抗E-Cadherin抗体 | Anti-E-Cadherin antibody
掲載日情報:2020/04/13 現在Webページ番号:35107
GeneTex社の抗E-Cadherin抗体 | Anti-E-Cadherin antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
⇒抗E-Cadherin抗体(#GTX100443)のユーザーレビューはこちら。
Rating | ![]() ![]() ![]() ![]() ![]() | ( Average 4.5 based on 2 users reviews) |
Application | ||
Western Blot(WB) | ![]() ![]() ![]() ![]() ![]() | ( Average 4.5 based on 2 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年04月26日 12時55分現在]
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[在庫・価格 :2025年04月26日 12時55分現在]
Anti-E-Cadherin, Rabbit-Poly
文献数: 111
- 商品コード:GTX100443
- メーカー:GNT
- 包装:100μl
- 価格:¥85,000
- 在庫:2個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。KO/KDバリデーション済み抗体。抗原:aa 462~732 別名:cadherin 1,Arc-1,BCDS1,CD324,CDHE,ECAD,LCAM,UVO Genbank No: 999 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Dog/Human/Mouse/Rat/Zebrafish | 適用 | IC,IF,IHC,IP,PLA,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 抗SSEA-5抗体 ゼブラフィッシュ(zebrafish)研究用製品特集 GeneTex社:抗E-カドヘリン抗体 獣医学研究用抗体 VetSignalシリーズ |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-E-Cadherin, Rabbit-Poly
文献数: 111
- 商品コード:GTX100443
- メーカー:GNT
- 包装:25μl
- 価格:¥30,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。KO/KDバリデーション済み抗体。抗原:aa 462~732 別名:cadherin 1,Arc-1,BCDS1,CD324,CDHE,ECAD,LCAM,UVO Genbank No: 999 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Dog/Human/Mouse/Rat/Zebrafish | 適用 | IC,IF,IHC,IP,PLA,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | ゼブラフィッシュ(zebrafish)研究用製品特集 使いっきり抗体 GeneTex社:抗E-カドヘリン抗体 獣医学研究用抗体 VetSignalシリーズ |
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関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
![]() | ![]() Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() ![]() Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody (GTX100443) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. |
![]() | E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. E-cadherin antibody (GTX100443) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
![]() | E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm. |
![]() | E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Blue: Hoechst 33342 staining. |
![]() | E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 3 μg of preimmune Rabbit IgG B. Immunoprecipitation of E-cadherin protein by 3 μg E-cadherin antibody (GTX100443) 5 % SDS-PAGE The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody (GTX100443) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent] |
![]() | Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody (GTX100443). Western blot analysis was performed using E-Cadherin antibody (GTX100443). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. |
![]() | E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443). |
![]() | E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. E-Cadherin antibody (GTX100443) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500. Blue: Hoechst 33342 staining. |
![]() | E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. E-Cadherin antibody (GTX100443) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | The WB analysis of E-Cadherin antibody was published by Tsai CH and colleagues in the journal Sci Rep in 2017.PMID: 28195146 |
![]() | The WB analysis of E-Cadherin antibody was published by Lee AF and colleagues in the journal PLoS One in 2017.PMID: 28683123 |
![]() | The WB analysis of E-Cadherin antibody was published by Lee AF and colleagues in the journal PLoS One in 2017.PMID: 28683123 |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Wm, IP |
Reactivity | Human, Mouse, Rat, Zebrafish |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:10000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Wm | Assay dependent |
IP | 1:100-1:500 |
Calculated MW | 97 kDa. ( Note ) |
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Positive Control | MCF-7 , *HT-29 |
Predict Reactivity | Bovine, Dog, Pig(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.49 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO |
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Cellular Localization | Cell junction , Cell membrane |
Background | This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq] |
Database | ・ Gene ID: 999 CDH1 ・ UniProt: P12830 CDH1 |
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REFERENCE
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