HOME> 試薬> 抗体> チラコイド膜関連タンパク質抗体

チラコイド膜関連タンパク質抗体

掲載日情報:2017/12/01 現在Webページ番号:65095

Thylakoid membrane Antibody

チラコイド膜関連タンパク質抗体の検索

下記プルダウンから「因子名」を選択して下さい。検索をクリックすると、別タブで価格表がご覧いただけます。

目次に戻る

使用例

抗AtpF抗体(CF0I subunit of ATP synthase、#AS10-1604)

20 µg of chloroplast fraction from (1) Arabidopsis thaliana (シロイヌナズナ)and (2) Spinacia oleracea(ホウレンソウ)were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 2 % non-fat milk powder in 1xTBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5,000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10,000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions.

抗CP43抗体(IsiA homolog of plant CP43、#AS06-111)

5 µg of total protein from (1) Synechocystis sp. PCC 6803(シネコシスティス) extracted with PEB (#AS08-300), (2) Synechocystis sp. PCC 6803 grown in low iron culture, extracted with PEB, were separated on 4~12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1,000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:20,000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

抗PsbA抗体(D1 protein of PSII, C-terminal、#AS05-084)

2 µg of total protein from (1) Arabidopsis thaliana leaf(シロイヌナズナ葉) extracted with Protein Extration Buffer, PEB (#AS08-300), (2) Hordeum vulgare leaf (オオムギ葉)extracted with PEB, (3) Chlamydomonas reinhardtii (クラミドモナス)total cell extracted with PEB, (4) Synechococcus sp. 7942(シネココッカス7942株) total cell extracted with PEB, (5) Anabaena sp.(アナベナ) total cell extracted with PEB were separated on 4~12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50,000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody, #AS09-602) diluted to 1:50,000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).


目次に戻る

関連製品:抽出用バッファー

植物組織や藻類の細胞を効率良く溶解し、総タンパク質を可溶化するための抽出用バッファーです。
詳細はこちらをご覧下さい。

[在庫・価格 :2024年03月29日 10時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

[在庫・価格 :2024年03月29日 10時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Protein Extraction Buffer, 4× <PEB>

文献数: 8



目次に戻る

関連製品:ウエスタンブロット測定用のキット

光合成タンパク質関連抗体、スタンダード、二次抗体をセットにしたウエスタンブロット測定用のキットです。
詳細は「Photosynthesis Tool Kit / Educational Antibody Kit」をご覧下さい。

[在庫・価格 :2024年03月29日 10時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Photosynthesis Tool Kit, quantitation, for Anti-PsaC, Anti-PsbA, Anti-RbcL Antibodies
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
法規制等
保存条件 法規備考
掲載カタログ

製品記事 Photosynthesis Tool Kit / Educational Antibody Kit
関連記事
Educational antibody Kit, photosynthesis
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
法規制等
保存条件 法規備考
掲載カタログ

製品記事 Photosynthesis Tool Kit / Educational Antibody Kit
関連記事

[在庫・価格 :2024年03月29日 10時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Photosynthesis Tool Kit, quantitation, for Anti-PsaC, Anti-PsbA, Anti-RbcL Antibodies

文献数: 0

説明文 Genbank No: 844754
Protein Accession No: O03042 P05698 P0C510 P00877 P62090 P69416 P0C360 Q00914 Q31QV2 P83755 P0C434 Q14FH6 Q6YXN7 P07753 P14660
法規制等
保存条件 法規備考
掲載カタログ

製品記事 Photosynthesis Tool Kit / Educational Antibody Kit
関連記事

Educational antibody Kit, photosynthesis

文献数: 0

説明文
法規制等
保存条件 法規備考
掲載カタログ

製品記事 Photosynthesis Tool Kit / Educational Antibody Kit
関連記事

目次に戻る

お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。