抗LC3B抗体 | Anti-LC3B antibody
掲載日情報：2020/04/13 現在Webページ番号：45387
GeneTex社の抗LC3B抗体 | Anti-LC3B antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
⇒抗LC3B抗体(#GTX127375)のユーザーレビューはこちら。
| Rating |   | ( Average 4.3 based on 11 users reviews) |
| Application | ||
| Western Blot(WB) | | ( Average 4.3 based on 11 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 ：2024年04月17日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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[在庫・価格 ：2024年04月17日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-LC3B, Rabbit-Poly
文献数： 86
- 商品コード：GTX127375
- メーカー：GNT
- 包装：25μl
- 価格：¥24,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
レビューあり。 別名：microtubule associated protein 1 light chain 3 beta，ATG8F，LC3B，MAP1A/1BLC3，MAP1LC3B-a Genbank No： 81631 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Rabbit | ||||
| 交差性 | Guinea Pig/Human/Mosquito/Mouse/Pig/Rat | 適用 | Electron Microscopy,FCM,IC,IF,IHC,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
使いっきり抗体 |
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| 関連記事 |
GeneTex社における抗体の品質管理 |
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Anti-LC3B, Rabbit-Poly
文献数： 86
- 商品コード：GTX127375
- メーカー：GNT
- 包装：100μl
- 価格：¥75,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
レビューあり。 別名：microtubule associated protein 1 light chain 3 beta，ATG8F，LC3B，MAP1A/1BLC3，MAP1LC3B-a Genbank No： 81631 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Rabbit | ||||
| 交差性 | Guinea Pig/Human/Mosquito/Mouse/Pig/Rat | 適用 | Electron Microscopy,FCM,IC,IF,IHC,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
GeneTex社 オルガネラマーカー抗体 アポトーシス・オートファジー・ネクローシス関連抗体 |
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| 関連記事 |
GeneTex社における抗体の品質管理 |
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DATA IMAGES
 | LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining. |
 |  Wild-type (WT) and LC3B knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. |
 |  LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 20 μg Huh7 whole cell lysate/extract (untreated) B. 20 μg Huh7 whole cell lysate/extract (3uM-Thapsigargin treatment for 12hr) LC3B antibody (GTX127375) dilution: 1:1500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 |  Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with LC3B antibody (GTX127375) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody ) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. |
 | LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LC3B stained by LC3B antibody (GTX127375) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm. |
 | LC3B antibody (GTX127375) detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B antibody (GTX127375) dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter. |
 | LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 50 μg Rat brain lysate/extract 15% SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min. Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm. |
 | HepG2 cells were untreated or treated with 3 μM thapsigargin for 16 and 24 hrs. Whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | LC3B antibody detects LC3B protein in HCV-infected samples by western blot analysis. A. 20 μg Huh7 whole cell lysate/extract (un-infected) B. 20 μg Huh7 whole cell lysate/extract (HCV-infected) LC3B antibody (GTX127375) dilution: 1:1500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | Immunoprecipitation of LC3B protein from U87-MG whole cell extracts using 5 μg of LC3B antibody (GTX127375). Western blot analysis was performed using LC3B antibody (GTX127375). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. |
 | LC3B antibody detects MAP1LC3B protein by western blot analysis. A. 50 μg mouse brain lysate/extract 15% SDS-PAGE LC3B antibody (GTX127375) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | LC3B antibody detects LC3B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.LC3B stained by LC3B antibody (GTX127375) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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製品情報
| Host | Rabbit |
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| Clonality | Polyclonal |
| Isotype | IgG |
| Application | WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP |
| Reactivity | Human, Mouse, Rat, Pig |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.| Application | Dilution |
|---|---|
| WB | 1:500-1:3000 |
| ICC/IF | 1:100-1:1000 |
| IHC-P | 1:100-1:1000 |
| IHC-Fr | Assay dependent |
| FACS | 1:50-1:200 |
| IP | 1:100-1:500 |
| Calculated MW | 15 kDa. ( Note ) |
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| Positive Control | NT2D1 , PC-3 , U87-MG , SK-N-SH , mouse brain , Rat brain , HepG2 (untreated) , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 16hr) , HepG2 (3 μM Thapsigargin treatment for 24hr) , Huh7 (un-infected) , Huh7 (HCV-infected) , HeLa , HeLa (50μM Chloroquine treatment for 24 hr) |
| Specificity/Sensitivity | Cross-reactivity may occur with other LC3 isoforms |
| Predict Reactivity | Zebrafish, Bovine, Japanese Medaka, Chimpanzee, Rhesus Monkey(>80% identity) |
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PROPERTIES
| Form | Liquid |
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| Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Concentration | 0.14 mg/ml (Please refer to the vial label for the specific concentration.) |
| Antigen Species | Human |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
| Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
| Synonyms | microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a |
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| Cellular Localization | Cytoplasm , Endomembrane system; Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane; Lipid-anchor |
| Background | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq] |
| Database | ・ Gene ID: 81631 MAP1LC3B ・ UniProt: Q9GZQ8 MAP1LC3B |
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REFERENCE
| Application Reference | Application/Reactivity |
|---|---|
| Viana GM et al. J Clin Med 2020; 9 (2) Brain Pathology in Mucopolysaccharidoses (MPS) Patients with Neurological Forms. | Application : IHC-Fr Reactivity : Human |
| Dapas B et al. Int J Pharm 2020; 574 : 118895 Effects of eEF1A1 targeting by aptamer/siRNA in chronic lymphocytic leukaemia cells. | Application : WB Reactivity : Human |
| Ho KH et al. Neurotherapeutics 2020; (Epub) A Key Role of DNA Damage-Inducible Transcript 4 (DDIT4) Connects Autophagy and GLUT3-Mediated Stemness To Desensitize Temozolomide Efficacy in Glioblastomas. | Application : WB Reactivity : Human |
| Xia M et al. Int Immunopharmacol 2020; 80 : 106125 Dihydroartemisinin inhibits the proliferation of IgAN mesangial cells through the mTOR signaling pathway. | Application : WB Reactivity : Human |
| Lin MW et al. Antioxidants (Basel) 2019; 9 (1) Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy. | Application : WB Reactivity : Human |
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