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抗Canine VEGF Biotinylated抗体(Anti-Canine VEGF Biotinylated antibody)

掲載日情報：2021/01/28 現在Webページ番号：33474

アールアンドディーシステムズ /
R&D Systems, Inc.
[メーカー略称：RSD]

Canine VEGF Biotinylatedに対する抗体(Anti-Canine VEGF Biotinylated )です。
※ 本製品は研究用です。研究用以外には使用できません。

価格
Product Details
Applications and Data
Data Examples
References
Related Research Areas
Related Product & Information
Citations

価格

[在庫・価格：2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

詳細

商品名

商品コード
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包装
価格
在庫
法規制等

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文献数

Anti-Canine VEGF Biotinylated Affinity Purified PAb (50 UG)

データシート※最新のデータシートでない場合があります

BAF1603
RSDアールアンドディーシステムズ
R&D Systems, Inc.
50 μg
¥111,000
無 (未発注)

10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。

マイリストに追加マイリスト追加済

説明文	マッチドペア：Canine VEGF サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB1603，スタンダードとして#1603-CV-010を用いる。別名：MVCD1 Genbank No： 7422 Protein Accession No： Q9MYV3.3
法規制等
保存条件				法規備考
抗原種	Canine	免疫動物	Goat	クラス	IgG	標識	Biotin
交差性	Canine			適用	ELISA,IC,Western Blot
クロナリティ	Polyclonal	フォーマット		性状	Antigen Affinity Purified	吸収処理
掲載カタログ
製品記事	M1/M2 Macrophage Activation Marker Myeloid-derived Suppressor Cell Marker
関連記事	R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格：2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Canine VEGF Biotinylated Affinity Purified PAb (50 UG)

文献数： 1

データシート※最新のデータシートでない場合があります

商品コード：BAF1603
メーカー：RSD
包装：50μg
価格：¥111,000
在庫：無(未発注)
納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
法規制等：

説明文	マッチドペア：Canine VEGF サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB1603，スタンダードとして#1603-CV-010を用いる。別名：MVCD1 Genbank No： 7422 Protein Accession No： Q9MYV3.3
法規制等
保存条件		法規備考
抗原種	Canine	免疫動物	Goat
交差性	Canine	適用	ELISA,IC,Western Blot
標識	Biotin	性状	Antigen Affinity Purified
吸収処理		クラス	IgG
クロナリティ	Polyclonal	フォーマット
掲載カタログ
製品記事	M1/M2 Macrophage Activation Marker Myeloid-derived Suppressor Cell Marker
関連記事	R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

マイリストに追加マイリスト追加済

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Product Details

Species Reactivity	Canine
Label	Biotin
Immunogen	E. coli-derived recombinant canine VEGF164Pro28-Arg190Accession # Q9MYV3.3
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects canine VEGF164 in ELISAs and Western blots. In sandwich immunoassays, 100% cross-reactivity with recombinant human VEGF is observed and less than 2% cross-reactivity with recombinant rat VEGF and recombinant mouse VEGF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Canine VEGF (Catalog # 1603-CV)
Immunocytochemistry	5-15 µg/mL	See below
Canine VEGF Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Canine VEGF Antibody (Catalog #MAB1603 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Canine VEGF₁₆₄ Biotinylated Antibody (Catalog #BAF1603 )
ELISA Standard		Recombinant Canine VEGF Protein (Catalog #1603-CV )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunocytochemistry	click image to view larger	VEGF in Canine PBMCs.VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine VEGF 164 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1603) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF	Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternately spliced isoforms of VEGF ranging from 206 amino acids (aa) to 121 aa in length are known. Three isoforms, VEGF₁₈₈, VEGF₁₈₂, and VEGF₁₆₄, have been identified in canine. Canine VEGF₁₆₄ shares 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline homologs, respectively. Two type I transmembrane receptor tyrosine kinases, VEGF R1 and VEGF R2, that bind VEGF with high affinity, have been identified. Neuropilin-1, a receptor for semaphorin, also binds VEGF and acts as a co‑receptor to enhance the affinity between VEGF and VEGF R2. Neuropilin-1 alone can also mediate VEGF-induced endothelial cell migration. VEGF regulates cell proliferation, migration, and survival of endothelial cells. These functions are partially mediated through the induction of nitric oxide, prostacyclin, and metalloproteinases. Together with angiopoietins or other vascular-specific growth factors, VEGF plays a separate but complementary role in angiogenesis and vasculogenesis (1‑7).

