抗AKT [N3C2], Internal抗体 | Anti-AKT [N3C2], Internal antibody
掲載日情報:2020/04/13 現在Webページ番号:44543
GeneTex社の抗AKT [N3C2], Internal抗体 | Anti-AKT [N3C2], Internal antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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⇒抗AKT [N3C2], Internal抗体(#GTX121937)のユーザーレビューはこちら。
Rating | ( Average 4.2 based on 5 users reviews) | |
Application | ||
Western Blot(WB) | ( Average 4.2 based on 5 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2024年05月18日 10時35分現在]
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Anti-AKT, Rabbit |
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Anti-AKT1+2+3, Rabbit |
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[在庫・価格 :2024年05月18日 10時35分現在]
Anti-AKT, Rabbit
文献数: 97
- 商品コード:GTX121937
- メーカー:GNT
- 包装:100μl
- 価格:¥75,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
レビューあり。KO/KDバリデーション済み抗体。 別名:AKT antibody,MGC99656 antibody,PKB antibody,PKB-ALPHA antibody,PRKBA antibody,RAC antibody,RAC-ALPHA antibody,AKT1 antibody,protein kinase B alpha antibody,rac protein kinase alpha antibody,RAC-alpha serine/threonine-protein kinase antibody Genbank No: 207 |
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別包装品 | 別包装品あり | ||||||
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保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Japanese Medaka/Mouse/Pig/Rat | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | |||||||
関連記事 |
GeneTex社における抗体の品質管理 |
Anti-AKT1+2+3, Rabbit
文献数: 97
- 商品コード:GTX121937
- メーカー:GNT
- 包装:25μl
- 価格:¥24,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
レビューあり。KO/KDバリデーション済み抗体。 別名:AKT antibody,MGC99656 antibody,PKB antibody,PKB-ALPHA antibody,PRKBA antibody,RAC antibody,RAC-ALPHA antibody,AKT1 antibody,protein kinase B alpha antibody,rac protein kinase alpha antibody,RAC-alpha serine/threonine-protein kinase antibody Genbank No: 207 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Japanese Medaka/Mouse/Pig/Rat | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
使いっきり抗体 |
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関連記事 |
GeneTex社における抗体の品質管理 |
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DATA IMAGES
Sample (30 μg of whole cell lysate) A: JC B: BCL-1 7.5% SDS PAGE GTX121937 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 μg of Akt1/2/3 antibody [N3C2], Internal (GTX121937). Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal (GTX121937). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. | |
Sample (30 μg of whole cell lysate) A: PC-12 7.5% SDS PAGE GTX121937 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Staining of Human normal esophagus tissue sections using anti-Akt1/2/3 antibody (GTX121937). | |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. | |
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. | |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
AKT antibody [N3C2], Internal detects AKT protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm. | |
The WB analysis of AKT antibody [N3C2], Internal was published by Sun W and colleagues in the journal Cell Death Dis in 2014 . PMID: 24457964 | |
The WB analysis of AKT antibody [N3C2], Internal was published by Vallejo-Flores G and colleagues in the journal Biomed Res Int in 2015.PMID: 26557697 |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC |
Reactivity | Human, Mouse, Rat, Japanese Medaka |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | Assay dependent |
IHC-Fr | Assay dependent |
IP | 1:100-1:500 |
IHC | Assay dependent |
Calculated MW | 56 kDa. ( Note ) |
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Positive Control | 293T , A431 , HeLa , HepG2 , Neuro2A , NIH-3T3 , Raw264.7 , JC , BCL-1, PC-12 , C2C12 , HA-human Akt -transfected HeLa , *mouse melanoma |
Specificity/Sensitivity | IP/MS validation was supported by references (PMID:30377414) |
Predict Reactivity | Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.3 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Recombinant protein encompassing a sequence within the center region of human Akt1/2/3. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, AKT1 antibody, protein kinase B alpha antibody, rac protein kinase alpha antibody, RAC-alpha serine/threonine-protein kinase antibody, proto-oncogene c-Akt antibody, RAC-PK-alpha antibody, PKB alpha antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, AKT2 antibody, protein kinase B beta antibody, murine thymoma viral (v-akt) homolog-2 antibody, protein kinase Akt-2 antibody, RAC-PK-beta antibody, PKB beta antibody, rac protein kinase beta antibody, RAC-beta serine/threonine-protein kinase antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, DKFZp434N0250 antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AKT3 antibody, PKB gamma antibody, RAC-gamma serine/threonine-protein kinase antibody, RAC-gamma serine/threonine protein kinase antibody, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody |
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Cellular Localization | Cytoplasm , Nucleus , Cell membrane |
Background | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011] |
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REFERENCE
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