抗E-Cadherin抗体 | Anti-E-Cadherin antibody

掲載日情報:2020/04/13 現在Webページ番号:35107

GeneTex社の抗E-Cadherin抗体 | Anti-E-Cadherin antibodyです。

本製品は研究用です。研究用以外には使用できません。

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抗E-Cadherin抗体(#GTX100443)のユーザーレビューはこちら。

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特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年04月26日 08時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-E-Cadherin, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 94
説明文
レビューあり。KO/KDバリデーション済み抗体。抗原:aa 462~732
別名:cadherin 1,Arc-1,BCDS1,CD324,CDHE,ECAD,LCAM,UVO
Genbank No: 999
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Dog/Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,IP,PLA,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 抗SSEA-5抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
GeneTex社:抗E-カドヘリン抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理
Anti-E-Cadherin, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 94
説明文
レビューあり。KO/KDバリデーション済み抗体。抗原:aa 462~732
別名:cadherin 1,Arc-1,BCDS1,CD324,CDHE,ECAD,LCAM,UVO
Genbank No: 999
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Dog/Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,IP,PLA,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
GeneTex社:抗E-カドヘリン抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年04月26日 08時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-E-Cadherin, Rabbit-Poly

文献数: 94

説明文 レビューあり。KO/KDバリデーション済み抗体。抗原:aa 462~732
別名:cadherin 1,Arc-1,BCDS1,CD324,CDHE,ECAD,LCAM,UVO
Genbank No: 999
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Dog/Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,IP,PLA,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 抗SSEA-5抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
GeneTex社:抗E-カドヘリン抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理

Anti-E-Cadherin, Rabbit-Poly

文献数: 94

説明文 レビューあり。KO/KDバリデーション済み抗体。抗原:aa 462~732
別名:cadherin 1,Arc-1,BCDS1,CD324,CDHE,ECAD,LCAM,UVO
Genbank No: 999
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Dog/Human/Mouse/Rat/Zebrafish 適用 IC,IF,IHC,IP,PLA,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
GeneTex社:抗E-カドヘリン抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES


Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody (GTX100443) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
E-cadherin antibody (GTX100443) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.
E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Rabbit IgG
B. Immunoprecipitation of E-cadherin protein by 3 μg E-cadherin antibody (GTX100443)
5 % SDS-PAGE
The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody (GTX100443) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody (GTX100443).
Western blot analysis was performed using E-Cadherin antibody (GTX100443).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443).
E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
E-Cadherin antibody (GTX100443) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The WB analysis of E-Cadherin antibody was published by Tsai CH and colleagues in the journal Sci Rep in 2017.PMID: 28195146
The WB analysis of E-Cadherin antibody was published by Lee AF and colleagues in the journal PLoS One in 2017.PMID: 28683123
The WB analysis of E-Cadherin antibody was published by Lee AF and colleagues in the journal PLoS One in 2017.PMID: 28683123


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IHC-Wm, IP
ReactivityHuman, Mouse, Rat, Zebrafish


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW 97 kDa. ( Note )
Positive Control MCF-7 , *HT-29
Predict Reactivity Bovine, Dog, Pig(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.49 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO
Cellular Localization Cell junction , Cell membrane
Background This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]
Database ・ Gene ID: 999 CDH1
・ UniProt: P12830 CDH1


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REFERENCE

Application ReferenceApplication/Reactivity
Palazzolo S et al. Cancers (Basel) 2019; 11 (12) An Effective Multi-Stage Liposomal DNA Origami Nanosystem for In Vivo Cancer Therapy.Application : IHC-P
Reactivity : Mouse
Terasaki M et al. J Clin Med 2019; 9 (1) Dietary Fucoxanthin Induces Anoikis in Colorectal Adenocarcinoma by Suppressing Integrin Signaling in a Murine Colorectal Cancer Model.Application : WB
Reactivity : Mouse
Kim H et al. Micromachines (Basel) 2019; 10 (11) Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro.Application : ICC/IF
Reactivity : Mouse
Zhang L et al. Cells 2019; 8 (10) Estimating Dynamic Cellular Morphological Properties via the Combination of the RTCA System and a Hough-Transform-Based Algorithm.Application : WB
Reactivity : Human
Zhang X et al. Evid Based Complement Alternat Med 2019; 2019 : 5370847 Qingshen Buyang Formula Attenuates Renal Fibrosis in 5/6 Nephrectomized Rats via Inhibiting EMT and Wnt/帣-Catenin Pathway.


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