GTX128508_41297_WB.jpg
GTX128508_41297_IFA.jpg
GTX128508_41297_20141017_IHC-Wm_Z.jpg
GTX128508_42109_20150806_WB_B.jpg
7.5% SDS-PAGE
The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128508) diluted at 1 : 3000.
EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent. )
 protein by western blot analysis.<br />A. 5ng recombinant mCherry<br />B. 10ng recombinant mCherry<br />C. 20ng recombinant mCherry<br />12% SDS-PAGE<br />mCherry antibody (GTX128508) dilution: 1:2000 <br />The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.)
 diluted at 1:500.<br> Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.<br>)
 dilution: 1:100.<BR/>)
 and PLK4 -transfected (+, inculding mCherry -tag) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:5000.)