Anti-mCherry, Rabbit-Poly | GeneTex International Corporation

GTX128508 IP Image
mcherry antibody immunoprecipitates mcherry protein in IP experiments.
IP Sample: 293T whole cell lysate/extract
A : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cells
B : Control with 2.5 ug of pre-immune rabbit IgG
C : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody (GTX128508) 7.5% SDS-PAGE
The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128508) diluted at 1 : 3000.
EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Image
mCherry antibody detects mCherry (full length) protein by western blot analysis.
A. 5ng recombinant mCherry
B. 10ng recombinant mCherry
C. 20ng recombinant mCherry
12% SDS-PAGE
mCherry antibody (GTX128508) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ICC/IF Image
mCherry antibody detects mCherry protein at mCherry fusion protien by immunofluorescent analysis.
Sample: mCherry fusion protien-transfected HeLa cells were fixed in Triton X-100 for 1 min, then 4% paraformaldehyde at RT for 30 min.
Green: mCherry protein stained by mCherry antibody (GTX128508) diluted at 1:500.
Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.

IHC-Wm Image
mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
mCherry antibody labels mCherry-expressing cells (GTX128508) dilution: 1:100.

WB Image
mCherry antibody detects mCherry protein by Western blot analysis. Non-transfected (-) and PLK4 -transfected (+, inculding mCherry -tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:5000.