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Custom oligo synthesis with high specificity, high stability, and low toxicity Custom LNA and BNATM oligonucleotides synthesis service

Date：June 22 2026Web Page No：520120

LNA (Locked Nucleic Acid) and BNATM (Bridged Nucleic Acid) are types of oligonucleotides that have a crosslinking structure in the nucleic acid molecules, which provide excellent stability and specificity for complementary strands. Oligonucleotides with the artificial nucleic acids are ideal tools for detection of small RNA targets, highly similar DNA and RNA targets, and so on.
This custom service offers various scales of DNA/RNA oligonucleotide synthesis using LNA (2', 4'-Bridged Nucleic Acid, 2', 4'-BNATM) with various modifications. In addition, BNATMsNC which is known to provide high stability and high specificity to complementary strands are also available.
Upon request, in vivo grade is available by filter sterilization and lyophilization under sterile condition.

* The service is provided by GeneDesign, Inc., a part of Ajinomoto Bio-Pharma Services.
* Click here for ordering information.
* The service is for research use only.

About BNATM oligonucelotides

BNATM oligonucleotides are manufactured by Ajinomoto Bio-Pharma Services’ GeneDesign facility in Japan for R&D purposes based on a license agreement with RIKEN GENESIS Co., Ltd., in Japan.

Structure of BNATMs

BNAオリゴヌクレオチドについて

* Available BNATMs for this service
   LNA (2', 4'-BNATM) : A, G, T, mC (Methylcytosine)
    BNATM NC : T, mC (Methylcytosine)


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What are BNATM and LNA oligonucleotides?

BNATM is an abbreviated name of Bridged Nucleic Acid and was developed by Dr. Takeshi Imanishi (Professor, Faculty of Pharmaceutical Sciences, Osaka University). Around 10 types of BNATMs have been developed in the past and the individual BNATMs have different properties such as binding affinity (hybridization ability), and nuclease resistance. Since natural nucleic acids such as DNA and RNA have high conformational flexibility, double strand formation (hybridization) between DNA-DNA or RNA-RNA is thermodynamically unfavorable. Therefore, improving the binding affinity of nucleic acids is a major challenge in the field of artificial nucleic acid development. In order to obtain high binding affinity to targeted nucleic acids (DNA and RNA) and additional nuclease resistance by the bridged structure in the nucleic acid molecules, BNATM was designed by a different concept of other conventional artificial nucleic acids which is restriction of the conformational flexibility of the natural nucleic acids (shown as red lines in Figure 1). The artificial nucleic acids are ideal tools for genomic technologies targeting RNA, single-strand DNA, and double-strand DNA such as antisense technology, RNAi, decoy nucleic acid, gene recombination, ribozyme, and DNA enzyme. In recent years, research and development of 2', 4'-BNATM (LNA) oligonucleotide has been conducted to use as common base materials for nucleic acid drugs and diagnostic systems.

Fig. 1 Basic Structures of BNAs

Figure 1 Basic structures of BNATMs


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Features of LNA (2', 4'-BNATM) and BNATM

  • LNA and BNATM have extensively high binding affinity to DNA and RNA because of the fixed N-type structure of it.
  • Oligonucleotides with partial insertion of LNA or BNATM have superior nuclease resistance compared with natural oligonucleotides.
  • Various modifications such as terminal modification, and phosphorothioate formation are compatible.
  • There have been studies suggesting that LNA oligomers have higher safety when compared to S-oligos (Phosphorothioate oligonucleotides) for in vivo studying.

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Application examples of LNA (2', 4'-BNATM)

  • Antisense oligonucleotides instead of S-oligonucleotides1
  • Application to siRNA
  • Functional inhibition of miRNA2
  • in situ hybridization probe etc.

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Nuclease resistance of LNA (2', 4'-BNATM) oligonucleotides

It is suggested that LNA (2', 4'-BNATM) oligonucleotides have improved stability in animals because oligonucleotides with partial insertion of LNA have superior nuclease resistance compared with natural oligonucleotides. In experiments using animal cells and/or laboratory animals, even if a small amount of LNA oligos is expected to produce long-lasting effects such as antisense and siRNA.


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Advantages of in vivo use of LNA (2', 4'-BNATM) oligonucleotides

There have been studies suggesting that LNA oligomers have higher safety when compared to S-oligos. LNA oligomers are conventionally used for antisense oligonucleotides in in vivo experiments. It was reported that rats injected with S-oligo in the brain showed a clear exothermic reaction, and nerve cell dropouts were observed in the brain histopathology of this rat. On the other hand, for rats injected with LNA-oligo, such pathological findings are not seen. As a result, LNA-oligo can be regarded as safer in vivo than S-oligo1.


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Characteristics Table of LNA and BNATM NC

LNA（2', 4'-BNATM）BNATM NCBNATM NC(Me)
Binding Capacity◎◎◎
Enzyme Resistance●◎◎

Advantage: ◎ > ○ > ● > Natural nucleic acid

Basic Property of BNAs

* Additional information of BNATM and LNA below is available. Click here!
  • High binding affinity
  • High nuclease resistance
  • Toxicity study example

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Reference

  1. Wahlestedt, C., et al., Proc. Natl. Acad. Sci. USA, 97, 5633～5638 (2000).
  2. Corsten, M. F., et al., Cancer Res., 67, 8994～9000 (2007).
  3. Obernosterer, G., et al., Nat. Protoc., 2, 1508～1514 (2007).
  4. Mitsuoka、Y.、et al., Nucleic Acids Res., 37, 1225～1238 (2009)．
  5. Abdur Rahman、S. M., et al., J. Am. Chem. Soc., 130, 4886～4896 (2008)．

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How to order

Please download the Excel file of Quotation Request Sheet, fill out the form including oligo sequences and other required information, and send the file to your local distributor. We only accept the Excel file; we do not accept PDF or FAX. After reviewed by GeneDesign, we will send a quote via your local dealer.

* A, G, T, mC (methylated cytosine) can be supplied in LNA (2', 4'-BNATM). U and C are not available.
* Delivery amount is estimated by absorption spectrometry.


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CONTACT

export@funakoshi.co.jp

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