抗Glutamine synthetase抗体 | Anti-Glutamine synthetase antibody

掲載日情報:2020/04/02 現在Webページ番号:39093

GeneTex社の抗Glutamine synthetase抗体 | Anti-Glutamine synthetase antibodyです。

本製品は研究用です。研究用以外には使用できません。

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抗Glutamine synthetase抗体(#GTX109121)のユーザーレビューはこちら。

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Application
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特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年05月18日 09時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-GLUL, Rabbit-Poly <Anti-Glutamine Synthetase>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 13
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:glutamate-ammonia ligase,GLNS,GS,PIG43,PIG59
Genbank No: 2752
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理
Anti-GLUL, Rabbit-Poly <Anti-Glutamine Synthetase>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 13
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:glutamate-ammonia ligase,GLNS,GS,PIG43,PIG59
Genbank No: 2752
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月18日 09時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-GLUL, Rabbit-Poly <Anti-Glutamine Synthetase>

文献数: 13

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:glutamate-ammonia ligase,GLNS,GS,PIG43,PIG59
Genbank No: 2752
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

Anti-GLUL, Rabbit-Poly <Anti-Glutamine Synthetase>

文献数: 13

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:glutamate-ammonia ligase,GLNS,GS,PIG43,PIG59
Genbank No: 2752
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES


Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Glutamine synthetase antibody detects Glutamine synthetase protein at astrocytes by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.Sample:Paraffin-embedded adult mouse retina. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
Immunohistochemical analysis of paraffin-embedded H441 xenograft , using Glutamine Synthetase (GTX109121) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation of Glutamine synthetase protein from IMR32 whole cell extracts using 5 μg of Glutamine synthetase antibody (GTX109121).Western blot analysis was performed using Glutamine synthetase antibody (GTX109121).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Glutamine synthetase antibody detects Glutamine synthetase protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody (GTX109121) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Glutamine synthetase antibody detects Glutamine synthetase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:20000.
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX109121) diluted at 1:50000.


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IHC-Fr, IP, IHC
ReactivityHuman, Mouse, Rat


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW 42 kDa. ( Note )
Positive Control 293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , mouse eye , rat brain
Predict Reactivity Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Full length human Glutamine synthetase Recombinant protein.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59
Cellular Localization Cytoplasm
Background Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]
Database ・ Gene ID: 2752 GLUL
・ UniProt: P15104 GLUL


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REFERENCEE(抜粋)

Application ReferenceApplication/Reactivity
Mundorf A et al. Psychiatry Res 2019; 281 : 112544 Lithium and glutamine synthetase: Protective effects following stress.Application : IHC
Reactivity : Mouse
Pronin A et al. Front Mol Neurosci 2019; 12 : 36 Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury.
Nakashima K et al. Sci Rep 2019; 9 (1) : 14818 A spatial similarity of stereochemical environments formed by amino acid residues defines a common epitope of two non-homologous proteins.Application : ICC/IF
Reactivity : Human
Kamei Y et al. Am J Physiol Endocrinol Metab 2014; 307 (6) : E485-93 FOXO1 activates glutamine synthetase gene in mouse skeletal muscles through a region downstream of 3'-UTR: possible contribution to ammonia detoxification.


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reagent@funakoshi.co.jp

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