抗SOX2 [N1C3]抗体 | Anti-SOX2 [N1C3] antibody
掲載日情報：2020/04/13 現在Webページ番号：35626
GeneTex社の抗SOX2 [N1C3]抗体 | Anti-SOX2 [N1C3] antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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⇒抗SOX2 [N1C3]抗体(#GTX101507)のユーザーレビューはこちら。
| Rating |   | ( Average 3 based on 1 users reviews) |
| Application | ||
| Western Blot(WB) | | ( Average 3 based on 1 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 ：2024年04月26日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-SOX2, Rabbit-Poly <Anti-SRY-Box 2> |
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Anti-Sox2, Rabbit-Poly |
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57 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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[在庫・価格 ：2024年04月26日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-SOX2, Rabbit-Poly <Anti-SRY-Box 2>
文献数： 57
- 商品コード：GTX101507
- メーカー：GNT
- 包装：25μl
- 価格：¥24,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
レビューあり。KO/KDバリデーション済み抗体。抗原：aa 1～251 別名：SRY-box 2，ANOP3，MCOPS3 Genbank No： 6657 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Rabbit | ||||
| 交差性 | Ceratotherium simum/Frog/Human/Mouse/Rat | 適用 | FCM,IC,IF,IHC,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
中枢神経系（CNS）マーカー抗体 使いっきり抗体 |
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| 関連記事 |
GeneTex社における抗体の品質管理 |
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Anti-Sox2, Rabbit-Poly
文献数： 57
- 商品コード：GTX101507
- メーカー：GNT
- 包装：100μl
- 価格：¥75,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
レビューあり。KO/KDバリデーション済み抗体。抗原：aa 1～251 別名：SRY-box 2，ANOP3，MCOPS3 Genbank No： 6657 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Rabbit | ||||
| 交差性 | Ceratotherium simum/Frog/Human/Mouse/Rat | 適用 | FCM,IC,IF,IHC,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
ニュース2022年6月15日号 p.34
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| 製品記事 |
中枢神経系（CNS）マーカー抗体 |
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| 関連記事 |
GeneTex社における抗体の品質管理 |
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DATA IMAGES
 | SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 |  Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. |
 | Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507). Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. |
 | SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500. |
 | Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GTX101507) antibody at 1:80 dilution. |
 | SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. SOX2 antibody [N1C3] (GTX101507) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
 | SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fetal brain. Green: SOX2 antibody [N1C3] (GTX101507) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
 | SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507) or SOX2 antibody [GT1876] (GTX627404). Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. |
 |  Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920). |
 | SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920). |
 | Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GTX101507) antibody at 1:160 dilution. |
 | Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101507)antibody at 1:100 dilution(purple) or rabbit IgG (black). |
 | Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI (GTX30920). |
 | Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GTX101507) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
 | SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 μm. |
 | The ICC/IF analysis of SOX2 antibody [N1C3] was published by Chang WF and colleagues in the journal PLoS One in 2016.PMID: 27802323 |
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製品情報
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Application | WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP |
| Reactivity | Human, Mouse, Rat |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.| Application | Dilution |
|---|---|
| WB | 1:5000-1:20000 |
| ICC/IF | 1:100-1:1000 |
| IHC-P | 1:100-1:1000 |
| IHC-Fr | 1:100-1:1000 |
| FACS | 1:50-1:200 |
| IP | 1:100-1:500 |
| Calculated MW | 34 kDa. ( Note ) |
|---|---|
| Positive Control | NT2D1 , hESC , Mouse ESC |
| Predict Reactivity | Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity) |
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PROPERTIES
| Form | Liquid |
|---|---|
| Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Concentration | 0.15 mg/ml (Please refer to the vial label for the specific concentration.) |
| Antigen Species | Human |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
| Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
| Synonyms | SRY-box 2 , ANOP3 , MCOPS3 |
|---|---|
| Cellular Localization | Nucleus |
| Background | This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq] |
| Database | ・ Gene ID: 6657 SOX2 ・ UniProt: P48431 SOX2 |
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REFERENCE
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製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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