抗HIF1 alpha抗体 | Anti-HIF1 alpha antibody
掲載日情報:2020/04/13 現在Webページ番号:45353
GeneTex社の抗HIF1 alpha抗体 | Anti-HIF1 alpha antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
GeneTex社 ホームページでユーザーレビューが閲覧できます
GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
⇒抗HIF1 alpha抗体(#GTX127309)のユーザーレビューはこちら。
Rating | ![]() ![]() ![]() ![]() ![]() | ( Average 4.2 based on 16 users reviews) |
Application | ||
Western Blot(WB) | ![]() ![]() ![]() ![]() ![]() | ( Average 4.4 based on 11 users reviews) |
Immunocytochemistry/ Immunofluorescence(ICC/IF) | ![]() ![]() ![]() ![]() ![]() | ( Average 4 based on 1 users reviews) |
IHC (Paraffin sections)(IHC-P) | ![]() ![]() ![]() ![]() ![]() | ( Average 3.7 based on 3 users reviews) |
IHC (Frozen sections)(IHC-Fr) | ![]() ![]() ![]() ![]() ![]() | ( Average 4 based on 1 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年04月26日 14時15分現在]
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Anti-HIF-1α, Rabbit-Poly |
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[在庫・価格 :2025年04月26日 14時15分現在]
Anti-HIF-1α, Rabbit-Poly
文献数: 177
- 商品コード:GTX127309
- メーカー:GNT
- 包装:25μl
- 価格:¥30,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。KO/KDバリデーション済み抗体。抗原:aa 657~826 別名:hypoxia inducible factor 1 subunit alpha,HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78 Genbank No: 3091 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Bovine/Human/Mouse/Rabbit/Rat | 適用 | ChIP,IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | がん転移関連因子抗体 抗HIF-1α抗体 低酸素症関連抗体 使いっきり抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-HIF-1α, Rabbit-Poly
文献数: 177
- 商品コード:GTX127309
- メーカー:GNT
- 包装:100μl
- 価格:¥85,000
- 在庫:2個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。KO/KDバリデーション済み抗体。抗原:aa 657~826 別名:hypoxia inducible factor 1 subunit alpha,HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78 Genbank No: 3091 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Bovine/Human/Mouse/Rabbit/Rat | 適用 | ChIP,IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
100周年ありがとうキャンペーン p.54 ニュース2018年11月15日号 p.61
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製品記事 | がん転移関連因子抗体 抗HIF-1α抗体 低酸素症関連抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
![]() | HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis. Samples: Untreated (left panel) and 500μM CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min Green: HIF1alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:1000 Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody (GTX11304) diluted at 1:2500 Blue: Hoechst 33342 staining. Scale bar = 10 μm. Images captured by Olympus FV10i Confocal Laser Scanning Microscope |
![]() | ![]() HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() ![]() Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis. Sample: Paraffin-embedded human kidney cancer. HIF1 alpha antibody (GTX127309) dilution: 1:500. |
![]() | Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309). Western blot analysis was performed using HIF1 alpha antibody (GTX127309) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. |
![]() | ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter. |
![]() | HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:200. Blue: Hoechst 33342 staining. |
![]() | ![]() Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. A. 30 μg HepG2 whole cell lysate/extract (untreated) B.30 μg HepG2 whole cell lysate/extract ( 200 μM CoCl2 treatment for 24 hr) C. 30 μg HepG2 whole cell lysate/extract (500 μM CoCl2 treatment for 24 hr) 7.5% SDS-PAGE HIF1 alpha antibody (GTX127309) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | The WB analysis of HIF1 alpha antibody was published by Offer S and colleagues in the journal J Exp Clin Cancer Res in 2019 . PMID: 31174567 |
![]() | The WB analysis of HIF1 alpha antibody was published by Kudová J and colleagues in the journal PLoS One in 2016 . PMID: 27355368 |
![]() | ![]() The WB analysis of HIF1 alpha antibody was published by Demetriades C and colleagues in the journal Nat Commun in 2016.PMID: 26868506 |
![]() | ![]() HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF1 alpha stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm. |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay |
Reactivity | Human, Mouse, Rat, Rabbit, Bovine |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Fr | Assay dependent |
IP | 1:100-1:570 |
ChIP assay | Assay dependent |
Calculated MW | 93 kDa. ( Note ) |
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Positive Control | MCF-7 , HeLa(500uM CoCl for 24hr) , NIH-3T3 (500 μM CoCl2 treatment for 24 hr) , HepG2 whole cell lysate/extract (1% O2 treatment for 24hr) , 293T transfected HIF1A , HepG2 ( 200 μM CoCl2 treatment for 24 hr) , HepG2 (500 μM CoCl2 treatment for 24 hr) |
Specificity/Sensitivity | Knockdown/Knockout validation was supported by references (PMID:25428606) |
Predict Reactivity | Dog, Pig(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.26 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78 |
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Cellular Localization | Cytoplasm , Nucleus |
Background | Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq] |
Database | ・ Gene ID: 3091 HIF1A ・ UniProt: Q16665 HIF1A |
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REFERENCE
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