抗HIF1 alpha抗体 | Anti-HIF1 alpha antibody

掲載日情報:2020/04/13 現在Webページ番号:45353

GeneTex社の抗HIF1 alpha抗体 | Anti-HIF1 alpha antibodyです。

本製品は研究用です。研究用以外には使用できません。

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抗HIF1 alpha抗体(#GTX127309)のユーザーレビューはこちら。

Ratingrating star full rating star full rating star full rating star full rating star half( Average 4.2 based on 16 users reviews)
Application
Western Blot(WB)rating star full rating star full rating star full rating star full rating star half( Average 4.4 based on 11 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)rating star full rating star full rating star full rating star full rating star empty( Average 4 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)rating star full rating star full rating star full rating star half rating star empty( Average 3.7 based on 3 users reviews)
IHC (Frozen sections)(IHC-Fr)rating star full rating star full rating star full rating star full rating star empty( Average 4 based on 1 users reviews)

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年04月19日 18時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-HIF-1α, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 154
説明文
レビューあり。KO/KDバリデーション済み抗体。抗原:aa 657~826
別名:hypoxia inducible factor 1 subunit alpha,HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
Genbank No: 3091
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Bovine/Human/Mouse/Rabbit/Rat 適用 ChIP,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 がん転移関連因子抗体
抗HIF-1α抗体
低酸素症関連抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理
Anti-HIF-1α, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 154
説明文
レビューあり。KO/KDバリデーション済み抗体。抗原:aa 657~826
別名:hypoxia inducible factor 1 subunit alpha,HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
Genbank No: 3091
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Bovine/Human/Mouse/Rabbit/Rat 適用 ChIP,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ ニュース2018年11月15日号 p.61
100周年ありがとうキャンペーン p.54

製品記事 がん転移関連因子抗体
抗HIF-1α抗体
低酸素症関連抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年04月19日 18時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-HIF-1α, Rabbit-Poly

文献数: 154

説明文 レビューあり。KO/KDバリデーション済み抗体。抗原:aa 657~826
別名:hypoxia inducible factor 1 subunit alpha,HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
Genbank No: 3091
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Bovine/Human/Mouse/Rabbit/Rat 適用 ChIP,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 がん転移関連因子抗体
抗HIF-1α抗体
低酸素症関連抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

Anti-HIF-1α, Rabbit-Poly

文献数: 154

説明文 レビューあり。KO/KDバリデーション済み抗体。抗原:aa 657~826
別名:hypoxia inducible factor 1 subunit alpha,HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
Genbank No: 3091
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Bovine/Human/Mouse/Rabbit/Rat 適用 ChIP,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ ニュース2018年11月15日号 p.61
100周年ありがとうキャンペーン p.54

製品記事 がん転移関連因子抗体
抗HIF-1α抗体
低酸素症関連抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES

HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis.
Samples: Untreated (left panel) and 500μM CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min
Green: HIF1alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:1000
Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody (GTX11304) diluted at 1:2500
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Images captured by Olympus FV10i Confocal Laser Scanning Microscope

HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney cancer.
HIF1 alpha antibody (GTX127309) dilution: 1:500.
Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309).
Western blot analysis was performed using HIF1 alpha antibody (GTX127309) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:200.
Blue: Hoechst 33342 staining.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B.30 μg HepG2 whole cell lysate/extract ( 200 μM CoCl2 treatment for 24 hr)
C. 30 μg HepG2 whole cell lysate/extract (500 μM CoCl2 treatment for 24 hr)
7.5% SDS-PAGE
HIF1 alpha antibody (GTX127309) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The WB analysis of HIF1 alpha antibody was published by Offer S and colleagues in the journal J Exp Clin Cancer Res in 2019 . PMID: 31174567
The WB analysis of HIF1 alpha antibody was published by Kudová J and colleagues in the journal PLoS One in 2016 . PMID: 27355368

The WB analysis of HIF1 alpha antibody was published by Demetriades C and colleagues in the journal Nat Commun in 2016.PMID: 26868506

HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF1 alpha stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay
ReactivityHuman, Mouse, Rat, Rabbit, Bovine


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:570
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW 93 kDa. ( Note )
Positive Control MCF-7 , HeLa(500uM CoCl for 24hr) , NIH-3T3 (500 μM CoCl2 treatment for 24 hr) , HepG2 whole cell lysate/extract (1% O2 treatment for 24hr) , 293T transfected HIF1A , HepG2 ( 200 μM CoCl2 treatment for 24 hr) , HepG2 (500 μM CoCl2 treatment for 24 hr)
Specificity/Sensitivity Knockdown/Knockout validation was supported by references (PMID:25428606)
Predict Reactivity Dog, Pig(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.26 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78
Cellular Localization Cytoplasm , Nucleus
Background Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Database ・ Gene ID: 3091 HIF1A
・ UniProt: Q16665 HIF1A


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REFERENCE

Application ReferenceApplication/Reactivity
Kato H et al. Oncogene. 2020; (Epub) Cancer-derived UTX TPR mutations G137V and D336G impair interaction with MLL3/4 complexes and affect UTX subcellular localization.Application : WB
Reactivity : Human
Fujii M et al. Int Endod J 2020; (Epub) Hypoxia-inducible factor 1α promotes interleukin 1β and tumour necrosis factor α expression in lipopolysaccharide-stimulated human dental pulp cells.Application : WB
Reactivity : Human
Zhang S et al. Gene 2020; 733 : 144383 MicroRNA-574-5p in gastric cancer cells promotes angiogenesis by targeting protein tyrosine phosphatase non-receptor type 3 (PTPN3).Application : WB
Reactivity : Human
Chen W et al. Oncol Lett 2019; 17 (3) : 2867-2873 Clinical implications of hypoxia-inducible factor-1α and caveolin-1 overexpression in isocitrate dehydrogenase-wild type glioblastoma multiforme.
Chua KV et al. Cells 2019; 9 (1) Octyl Gallate Induces Pancreatic Ductal Adenocarcinoma Cell Apoptosis and Suppresses Endothelial-Mesenchymal Transition-Promoted M2-Macrophages, HSP90帢 Secretion, and Tumor Growth.Application : WB
Reactivity : Human


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