抗GAPDH抗体 | Anti-GAPDH antibody
掲載日情報：2020/04/13 現在Webページ番号：34920
GeneTex社の抗GAPDH抗体 | Anti-GAPDH antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
GeneTex社 ホームページでユーザーレビューが閲覧できます
GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
⇒抗GAPDH抗体(#GTX100118)のユーザーレビューはこちら。
| Rating |   | ( Average 4.6 based on 16 users reviews) |
| Application | ||
| Western Blot(WB) | | ( Average 4.5 based on 15 users reviews) |
| Immunocytochemistry/ Immunofluorescence(ICC/IF) | | ( Average 5 based on 1 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 ：2025年05月06日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-GAPDH, Rabbit-Poly |
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Anti-GAPDH, Rabbit-Poly |
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[在庫・価格 ：2025年05月06日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-GAPDH, Rabbit-Poly
文献数： 1065
- 商品コード：GTX100118
- メーカー：GNT
- 包装：100μl
- 価格：¥85,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | レビューあり。抗原：aa 9～323 別名：glyceraldehyde-3-phosphate dehydrogenase，G3PD，GAPD，HEL-S-162eP Genbank No： 2597 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Rabbit | ||||
| 交差性 | Bacteria/Bovine/Caenorhabditis elegans/Chicken/Dog/Drosophila/E. coli/Fish/Hamster/Human/Insect/Milkfish/Monkey/Mosquito/Mouse/Nematode/Pig/Pika/Plant/Rabbit/Rat/Zebrafish | 適用 | IC,IF,IHC,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
ニュース2021年12月1日号 p.10
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| 製品記事 | GeneTex社 オルガネラマーカー抗体 ローディングコントロール抗体 ゼブラフィッシュ(zebrafish)研究用製品特集 検証済みの組換えウサギモノクローナル抗体 HLクローン |
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| 関連記事 | GeneTex社における抗体の品質管理 |
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Anti-GAPDH, Rabbit-Poly
文献数： 1065
- 商品コード：GTX100118
- メーカー：GNT
- 包装：25μl
- 価格：¥30,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | レビューあり。抗原：aa 9～323 別名：glyceraldehyde-3-phosphate dehydrogenase，G3PD，GAPD，HEL-S-162eP Genbank No： 2597 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Rabbit | ||||
| 交差性 | Bacteria/Bovine/Caenorhabditis elegans/Chicken/Dog/Drosophila/E. coli/Fish/Hamster/Human/Insect/Milkfish/Monkey/Mosquito/Mouse/Nematode/Pig/Pika/Plant/Rabbit/Rat/Zebrafish | 適用 | IC,IF,IHC,IP,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | ローディングコントロール抗体 ゼブラフィッシュ(zebrafish)研究用製品特集 使いっきり抗体 検証済みの組換えウサギモノクローナル抗体 HLクローン |
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| 関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
 | Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody (GTX100118). Western blot analysis was performed using GAPDH antibody (GTX100118). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent. |
 | Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000. |
 | GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 10% SDS-PAGE GAPDH antibody (GTX100118) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | Immunohistochemical analysis of human salivary gland cancer, using GAPDH(GTX100118) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
 | The WB analysis of GAPDH antibody was published by Chen YW and colleagues in the journal Evid Based Complement Alternat Med in 2013 . PMID: 23573126 |
 | Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | The WB analysis of GAPDH antibody was published by Li HY and colleagues in the journal Breast Cancer Res in 2017 . PMID: 29258605 |
 | The WB analysis of GAPDH antibody was published by Yeo AJ and colleagues in the journal Cell Discov in 2015.PMID: 27462424 |
 | The WB analysis of GAPDH antibody was published by Kuo TM and colleagues in the journal J Biomed Sci in 2014.PMID: 25200553 |
 | The WB analysis of GAPDH antibody was published by Chang YC and colleagues in the journal J Cell Mol Med in 2014.PMID: 24400868 |
 | The WB analysis of GAPDH antibody was published by Kuo TM and colleagues in the journal J Biomed Sci in 2014.PMID: 25200553 |
 | The WB analysis of GAPDH antibody was published by Chen JY and colleagues in the journal J Biomed Sci in 2013.PMID: 24359494 |
 | The WB analysis of GAPDH antibody was published by Chang YC and colleagues in the journal J Cell Mol Med in 2014.PMID: 24400868 |
 | The WB analysis of GAPDH antibody was published by Våtsveen TK and colleagues in the journal J Hematol Oncol in 2018.PMID: 29458389 |
 | The WB analysis of GAPDH antibody was published by Lin MY and colleagues in the journal Evid Based Complement Alternat Med in 2013.PMID: 24282433 |
