Gentle Culture, Superior Results: No Physical Stress! Static Culture Bag OKUDAKE

Static Culture Bag OKUDAKE (OKUDAKE means “Just put it” in Japanese) is a film bag for microbial culture with extremely high gas permeability. It allows sufficient gas exchange even when sealed, enabling static culture of aerobic microorganisms. It eliminates the need for mechanical aeration such as shaking or aerobic stirring, achieving culture without physical stress on microorganisms.

* This product is for research use only.

In liquid microbial cultures, gas exchange via mechanical aeration such as shaking or aerobic stirring is routinely performed to supply oxygen, which is essential for survival and yet poorly soluble in water. However, these mechanical operations constantly subject microorganisms to physical stress. To solve this problem, it is necessary to supply oxygen to the culture without mechanical operation, but no effective method has been available so far. To overcome the problem, the “Static Culture Bag OKUDAKE” is made from a film material with extremely high gas permeability.
It enables sufficient oxygen supply to the culture even during static conditions due to its extremely high gas permeability, achieving microbial culture without physical stress.

The following dimensions are available. Please select the one best suited to your needs. Custom orders are also available.
Please contact us at (export@funakoshi.co.jp) for more detailed information.

* The optimal culture volume varies depending on the culture medium, culture conditions, and purpose.
   Please optimize the conditions according to your experiment.

Static culture of E. coli using OKUDAKE showed equivalent growth efficiency to conventional shaking culture. Furthermore, when measuring the production yield of the recombinant enzyme (plant-derived peroxidase from Physcomitrium patens), static culture using OKUDAKE showed higher production efficiency than shaking culture.

Bacillus sp. was cultured under static conditions using OKUDAKE. As a result, they formed a biofilm, whereas they remained dispersed and no biofilm formation was observed in conventional shaking culture.

Epicoccum nigrum was cultured under static conditions using OKUDAKE. OKUDAKE showed superior growth efficiency compared to shaking culture in a flask. Furthermore, while shaking culture resulted in the formation of spherical pellets, no such pellets were observed in the culture using OKUDAKE.

Physcomitrium patens cultured in OKUDAKE was subjected to microscopic observation under an inverted microscope. Due to the high transparency of OKUDAKE, clear images could be obtained without taking out the plant out of OKUDAKE.

Chlamydomonas reinhardtii was cultured in OKUDAKE. As a result, the culture using OKUDAKE showed a higher growth rate than that of shaking culture using flasks, and the final biomass yield was also greater. This demonstrates that OKUDAKE is also suitable for algal culture.

Four tadpole shrimps collected from rice fields were sealed in OKUDAKE or a commercially available polyethylene bag with 500 ml of water. As a result, on the second day, all tadpole shrimps sealed in the polyethylene bag had died, while all those sealed in OKUDAKE survived. Furthermore, dissolved oxygen (DO) levels were twice as high in OKUDAKE compared to the polyethylene bag, demonstrating that the high gas permeability of the product supplied sufficient oxygen to the enclosed water.