Gentle Culture, Superior Yields: Stress-Free Growth Static Culture Bag OKUDAKE

The OKUDAKE Static Culture Bag is an innovative film bag with ultra-high gas permeability that revolutionizes microbial cultivation. By enabling efficient gas exchange even when completely sealed, OKUDAKE allows aerobic microorganisms to thrive in a static environment. It completely eliminates the need for mechanical shaking or stirring, providing a stress-free culture environment that yields exceptional growth and opens new doors for morphological research.
* In Japanese, "OKUDAKE” means “just place it”.

＊This product is for research use only.

Static culture of E. coli using OKUDAKE demonstrated growth efficiency comparable to conventional shaking culture. Furthermore, when evaluating the production yield of a recombinant enzyme (plant derived peroxidase from Physcomitrium patens), static culture with OKUDAKE achieved higher production efficiency than shaking culture.

Bacillus sp. was cultured under static conditions using OKUDAKE. Under these conditions, the cells successfully formed a biofilm. In contrast, when cultured under conventional shaking conditions, the cells remained dispersed and no biofilm formation was observed.

Aspergillus oryzae was cultured using this product. The culture grown in OKUDAKE exhibited higher growth efficiency than shaking culture in a flask, and the amount of produced substances was more than twice as high. Furthermore, while shaking culture resulted in the formation of spherical pellets, no pellet formation was observed when cultured using this product, indicating a more uniform and favorable growth environment.

Physcomitrium patens cultured in OKUDAKE was observed under an inverted microscope. Due to the high transparency of OKUDAKE, clear microscopic images could be obtained without removing the plant from the bag, enabling seamless, contamination free observation.

Chlamydomonas reinhardtii was cultured in OKUDAKE. The culture grown in OKUDAKE exhibited a higher growth rate than shaking culture in flasks, and the final biomass yield was also greater. These results demonstrate that OKUDAKE is highly suitable for algal culture, offering an efficient and user friendly alternative to traditional shaking methods.

Four tadpole shrimps collected from rice fields were sealed in OKUDAKE or a commercially available polyethylene bag with 500 mL of water. By the second day, all tadpole shrimps sealed in the polyethylene bag had died, whereas all shrimps sealed in OKUDAKE survived.
In addition, dissolved oxygen (DO) levels in OKUDAKE were twice as high as those in the polyethylene bag, demonstrating that the high gas permeability of OKUDAKE supplies sufficient oxygen to the enclosed water and supports organism survival.

* OKUDAKE is supplied nonsterile. If sterilization is required, please autoclave or UV sterilize the bag beforehand.
* If using an impulse sealer, ensure that your device can properly seal OKUDAKE, as sealing performance varies by model.
* Avoid applying pressure to the bag, as this may cause damage or leakage.

Traditional aerobic microbial culture relies heavily on shaking and mechanical agitation to maintain oxygen supply. While effective for aeration, these methods can introduce physical stress that may affect microbial behavior, morphology, and productivity.

OKUDAKE was designed as a new approach to microbial cultivation. Using an ultra gas-permeable film, the bag enables efficient oxygen exchange even during static culture, eliminating the need for mechanical aeration.

The result is a simple, non -shear cultivation environment that supports natural microbial growth, biofilm formation, and recombinant protein production.