抗AKT [N3C2], Internal抗体 | Anti-AKT [N3C2], Internal antibody

掲載日情報:2020/04/13 現在Webページ番号:44543

GeneTex社の抗AKT [N3C2], Internal抗体 | Anti-AKT [N3C2], Internal antibodyです。

本製品は研究用です。研究用以外には使用できません。

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抗AKT [N3C2], Internal抗体(#GTX121937)のユーザーレビューはこちら。

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Application
Western Blot(WB)rating star full rating star full rating star full rating star full rating star half( Average 4.2 based on 5 users reviews)

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年05月18日 10時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-AKT, Rabbit
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 97
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:AKT antibody,MGC99656 antibody,PKB antibody,PKB-ALPHA antibody,PRKBA antibody,RAC antibody,RAC-ALPHA antibody,AKT1 antibody,protein kinase B alpha antibody,rac protein kinase alpha antibody,RAC-alpha serine/threonine-protein kinase antibody
Genbank No: 207
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Japanese Medaka/Mouse/Pig/Rat 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事
関連記事 GeneTex社における抗体の品質管理
Anti-AKT1+2+3, Rabbit
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 97
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:AKT antibody,MGC99656 antibody,PKB antibody,PKB-ALPHA antibody,PRKBA antibody,RAC antibody,RAC-ALPHA antibody,AKT1 antibody,protein kinase B alpha antibody,rac protein kinase alpha antibody,RAC-alpha serine/threonine-protein kinase antibody
Genbank No: 207
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Japanese Medaka/Mouse/Pig/Rat 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月18日 10時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-AKT, Rabbit

文献数: 97

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:AKT antibody,MGC99656 antibody,PKB antibody,PKB-ALPHA antibody,PRKBA antibody,RAC antibody,RAC-ALPHA antibody,AKT1 antibody,protein kinase B alpha antibody,rac protein kinase alpha antibody,RAC-alpha serine/threonine-protein kinase antibody
Genbank No: 207
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Japanese Medaka/Mouse/Pig/Rat 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事 GeneTex社における抗体の品質管理

Anti-AKT1+2+3, Rabbit

文献数: 97

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:AKT antibody,MGC99656 antibody,PKB antibody,PKB-ALPHA antibody,PRKBA antibody,RAC antibody,RAC-ALPHA antibody,AKT1 antibody,protein kinase B alpha antibody,rac protein kinase alpha antibody,RAC-alpha serine/threonine-protein kinase antibody
Genbank No: 207
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Japanese Medaka/Mouse/Pig/Rat 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES

Sample (30 μg of whole cell lysate)
A: JC
B: BCL-1
7.5% SDS PAGE
GTX121937 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 μg of Akt1/2/3 antibody [N3C2], Internal (GTX121937).
Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal (GTX121937).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Sample (30 μg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX121937 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Staining of Human normal esophagus tissue sections using anti-Akt1/2/3 antibody (GTX121937).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
AKT antibody [N3C2], Internal detects AKT protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
The WB analysis of AKT antibody [N3C2], Internal was published by Sun W and colleagues in the journal Cell Death Dis in 2014 . PMID: 24457964
The WB analysis of AKT antibody [N3C2], Internal was published by Vallejo-Flores G and colleagues in the journal Biomed Res Int in 2015.PMID: 26557697


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IHC-Fr, IP, IHC
ReactivityHuman, Mouse, Rat, Japanese Medaka


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW 56 kDa. ( Note )
Positive Control 293T , A431 , HeLa , HepG2 , Neuro2A , NIH-3T3 , Raw264.7 , JC , BCL-1, PC-12 , C2C12 , HA-human Akt -transfected HeLa , *mouse melanoma
Specificity/Sensitivity IP/MS validation was supported by references (PMID:30377414)
Predict Reactivity Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human Akt1/2/3. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, AKT1 antibody, protein kinase B alpha antibody, rac protein kinase alpha antibody, RAC-alpha serine/threonine-protein kinase antibody, proto-oncogene c-Akt antibody, RAC-PK-alpha antibody, PKB alpha antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, AKT2 antibody, protein kinase B beta antibody, murine thymoma viral (v-akt) homolog-2 antibody, protein kinase Akt-2 antibody, RAC-PK-beta antibody, PKB beta antibody, rac protein kinase beta antibody, RAC-beta serine/threonine-protein kinase antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, DKFZp434N0250 antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AKT3 antibody, PKB gamma antibody, RAC-gamma serine/threonine-protein kinase antibody, RAC-gamma serine/threonine protein kinase antibody, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
Cellular Localization Cytoplasm , Nucleus , Cell membrane
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]


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REFERENCE

Application ReferenceApplication/Reactivity
Li H et al. Neurotox Res 2020; 37 (4) : 996-1008 Aluminum-Induced Synaptic Plasticity Impairment via PI3K-Akt-mTOR Signaling Pathway.Application : WB
Reactivity : Rat
Wang J et al. Am J Physiol Cell Physiol 2020; (Epub) The Physiological Modulation of Intracellular kinases on Hippocampal Gamma Oscillation in Vitro.Application : WB
Reactivity : Rat
Chen SY et al. Stem Cells Transl Med 2020; (Epub) Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.Application : WB
Reactivity : Human
Lu CC et al. Int J Oncol 2019; 54 (4) : 1271-1281 Metformin triggers the intrinsic apoptotic response in human AGS gastric adenocarcinoma cells by activating AMPK and suppressing mTOR/AKT signaling.
Chen H et al. Theranostics 2019; 9 (26) : 8321-8331 Pten loss in Lgr5+ hair follicle stem cells promotes SCC development.Application : WB
Reactivity : Mouse


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