 | The WB analysis of GAPDH antibody was published by Huang YH and colleagues in the journal PLoS One in 2015.PMID: 26305546 |
 | The WB analysis of GAPDH antibody was published by Iervolino A and colleagues in the journal PLoS One in 2015.PMID: 25799508 |
 | The WB analysis of GAPDH antibody was published by Kuo TM and colleagues in the journal J Biomed Sci in 2014.PMID: 25200553 |
 | The WB analysis of GAPDH antibody was published by Chang YC and colleagues in the journal J Cell Mol Med in 2014.PMID: 24400868 |
 | The WB analysis of GAPDH antibody was published by Chang YC and colleagues in the journal J Cell Mol Med in 2014.PMID: 24400868 |
 | The WB analysis of GAPDH antibody was published by Donti TR and colleagues in the journal Dis Model Mech in 2014.PMID: 24271779 |
 | The WB analysis of GAPDH antibody was published by Donti TR and colleagues in the journal Dis Model Mech in 2014.PMID: 24271779 |
 | The WB analysis of GAPDH antibody was published by Yeo AJ and colleagues in the journal Cell Discov in 2015.PMID: 27462424 |
 | The WB analysis of GAPDH antibody was published by Shibaguchi T and colleagues in the journal Physiol Rep in 2016.PMID: 27482075 |
 | The WB analysis of GAPDH antibody was published by Chiu CT and colleagues in the journal PLoS One in 2015.PMID: 25938237 |
 | The WB analysis of GAPDH antibody was published by Chen JY and colleagues in the journal J Biomed Sci in 2013.PMID: 24359494 |
 | The WB analysis of GAPDH antibody was published by Donti TR and colleagues in the journal Dis Model Mech in 2014.PMID: 24271779 |
 | The WB analysis of GAPDH antibody was published by Tung H and colleagues in the journal PLoS One in 2016.PMID: 26863132 |
 | The WB analysis of GAPDH antibody was published by Chiu CT and colleagues in the journal PLoS One in 2015.PMID: 25938237 |
 | GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
 | GAPDH antibody detects GAPDH protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:2000.Blue: Hoechst 33342 staining. |
 | The WB analysis of GAPDH antibody was published by Cheng CW and colleagues in the journal Cell Death Dis in 2013 . PMID: 23788038 |
 | The WB analysis of GAPDH antibody was published by Cheng CW and colleagues in the journal Cell Death Dis in 2013 . PMID: 23788038 |
 | The WB analysis of GAPDH antibody was published by Chen YW and colleagues in the journal Evid Based Complement Alternat Med in 2013 . PMID: 23573126 |
 | GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
 | The WB analysis of GAPDH antibody was published by Chiu CT and colleagues in the journal PLoS One in 2015.PMID: 25938237 |
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製品情報
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Application | WB, ICC/IF, IHC-P, IP |
| Reactivity | Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Drosophila, Bovine, Dog, Hamster, Chicken, Pig, Monkey, E. coli, Mosquito, Nematode, Pika, Fish, Bacteria |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.| Application | Dilution |
|---|---|
| WB | 1:5000-1:100000 |
| ICC/IF | 1:100-1:1000 |
| IHC-P | 1:100-1:1000 |
| IP | 1:100-1:500 |
| Calculated MW | 36 kDa. ( Note ) |
|---|---|
| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , E.coli M15 , *SH-SY5Y , *A549 , *U2OS , *H9C2 , *A7R5 , *mouse skin tissue |
| Predict Reactivity | Sheep, Cat, Guinea pig, Xenopus laevis, Rhesus Monkey(>80% identity) |
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PROPERTIES
| Form | Liquid |
|---|---|
| Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Concentration | 0.29 mg/ml (Please refer to the vial label for the specific concentration.) |
| Antigen Species | Human |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
| Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
| Synonyms | glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP |
|---|---|
| Cellular Localization | Cytoplasm , perinuclear region , Membrane |
| Background | The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq] |
| Database | ・ Gene ID: 2597 GAPDH ・ UniProt: P04406 GAPDH |
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REFERENCE
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製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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