抗CD34抗体(Anti-CD34, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant rat CD34Glu37-Ile293Accession # NP_001100672
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rat CD34 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant porcine CD34 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Rat CD34
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry

CD34 in Rat Liver. CD34 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Rat CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4117) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Entrez Gene IDs	947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Background	CD34
background_content	Background: CD34 Rat CD34 is a 80-100 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352 amino acid (aa) protein with a 257 aa extracellular domain (ECD) (aa 35-291) and 74 aa cytoplasmic tail (aa 313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively.

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Citations

1. Cold water swimming pretreatment reduces cognitive deficits in a rat model of traumatic brain injury
   Authors: ZW Zhou, YD Li, WW Gao, JL Chen, SY Yue, JN Zhang
   Neural Regen Res, 2017;12(8):1322-1328.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC-P


2. Rat umbilical cord blood cells attenuate hypoxic-ischemic brain injury in neonatal rats
   Authors: K Nakanishi, Y Sato, Y Mizutani, M Ito, A Hirakawa, Y Higashi
   Sci Rep, 2017;7(0):44111.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


3. Responses of adventitial CD34(+) vascular wall-resident stem/progenitor cells and medial smooth muscle cells to carotid injury in rats
   Authors: Jian Song
   Exp. Mol. Pathol., 2016;0(0):.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


4. Systemic siRNA Nanoparticle-Based Drugs Combined with Radiofrequency Ablation for Cancer Therapy.
   Authors: Ahmed M, Kumar G, Navarro G, Wang Y, Gourevitch S, Moussa M, Rozenblum N, Levchenko T, Galun E, Torchilin V, Goldberg S
   PLoS ONE, 2015;10(7):e0128910.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Not Specified


5. A metastatic hepatoma model of rats using the 13762-MAT-B-III cell line: basic characteristics and potential as a tool for interventional oncology experiments.
   Authors: Choi J, Kim H, Baek S, Ryu Y, Chung J
   Anticancer Res, 2015;35(3):1333-8.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Renal telocytes contribute to the repair of ischemically injured renal tubules.
   Authors: Li L, Lin M, Li L, Wang R, Zhang C, Qi G, Xu M, Rong R, Zhu T
   J Cell Mol Med, 2014;18(6):1144-56.
   Species: Rat
   Sample Type: Whole Cells
   Application: IF


7. Rat hair follicle-constituting cells labeled by a newly-developed somatic stem cell-recognizing antibody: a possible marker of hair follicle development.
   Authors: Ichikawa C, Izawa T, Juniantito V, Tanaka M, Hori M, Tanaka K, Takenaka S, Kuwamura M, Yamate J
   Histol Histopathol, 2013;28(2):257-68.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. In vivo adipogenesis in rats measured by cell kinetics in adipocytes and plastic-adherent stroma-vascular cells in response to high-fat diet and thiazolidinedione.
   Authors: Tchoukalova YD, Fitch M, Rogers PM, Covington JD, Henagan TM, Ye J, Hellerstein MK, Ravussin E
   Diabetes, 2012;61(1):137-44.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC

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