抗G-CSF Receptor抗体(Anti-G-CSF Receptor, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：34314
G-CSF Receptorに対する抗体(Anti-G-CSF Receptor, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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Anti-G-CSF Receptor, Mouse-Mono(38643) <Anti-CD114> |
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Anti-Human G-CSF R/CD114 MAb (Clone 38643) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-G-CSF Receptor, Mouse-Mono(38643) <Anti-CD114>
文献数： 2
- 商品コード：MAB3811
- メーカー：RSD
- 包装：500μg
- 価格：¥121,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human G-CSF R/CD114 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF381，スタンダードとして#381-GR-050を用いる。 別名：CD114 クローン：38643 Genbank No： 1441 Protein Accession No： Q99062 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Mouse | ||||
| 交差性 | Human | 適用 | ELISA,FCM,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human G-CSF R/CD114 MAb (Clone 38643)
文献数： 0
- 商品コード：MAB3811-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：CD114 クローン：38643 Genbank No： 1441 Protein Accession No： Q99062 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human G-CSF R/CD114Glu25-Pro621Accession # Q99062 |
| Source | Monoclonal Mouse IgG1 Clone # 38643 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human G-CSF R/CD114 in ELISAs and Western blots. In sandwich Immunoassays, no cross-reactivity with recombinant human (rh) G‑CSF, rhGM‑CSF R beta, rhGM‑CSF R, or recombinant mouse G‑CSF is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.5 µg/mL | See below |
| Flow Cytometry | 2.5 µg/106 cells | Human whole blood monocytes and human whole blood granulocytes |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Human G-CSF R/CD114 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human G‑CSF R/CD114 Antibody (Catalog #MAB3811 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human G-CSF R/CD114 Biotinylated Antibody (Catalog #BAF381 ) |
| ELISA Standard | Recombinant Human G-CSF R/CD114 Protein (Catalog #381-GR ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Western Blot |
 click image to view larger | Detection of Human G‑CSF R/CD114 by Western Blot.Western blot shows lysates of AML‑193 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human G‑CSF R/CD114 Monoclonal Antibody (Catalog # MAB3811) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for G‑CSF R/ CD114 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: G-CSF R/CD114 | Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant. Cell activation by G-CSF is mediated by a type I membrane protein belonging to the cytokine receptor superfamily. Human G-CSF R is 863 amino acids (aa) in length, with a 604 aa extracellular domain, a 26 aa transmembrane domain, and a 183 aa cytoplasmic domain that include a 23 amino acid signal sequence. As a result of alternative splicing, at least four isoforms of G-CSF R that differ in their C-terminal region exist. Isoform 2 lacks the transmembrane region and may represent a soluble form of the receptor; however the existence of soluble G-CSF R in human serum has not been reported (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood (2). Human and mouse G-CSF R have a homology of 62.5%. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (1). |
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References
| Nicola, N.A., in Cytokine Reference, (2001) Oppenhiem, J.J. and M. Feldmann, eds. Academic Press p. 1935 | |
| Mitsui, T. et al. (2003) Blood. 101:2990. | |
| Long Name: | Granulocyte Colony Stimulating Factor Receptor |
| Entrez Gene IDs: | 1441 (Human); 12986 (Mouse) |
| Alternate Names: | CD114 antigen; CD114; colony stimulating factor 3 receptor (granulocyte); CSF3R; Csfgr; G-CSF R; G-CSF receptor; GCSFR; G-CSFR; GCSFRG-CSF-R; granulocyte colony-stimulating factor receptor |
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Related Research Areas
| Granulocyte Markers | ||
| IL-6 Family | ||
| Monocyte Markers | ||
| Receptors in the Jak/STAT Pathway | ||
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| Western Blot | |
|---|---|
 | Detection of Human G‑CSF R/CD114 by Western Blot. Western blot shows lysates of AML‑193 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human G‑CSF R/CD114 Monoclonal Antibody (Catalog # MAB3811) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for G‑CSF R/CD114 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Related Product & Information
| Background | G-CSF R/CD114 |
|---|---|
| background_content | Background: G-CSF R/CD114 Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant. Cell activation by G-CSF is mediated by a type I membrane protein belonging to the cytokine receptor superfamily. Human G-CSF R is 863 amino acids (aa) in length, with a 604 aa extracellular domain, a 26 aa transmembrane domain, and a 183 aa cytoplasmic domain that include a 23 amino acid signal sequence. As a result of alternative splicing, at least four isoforms of G-CSF R that differ in their C-terminal region exist. Isoform 2 lacks the transmembrane region and may represent a soluble form of the receptor; however the existence of soluble G-CSF R in human serum has not been reported (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood (2). Human and mouse G-CSF R have a homology of 62.5%. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (1). |
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Citations
- Most purported antibodies to the human granulocyte colony-stimulating factor receptor are not specific.
Authors: deBruin C, Lincoln P, Hartley C, Shehabeldin A, Van G, Szilvassy S
Exp Hematol, 2010;38(11):1022-35.
Species: Human
Sample Type: Cell Lysates
Application: WB
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