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References

JingJing, L. et al. (2000) Mol. Cell Biochem. 214:23.
Scheidegger, P. et al. (1999) Biol. Chem. 380:1449.
Thurston, G. (2002) J. Anat. 200:575.
Zachary, I. and Gliki, G. (2001) Cardiovasc. Res. 49:568.
Chevalier, S. (2002) Mol. Cell Endocrinol. 189:169.
Robinson, C.J. and Stringer, S.E. (2001) J. Cell. Sci. 114:853.
Wang, L. et al. (2003) J. Biol. Chem. 278:48848.
Long Name:	Vascular Endothelial Growth Factor
Entrez Gene IDs:	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names:	MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

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Related Research Areas

Adipocytokines
Adult Neurogenesis
Airway Inflammation and Remodeling
Alpha Granule Soluble Molecules
ALS Related Molecules
Cancer Biomarkers
Developmental Factors Implicated in Neurodegeneration
HIF Transcription Factors
Inflammatory Mediators
Macrophage Activation Markers
Myeloid-derived Suppressor Cells (MDSC)
Preeclampsia
VEGF Family
VEGF Family Ligands
A functional comparison of canine and murine bone marrow derived cultured mast cells. Authors: Lin TY, London CA, Vet. Immunol. Immunopathol., 2006;114(3):320-34. Species: Canine Sample Type: Cell Culture Supernates Application: ELISA Development

Immunocytochemistry
	VEGF in Canine PBMCs. VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine VEGF 164 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1603) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

VEGF in Canine PBMCs.
VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine VEGF 164 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1603) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

目次に戻る

Related Product & Information

Background	VEGF
background_content	Background: VEGF Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternately spliced isoforms of VEGF ranging from 206 amino acids (aa) to 121 aa in length are known. Three isoforms, VEGF188, VEGF182, and VEGF164, have been identified in canine. Canine VEGF164 shares 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline homologs, respectively. Two type I transmembrane receptor tyrosine kinases, VEGF R1 and VEGF R2, that bind VEGF with high affinity, have been identified. Neuropilin-1, a receptor for semaphorin, also binds VEGF and acts as a co‑receptor to enhance the affinity between VEGF and VEGF R2. Neuropilin-1 alone can also mediate VEGF-induced endothelial cell migration. VEGF regulates cell proliferation, migration, and survival of endothelial cells. These functions are partially mediated through the induction of nitric oxide, prostacyclin, and metalloproteinases. Together with angiopoietins or other vascular-specific growth factors, VEGF plays a separate but complementary role in angiogenesis and vasculogenesis (1‑7).

Background

VEGF

background_content

Background:
VEGF
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternately spliced isoforms of VEGF ranging from 206 amino acids (aa) to 121 aa in length are known. Three isoforms, VEGF188, VEGF182, and VEGF164, have been identified in canine. Canine VEGF164 shares 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline homologs, respectively. Two type I transmembrane receptor tyrosine kinases, VEGF R1 and VEGF R2, that bind VEGF with high affinity, have been identified. Neuropilin-1, a receptor for semaphorin, also binds VEGF and acts as a co‑receptor to enhance the affinity between VEGF and VEGF R2. Neuropilin-1 alone can also mediate VEGF-induced endothelial cell migration. VEGF regulates cell proliferation, migration, and survival of endothelial cells. These functions are partially mediated through the induction of nitric oxide, prostacyclin, and metalloproteinases. Together with angiopoietins or other vascular-specific growth factors, VEGF plays a separate but complementary role in angiogenesis and vasculogenesis (1‑7).

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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

A functional comparison of canine and murine bone marrow derived cultured mast cells.
Authors: Lin TY, London CA,
Vet. Immunol. Immunopathol., 2006;114(3):320-34.
Species: Canine
Sample Type: Cell Culture Supernates
Application: ELISA Development

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