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HOME> Products> Assay Kits> ELISA / EIA kit> PD-L1 TN-cyclon™ ELISA Kit

PD-L1 in exosome can also be analyzed PD-L1 TN-cyclon™ ELISA Kit

Date：February 20 2026Web Page No：160276

This is a high-sensitivity assay kit for human PD-L1 using "improved enzyme cycling method (TN-cyclon™)". This kit does not require special equipment but can quantify extremely small amounts of PD-L1. This feature enables high-sensitivity assay of human PD-L1 in samples containing serum or exosomes.

* If you would like to use TN-cyclon™ technology with your antibodies and antigens, please check here.
* This product is for research use only.

TN-cyclon™ (improved enzyme cycling method)
Ultrasensitive protein detection technology combining sandwich ELISA and enzyme cycling. The substrate reacted by sandwich ELISA becomes the substrate for the next enzyme cycling method, and the reaction is repeated. By measuring Thio-NADH accumulated at this time, it is possible to quantify very small amounts of protein.

What is the relation between PD-L1 and Cancer
Features
Assay Example
Kit Contents
Product Information

What is the relation between PD-L1 and Cancer

PD-L1 is an immunosuppressive protein molecule that inhibits or stops the activity of T cells by binding to its receptor, PD-1. PD-L1 is normally expressed on the surface of antigen-presenting cells, but it is also known to be expressed on the cell surface of tumor cells and non-transformed cells in the tumor microenvironment. It has also been shown that blocking the binding with PD-1 by immune checkpoint inhibitors inhibits the growth of tumor cells, and the expression level of PD-L1 in tumor tissue and the amount of PD-L1 released into the blood are considered as candidates for useful predictive biomarkers.

PD-L1 is also present in serum (soluble PD-L1) and exosomes. In cancer, exosomes are used for horizontal transmission and play an important role in the mechanism of cancer metastasis. In addition, since PD-L1 is also present in the serum of healthy individuals to a small extent, it is important to understand how PD-L1 changes quantitatively during the transition from healthy individuals to cancer patients. These findings will reaffirm the importance of immune checkpoints and contribute to improving the response rate of immune checkpoint inhibitors. However, since the amount of exosomes is small in the first place and PD-L1 is also low in the serum of healthy individuals, ultra-trace measurement of PD-L1 is necessary to distinguish the transition from healthy individuals to early-stage cancer patients.

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Features

It is more than 1 order of magnitude more sensitive than conventional ELISA methods, enabling ultra-sensitive measurement (0.1 pg/ml).
Simple method and no specialized equipment required^*.
Species: Human
Specimen: Serum or exosome-containing samples
Assay format: 96 well plate
Method: Colorimetric
Wavelength: 405 nm (reference wavelength: 660 nm)

* Absorption plate reader is required for measurement.

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Assay Example

Standard Curve

The left graph shows the calibration curve of the standard PD-L1 concentrations (pg/ml) obtained from the absorbance data (405 nm (Δ 0 min)). The right table shows the absorbance and the coefficient of variation (CV) data of each PD-L1 concentration.
Measurement wavelength: 405 nm (primary wavelength), 660 nm (reference wavelength), Measurement time: 60 min

Limit of Detection

The limit of detection, calculated using the standard deviation (SD) of the blank absorbance (3SD applied) and the slope of the calibration curve, was below 0.2 pg/mL (0.154 pg/mL in the example above).

Spike and Recovery Test (PD-L1 in serum)

PD-L1 with a known concentration (100 pg/ml) was added to serum samples and the recovery rate of it was calculated.
* The serum samples were diluted 100 fold before use in Sample Diluent.

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Kit Contents

Reagents

Antibodies (Capture Antibody, Detection Antibody (APL-conjugated))
Standard (Recombinant Protein)
Diluents
Wash Buffer
Blocking Reagent
Enzyme Cycling Reagents (4 types)
Enzyme Cycling Diluents (2 types)

Accessories

96-Well Plate (1 plate)
Adhesive Plate Seals (5 units)
User Manual

* Please refer to the Datasheet for other required materials not supplied with the kit, reagent preparation methods, and assay protocols.

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Product Information

[Date : February 25 2026 00:09]

Detail Product Name Product Code Supplier Size Price

PD-L1 TN-cyclon ELISA Kit
DatasheetThis may not be the latest data sheet. BPMA-TNPDL1-1e BPM 1 kit $2,968

Contact

Description	The measurement of PD-L1 with this kit is based on TN-cyclon, a proprietary technology that combines the sandwich ELISA and enzymatic cycling methods. This method enables highly sensitive quantification of human PD-L1 in serum or exosome samples. No special equipment is required, and the assay can be performed with an ordinary absorption plate reader. Assay samples: Serum, Exosomes. Cross-species: Human. Detection limit: 0.2 pg/ml or less.
Storage	4°C,-20°C,-80°C	CAS
Link	TN-cyclon™ ELISA Development Kit

[Date : February 25 2026 00:09]

PD-L1 TN-cyclon ELISA Kit

DatasheetThis may not be the latest data sheet.

Product Code: BPMA-TNPDL1-1e
Supplier: BPM
Size: 1kit
Price: $2,968

Description	The measurement of PD-L1 with this kit is based on TN-cyclon, a proprietary technology that combines the sandwich ELISA and enzymatic cycling methods. This method enables highly sensitive quantification of human PD-L1 in serum or exosome samples. No special equipment is required, and the assay can be performed with an ordinary absorption plate reader. Assay samples: Serum, Exosomes. Cross-species: Human. Detection limit: 0.2 pg/ml or less.
Storage	4°C,-20°C,-80°C	CAS
Link	TN-cyclon™ ELISA Development Kit

Contact

Related Product

TN-cyclon ELISA Development Kit Contents

TN-cyclon™ ELISA Development Kit is for developing a detection kit of an ultra-low-abundant protein with a combination of your matched-pair antibodies and a corresponding antigen by TN-cyclon™ (improved enzymatic cycling method).

[Date : February 25 2026 00:09]

Detail Product Name Product Code Supplier Size Price

TN-cyclon ELISA Development Kit
DatasheetThis may not be the latest data sheet. BPMA-TNEDVA-1e BPM 1 kit $2,002

Contact

Description	You can construct a detection system for low-abundance proteins by this TN-cyclon technology using the antibodies and antigens you have. It does not require special equipment and can be measured with an ordinary absorption plate reader. *High sensitivity measurement cannot always be guaranteed depending on the combination of antibodies and antigens, antibody reactivity, epitope relationship, etc.
Storage	4°C,-20°C	CAS
Link	TN-cyclon™ ELISA Development Kit

[Date : February 25 2026 00:09]

TN-cyclon ELISA Development Kit

DatasheetThis may not be the latest data sheet.

Product Code: BPMA-TNEDVA-1e
Supplier: BPM
Size: 1kit
Price: $2,002

Description	You can construct a detection system for low-abundance proteins by this TN-cyclon technology using the antibodies and antigens you have. It does not require special equipment and can be measured with an ordinary absorption plate reader. *High sensitivity measurement cannot always be guaranteed depending on the combination of antibodies and antigens, antibody reactivity, epitope relationship, etc.
Storage	4°C,-20°C	CAS
Link	TN-cyclon™ ELISA Development Kit

Contact

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