抗beta Catenin [N1N2-2], N-term抗体 | Anti-beta Catenin [N1N2-2], N-term antibody

掲載日情報:2020/04/02 現在Webページ番号:35579

GeneTex社の抗beta Catenin [N1N2-2], N-term抗体 | Anti-beta Catenin [N1N2-2], N-term antibodyです。

本製品は研究用です。研究用以外には使用できません。

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抗beta Catenin [N1N2-2], N-term抗体(#GTX101435)のユーザーレビューはこちら。

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Application
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特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年04月27日 15時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-CTNNB1, Rabbit-Poly <Anti-β-Catenin><Anti-Catenin (Cadherin-Associated Protein), β1, 88kDa>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 41
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:catenin beta 1,CTNNB,EVR7,MRD19,armadillo
Genbank No: 1499
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Cat/Dog/Human/Mouse/Rabbit/Rat/Zebrafish 適用 ChIP,FCM,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 Wntシグナル経路関連抗体
使いっきり抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理
Anti-CTNNB1, Rabbit-Poly <Anti-β-Catenin><Anti-Catenin (Cadherin-Associated Protein), β1, 88kDa>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 41
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:catenin beta 1,CTNNB,EVR7,MRD19,armadillo
Genbank No: 1499
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Cat/Dog/Human/Mouse/Rabbit/Rat/Zebrafish 適用 ChIP,FCM,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 Wntシグナル経路関連抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年04月27日 15時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-CTNNB1, Rabbit-Poly <Anti-β-Catenin><Anti-Catenin (Cadherin-Associated Protein), β1, 88kDa>

文献数: 41

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:catenin beta 1,CTNNB,EVR7,MRD19,armadillo
Genbank No: 1499
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Cat/Dog/Human/Mouse/Rabbit/Rat/Zebrafish 適用 ChIP,FCM,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 Wntシグナル経路関連抗体
使いっきり抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理

Anti-CTNNB1, Rabbit-Poly <Anti-β-Catenin><Anti-Catenin (Cadherin-Associated Protein), β1, 88kDa>

文献数: 41

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:catenin beta 1,CTNNB,EVR7,MRD19,armadillo
Genbank No: 1499
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Cat/Dog/Human/Mouse/Rabbit/Rat/Zebrafish 適用 ChIP,FCM,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 Wntシグナル経路関連抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES

beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by western blot analysis.A. 30 μg PC-12 whole cell lysate/extract | 7.5% SDS-PAGEbeta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.Blue: Hoechst 33342 staining.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody (GTX101435).Western blot analysis was performed using beta Catenin antibody (GTX101435).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
beta Catenin antibody [N1N2-2], N-term (GTX101435) detects CTNNB1 protein by flow cytometry analysis. Sample: HeLa cell. Black: Unlabelled sample was used as a control. Red: beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.Blue: Hoechst 33342 staining.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sample (50 μg of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX101435 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin(GTX101435) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human esophagus by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:10000.


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, FACS, IP, ChIP assay
ReactivityHuman, Mouse, Rat, Rabbit


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:50-1:100
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW 85 kDa. ( Note )
Positive Control HeLa , A549 , H1299 , HCT116 , PC-12 , Mouse brain , *human fibroblast , *LX2
Predict Reactivity Zebrafish, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo
Cellular Localization Cytoplasm , Nucleus , cytoskeleton
Background Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]
Database ・ Gene ID: 1499 CTNNB1
・ UniProt: P35222 CTNNB1


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REFERENCEE(抜粋)

Application ReferenceApplication/Reactivity
Jiang X et al. J Stomatol Oral Maxillofac Surg 2020; (Epub) Bone regeneration and mineralisation was promoted during distraction osteogenesis by human periostin gene in rabbit mandibular model.Application : WB
Reactivity : Rabbit
Tsai CH et al. Biochim Biophys Acta Mol Cell Res 2019; 1867 (2) : 118564 Aza-PBHA, a potent histone deacetylase inhibitor, inhibits human gastric-cancer cell migration via PKC帢-mediated AHR-HDAC interactions.Application : WB
Reactivity : Human
Callejas BE et al. Int J Cancer 2019; (Epub) Helminth-derived molecules inhibit colitis-associated colon cancer development through NF-庥B and STAT3 regulation.Application : IHC-P
Reactivity : Mouse
Shen HH et al. J Biomed Sci 2019; 26 (1) : 62 Raloxifene inhibits adipose tissue inflammation and adipogenesis through Wnt regulation in ovariectomized rats and 3?3-L1 cells.Application : WB
Reactivity : Rat
Fang Y et al. Nat Commun 2019; 10 (1) : 3981 CD36 inhibits 帣-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.Application : IHC-P
Reactivity : Human


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(テクニカルサポート 試薬担当)

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