Keyword	：

Keyword	：
Supplier	：Select	Product code	：
Product type	：	Discontinued product	：

Keyword	：
Supplier	：Select	Product code	：
Discontinued product	：

AntigenHelp	：	Cross reactivityHelp	：	Host animalHelp	：
ConjugationHelp	：	ApplicationHelp	：	PurificationHelp	：
AbsorptionHelp	：	IsotypeHelp	：	CloneHelp	：

Product type	：	Match condition	：	Discontinued product	：

Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：
Supplier	：Select	Product code	：

CAS No	：

Supplier	：Select	Discontinued product	：

WEB page No search	：

HOME> Products> Assay Kits> ELISA / EIA kit> TN-cyclon™ ELISA Development Kit

Create your own quantitative kit for a low-abundance protein with your on-hand antibodies! TN-cyclon™ ELISA Development Kit

Date：February 17 2026Web Page No：520122

This kit is for developing a detection kit of an ultra-low-abundant protein with a combination of your matched-pair antibodies and a corresponding antigen by Improved Enzyme Cycling Method (TN-cyclon™ technology). The kit allows you to detect and quantify your targeted low-abundant protein without large and expensive dedicated equipment.

* High sensitivity measurement cannot always be guaranteed depending on the combination of antibodies and antigens, antibody reactivity, epitope relationship, etc.
*PD-L1 TN-cyclon^TM ELISA Kit is also available. This kit is validated to quantify human PD-L1 in serum or exosome-containing samples. For more details, click here!
* This product is for research use only.

Short movie of TN-cyclon™ (improved enzyme cycling method)

What is improved enzyme cycling method (TN-cyclon™)?
Features of improved enzyme cycling method (TN-cyclon™)
Assay example
Kit components
Product information
Related product

What is improved enzyme cycling method (TN-cyclon™)?

The improved enzyme cycling method (TN-cyclon™) is a unique protein detection technology developed by BioPhenoMA that combines sandwich ELISA and enzyme cycling methods. This technology enables more sensitive detection than conventional sandwich ELISAs.

Principle

In this method, the ELISA method is used as the first reaction to sandwich an antigen by capture and detection antibodies. The detection antibody is labeled with Alkaline Phosphatase (ALP) and works with A3P which is a unique BioPhenoMA’s ALP substrate. Conventional ELISAs quantify the amount of antigen by measurement of the reaction product (shown as A3 in the figure below). In contrast, the initial reaction product is used as the next substrate of the cycling reaction in the improved enzyme cycling method (TN-cyclon™). In the cycling reaction, A3’ is generated by dehydrogenation (oxidation) of A3P and A3 that is the reaction product with ALP by 3α-hydroxysteroid-dehydrogenase (3α-HSD). In addition, the reverse reaction also occurs repeatedly. As the cycling reaction proceeds, the coenzymes thio-NAD and NADH are consumed and the by-products from the consumption, thio-NADH and NAD, are accumulated. Maximum absorbance of this thio-NADH is 405 nm and measurement of the wavelength change of the maximum absorbance enable to estimate the original concentration of the antigen.
The amplification method allows to measure the amount of an ultra-low-abundant antigen that is lower than detection limit by sensitivity of the conventional ELISA.

Reference

1. Watabe, S., et al., Biophysics, 10, 49-54 (2014). [PMID：27493498]
2. Kobayashi, Y., et al., Front. Immunol., 15, 1445771 (2024). [PMID：39687610]

Go back to index

Features of improved enzyme cycling method (TN-cyclon™)

Ultra-sensitive protein detection.
Simple method and no dedicated equipment required^*1.
Applicable to a wide variety of biomarkers^*2.

*1 Absorbance plate reader is required for measurement.
*2 Please prepare your own matched-pair antibodies (capture and detection antibodies) to your targeted antigen.

Go back to index

Assay example

The experimental results were obtained according to the assay protocol described in the user manual. The data were provided by BioPhenoMA.
* Obtained results and values by the kit may not necessarily be the same as the examples below because the measurement results may differ depending on the selected antibodies and antigens.

