抗AKT (phospho Ser473)抗体 | Anti-AKT (phospho Ser473) antibody
掲載日情報:2020/04/13 現在Webページ番号:45578
GeneTex社の抗AKT (phospho Ser473)抗体 | Anti-AKT (phospho Ser473) antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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⇒抗AKT (phospho Ser473)抗体(#GTX128414)のユーザーレビューはこちら。
Rating | ![]() ![]() ![]() ![]() ![]() | ( Average 4 based on 1 users reviews) |
Application | ||
Western Blot(WB) | ![]() ![]() ![]() ![]() ![]() | ( Average 4 based on 1 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年04月29日 20時55分現在]
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Anti-AKT (pSer473/474/472), Phosphorylated, Rabbit |
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Anti-AKT (pSer473/474/472), Phosphorylated, Rabbit |
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[在庫・価格 :2025年04月29日 20時55分現在]
Anti-AKT (pSer473/474/472), Phosphorylated, Rabbit
文献数: 62
- 商品コード:GTX128414
- メーカー:GNT
- 包装:100μl
- 価格:¥85,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:AKT serine/threonine kinase 1,AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA Genbank No: 207 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Mouse/Pig/Rat | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | GeneTex社 前立腺がん研究用抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-AKT (pSer473/474/472), Phosphorylated, Rabbit
文献数: 62
- 商品コード:GTX128414
- メーカー:GNT
- 包装:25μl
- 価格:¥30,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:AKT serine/threonine kinase 1,AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA Genbank No: 207 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Mouse/Pig/Rat | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | GeneTex社 前立腺がん研究用抗体 使いっきり抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
![]() | Immunoprecipitation of Akt1/2/3 (phospho Ser473/474/472) protein from 293T whole cell extracts using 5 μg of Akt1/2/3 (phospho Ser473/474/472) antibody (GTX128414). Western blot analysis was performed using Akt1/2/3 (phospho Ser473/474/472) antibody (GTX128414). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. |
![]() | Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. |
![]() | ![]() Untreated (–) and treated (+) HUVEC whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() MCF-7 cells were untreated or treated with 500 nM PMA for 1, 2, 4 and 6 hrs. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | The WB analysis of AKT (phospho Ser473) antibody was published by Sun W and colleagues in the journal Cell Death Dis in 2014 . PMID: 24457964 |
![]() | ![]() Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. |
![]() | AKT (phospho Ser473) antibody detects AKT (phospho Ser473) protein by immunofluorescent analysis.Sample: NIH3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AKT (phospho Ser473) stained by AKT (phospho Ser473) antibody (GTX128414) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm. |
![]() | ![]() The WB analysis of AKT (phospho Ser473) antibody was published by Vallejo-Flores G and colleagues in the journal Biomed Res Int in 2015.PMID: 26557697 |
![]() | ![]() The WB analysis of AKT (phospho Ser473) antibody was published by Lin JG and colleagues in the journal Int J Mol Sci in 2017.PMID: 28837077 |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IP |
Reactivity | Human, Mouse, Rat |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | Assay dependent |
IP | 1:100-1:500 |
Calculated MW | 56 kDa. ( Note ) |
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Positive Control | PC-12 , Rat2 , MCF-7 , MCF-7(500 nM PMA treatment for 1 hr) , MCF-7(500 nM PMA treatment for 2 hr) , MCF-7(500 nM PMA treatment for 4 hr) , MCF-7(500 nM PMA treatment for 6 hr) , HUVEC , HUVEC (5 ng/ml VEGF treatment for 10 min) , NIH-3T3 , NIH-3T3 (starvation for 16 hr and 5 ng/ml PDGF treatment for 15 min) |
Specificity/Sensitivity | This antibody is raised against human AKT1 phosphorylated at Ser473. Based on sequence homology, it is predicted to react with AKT2 and AKT3 when phosphorylated at the corresponding residues - AKT2 Ser474 and AKT3 Ser472. |
Predict Reactivity | Zebrafish, Sheep, Cat, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.06 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human AKT (phospho Ser473/474/472). The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA |
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Cellular Localization | Cytoplasm , Nucleus , Cell membrane |
Background | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] |
Database | ・ Gene ID: 207 AKT1 ・ UniProt: P31749 AKT1 |
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REFERENCE
Application Reference | Application/Reactivity |
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Li H et al. Neurotox Res 2020; 37 (4) : 996-1008 Aluminum-Induced Synaptic Plasticity Impairment via PI3K-Akt-mTOR Signaling Pathway. | Application : WB Reactivity : Rat |
Wang P et al. J Exp Clin Cancer Res 2020; 39 (1) : 7 lncTUG1/miR-144-3p affect the radiosensitivity of esophageal squamous cell carcinoma by competitively regulating c-MET. | Application : WB Reactivity : Human |
Lin MW et al. Antioxidants (Basel) 2019; 9 (1) Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy. | Application : WB Reactivity : Human |
Yan L et al. Lab Invest 2019; Inhibition of microRNA-21-5p reduces keloid fibroblast autophagy and migration by targeting PTEN after electron beam irradiation. | Application : WB Reactivity : Human |
Li X et al. ACS Nano 2019; Gadofullerene Nanoparticles Reverse Dysfunctions of Pancreas and Improve Hepatic Insulin Resistance for Type 2 Diabetes Mellitus Treatment. | Application : WB Reactivity : Human |
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