perLAM : Perlecan - Conjugated Laminin E8

Date：August 18 2026Web Page No：718223

Perlecan-conjugated Laminin E8 fragment (perLAM) is a cell culture substrate that mimics the basement membrane in living organisms. perLAM can reproduce the extracellular environment of living organisms in cell culture systems.
Cells understand their environment, recognize their position, and work methodically with other cells around them. In cell culture, biological activities of cells are expected to be different because their environments are significantly different from those in living organisms. perLAM was developed based on the idea that it is important to reproduce the extracellular environment in order to show the same biological activities in cell culture and living organisms. So, the product is expected to contribute implementation of regenerative medicine, animal testing alternatives, etc.

* iMatrix series using Laminin E8 fragment can be found at the link.
* This product is for research use only.


What is Perlecan?

Perlecan is a multifunctional protein found in the basement membrane. It acts as a structural and signaling hub - binding important growth factors and interacting with matrix components to support cell growth, communication, and differentiation. Its unique Heparan Sulfate (HS) chains allow it to capture and stabilize molecules like bEGF, making the surrounding matrix more than just a scaffold - it becomes an active, instructive environment.

What is Perlecan?

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Why use Perlecan-conjugated Laminin E8?

It is known that Heparan sulfate (HS) chain exists at Domain I of Perlecan. By fusing the region with the Laminin E8 fragment, Perlecan-Conjugated Laminin E8 fragment is developed as a next-generation cell culture matrix. This fusion matrix can be expected to induce differentiation induction from pluripotent stem cell cultures with high efficiency.

Perlecan-conjugated Laminin E8 functions

Perlecan-conjugated Laminin E8 functions

Perlecan-conjugated Laminin E8 functions
The fusion of Laminin E8 fragment and Perlecan Domain I is a mimic of basement membrane components, and shows the same functions of it on the basement membrane. The fusion is a cell culture matrix that reproduces the functions of basement membranes.

Comparison of differentiation from hiPS cells to skeletal muscle cells

Comparison of differentiation induction from hiPS cells to skeletal muscle cells
Differentiation efficiencies from hiPS cells were compared among different cell culture matrices (scaffolds). The scaffolds used for the comparison were EHS gel basement membrane matrix, Laminin E8 fragment, and Perlecan-conjugated Lamini E8 fragment. MCH and MYOD1 is used as myocyte markers for the comparison. From the result, it was concluded that the matrix using Perlecan-conjugated Lamini E8 fragment showed the highest differentiation efficiency than the other matrices.



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perLAM-521

*1 Adachi, H., et al., Stem cells translational medicine, 11(7), 767-777(2022). [PMID:35605097]

perLAM-421

*2 Zhao, M., et al., Adv. Sci. (Weinh.)., 11(26), e2308306(2024). [PMID:38685581]


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perLAM-521

perLAM-521 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-521 E8.

perLAM-521

Features

  • Laminin E8 fragment enables stable large scale cell cultures of pluripotent stem cells by binding specifically and strongly to integrin in cells.
  • It can be used as an addition method (adding to cell suspension) that does not require a coating operation.
  • The heparan sulfate chains in Perlecan Domain I efficiently capture growth factors such as bFGF and deliver them to receptors on the cell surface.
  • It enhances the differentiation induction of hiPS cells into the ectoderm or mesoderm.
  • This product, which is cell culture matrix for 2D culture, is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.

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perLAM-421

perLAM-421 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-421 E8.

perLAM-421

Features

  • Laminin E8 fragment enables stable large scale cell cultures of pluripotent stem cells by binding specifically and strongly to integrin in cells.
  • It can be used as an addition method (adding to cell suspension) that does not require a coating operation.
  • The heparan sulfate chains in Perlecan Domain I efficiently capture growth factors such as bFGF and deliver them to receptors on the cell surface.
  • It enhances the differentiation induction of hiPS cells into the skeletal muscle cells.
  • This product, which is cell culture matrix for 2D culture, is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.
Differentiation from hiPS cells to Skeletal muscle cells
MHC-MYOD1-DAPI

Differentiation from hiPS cells to skeletal muscle cells
Sample: Skeletal muscle cells originated from hiPS cells (Day 38 from induction)
The cells induced by Perlecan-conjugated Laminin-421 E8 fragment showed high expression levels of myocyte markers, MHC, and MYOD1.


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Product information

perLAM-521

[Date : August 19 2026 00:08]

Detail Product Name Product Code Supplier Size Price
perLAM-521
DatasheetThis may not be the latest data sheet. 		101521 MAX 2x175 µg $414

Description perLAM-521 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-521 E8. It enhances the differentiation induction of hiPS cells into the ectoderm or mesoderm. This product is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.
Storage -20°C CAS
Link

perLAM-521
DatasheetThis may not be the latest data sheet. 		101522 MAX 6x175 µg $1,160

Description perLAM-521 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-521 E8. It enhances the differentiation induction of hiPS cells into the ectoderm or mesoderm. This product is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.
Storage -20°C CAS
Link

[Date : August 19 2026 00:08]

perLAM-521

DatasheetThis may not be the latest data sheet.


  • Product Code: 101521
  • Supplier: MAX
  • Size: 2x175µg
  • Price: $414

Description perLAM-521 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-521 E8. It enhances the differentiation induction of hiPS cells into the ectoderm or mesoderm. This product is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.
Storage -20°C CAS
Link

perLAM-521

DatasheetThis may not be the latest data sheet.


  • Product Code: 101522
  • Supplier: MAX
  • Size: 6x175µg
  • Price: $1,160

Description perLAM-521 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-521 E8. It enhances the differentiation induction of hiPS cells into the ectoderm or mesoderm. This product is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.
Storage -20°C CAS
Link


perLAM-421

[Date : August 19 2026 00:08]

Detail Product Name Product Code Supplier Size Price
perLAM-421
DatasheetThis may not be the latest data sheet. 		101421 MAX 2x175 µg $456

Description perLAM-421 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-421 E8. It enhances the differentiation induction of hiPS cells into the skeletal muscle cells. This product is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.
Storage -20°C CAS
Link

perLAM-421
DatasheetThis may not be the latest data sheet. 		101422 MAX 6x175 µg $1,243

Description perLAM-421 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-421 E8. It enhances the differentiation induction of hiPS cells into the skeletal muscle cells. This product is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.
Storage -20°C CAS
Link

[Date : August 19 2026 00:08]

perLAM-421

DatasheetThis may not be the latest data sheet.


  • Product Code: 101421
  • Supplier: MAX
  • Size: 2x175µg
  • Price: $456

Description perLAM-421 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-421 E8. It enhances the differentiation induction of hiPS cells into the skeletal muscle cells. This product is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.
Storage -20°C CAS
Link

perLAM-421

DatasheetThis may not be the latest data sheet.


  • Product Code: 101422
  • Supplier: MAX
  • Size: 6x175µg
  • Price: $1,243

Description perLAM-421 is a cell adhesive matrix with conjugation of Perlecan Domain I and Laminin-421 E8. It enhances the differentiation induction of hiPS cells into the skeletal muscle cells. This product is produced as a recombinant protein mixture containing no unknown components and is supplied with consistent performance and quality.
Storage -20°C CAS
Link


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