Standard curve

The left graph shows the calibration curve of the standard PD-L1 concentrations (pg/ml) obtained from the absorbance data (405 nm (Δ0min)). The right table shows the absorbance and the coefficient of variation (CV) data of each PD-L1 concentration.
Measurement wavelength : 405 nm (Primary Wavelength), 660 nm (Secondary Wavelength), Measurement time: 60 min

Limit of detection

The limit of detection, calculated using the standard deviation (SD) of the blank absorbance (3SD applied) and the slope of the calibration curve, was below 0.2 pg/mL (0.154 pg/mL in the example above).

Spike and recovery test (PD-L1 in serum)

PD-L1 with a known concentration (100pg/ml) was added to serum samples and the recovery rate of it was calculated.
* The serum samples were diluted 100 fold before use in Sample Diluent.

Go back to index

Kit components

Reagents

Capture Ab (Antibody) Diluent
Wash Buffer
Sample Diluent
Blocking Reagent
Detection Ab Diluent
Enzyme Cycling Diluents (2 types)
Enzyme Cycling Reagents (4 types)

Accessories

96-well plate (1 plate)
Adhesive Plate Seals (5 units)
User Manual

* Please refer to the user manual (the product datasheet) for other required materials not supplied with the kit. The user manual can be downloaded in from Product information part below.

Go back to index

Product information

[Date : February 25 2026 00:09]

Detail Product Name Product Code Supplier Size Price

TN-cyclon ELISA Development Kit
DatasheetThis may not be the latest data sheet. BPMA-TNEDVA-1e BPM 1 kit $2,002

Contact

Description	You can construct a detection system for low-abundance proteins by this TN-cyclon technology using the antibodies and antigens you have. It does not require special equipment and can be measured with an ordinary absorption plate reader. *High sensitivity measurement cannot always be guaranteed depending on the combination of antibodies and antigens, antibody reactivity, epitope relationship, etc.
Storage	4°C,-20°C	CAS
Link	PD-L1 TN-cyclon™ ELISA Kit

[Date : February 25 2026 00:09]

TN-cyclon ELISA Development Kit

DatasheetThis may not be the latest data sheet.

Product Code: BPMA-TNEDVA-1e
Supplier: BPM
Size: 1kit
Price: $2,002

Description	You can construct a detection system for low-abundance proteins by this TN-cyclon technology using the antibodies and antigens you have. It does not require special equipment and can be measured with an ordinary absorption plate reader. *High sensitivity measurement cannot always be guaranteed depending on the combination of antibodies and antigens, antibody reactivity, epitope relationship, etc.
Storage	4°C,-20°C	CAS
Link	PD-L1 TN-cyclon™ ELISA Kit

Contact

Go back to index

Related product

PD-L1 measurement kit by improved enzyme cycling method (TN-cyclon™)

[Date : February 25 2026 00:09]

Detail Product Name Product Code Supplier Size Price

PD-L1 TN-cyclon ELISA Kit
DatasheetThis may not be the latest data sheet. BPMA-TNPDL1-1e BPM 1 kit $2,968

Contact

Description	The measurement of PD-L1 with this kit is based on TN-cyclon, a proprietary technology that combines the sandwich ELISA and enzymatic cycling methods. This method enables highly sensitive quantification of human PD-L1 in serum or exosome samples. No special equipment is required, and the assay can be performed with an ordinary absorption plate reader. Assay samples: Serum, Exosomes. Cross-species: Human. Detection limit: 0.2 pg/ml or less.
Storage	4°C,-20°C,-80°C	CAS
Link	PD-L1 TN-cyclon™ ELISA Kit

[Date : February 25 2026 00:09]

PD-L1 TN-cyclon ELISA Kit

DatasheetThis may not be the latest data sheet.

Product Code: BPMA-TNPDL1-1e
Supplier: BPM
Size: 1kit
Price: $2,968

Description	The measurement of PD-L1 with this kit is based on TN-cyclon, a proprietary technology that combines the sandwich ELISA and enzymatic cycling methods. This method enables highly sensitive quantification of human PD-L1 in serum or exosome samples. No special equipment is required, and the assay can be performed with an ordinary absorption plate reader. Assay samples: Serum, Exosomes. Cross-species: Human. Detection limit: 0.2 pg/ml or less.
Storage	4°C,-20°C,-80°C	CAS
Link	PD-L1 TN-cyclon™ ELISA Kit

Contact

Go back to index

CONTACT

export@funakoshi.co.jp

※Prices on our website are for your reference only. Please inquire your distributor for your prices.
※Please note that Product Information or Price may change without notice.