抗Human/Mouse Myeloperoxidase抗体(Anti-Human/Mouse Myeloperoxidase antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse MPO Met16-Thr718Accession # P11247
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Immunohistochemistry
	Myeloperoxidase/MPO in Human Spleen. Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot
	Detection of Human/Mouse Myeloperoxidase/MPO by Western Blot. Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Myeloperoxidase/MPO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Simple Western
	Detection of Mouse Myeloperoxidase/MPO by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Immunocytochemistry
	Myeloperoxidase/MPO in Mouse Splenocytes. Myeloperoxidase/MPO was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Entrez Gene IDs	4353 (Human); 17523 (Mouse); 303413 (Rat)
Background	Myeloperoxidase/MPO
background_content	Background: Myeloperoxidase/MPO Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.

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Citations

1. Mesenchymal stem cells attenuate blood-brain barrier leakage after cerebral ischemia in mice
   Authors: Z Cheng, L Wang, M Qu, H Liang, W Li, Y Li, L Deng, Z Zhang, GY Yang
   J Neuroinflammation, 2018;15(1):135.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-F


2. Topical Fibronectin Improves Wound Healing of Irradiated Skin
   Authors: MB Johnson, B Pang, DJ Gardner, S Niknam-Ben, V Soundaraja, A Bramos, DP Perrault, K Banks, GK Lee, RY Baker, GH Kim, S Lee, Y Chai, M Chen, W Li, L Kwong, YK Hong, AK Wong
   Sci Rep, 2017;7(1):3876.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Obesity promotes prolonged ovalbumin-induced airway inflammation modulating Th1, Th2 and Th17 immune responses in BALB/c mice
   Authors: FM Silva, EE Oliveira, AC Gouveia, AS Brugiolo, CC Alves, JO Correa, J Gameiro, J Mattes, HC Teixeira, AP Ferreira
   Clin. Exp. Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Cell-penetrating interactomic inhibition of nuclear factor-kappa B in a mouse model of postoperative cognitive dysfunction
   Authors: SY Cheon, JM Kim, EH Kam, CC Ho, EJ Kim, S Chung, JH Jeong, DD Lee, SW Lee, BN Koo
   Sci Rep, 2017;7(1):13482.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


5. Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation
   Authors: M Toussaint, DJ Jackson, D Swieboda, A Guedán, TD Tsouroukts, YM Ching, C Radermecke, H Makrinioti, J Aniscenko, MR Edwards, R Solari, F Farnir, V Papayannop, F Bureau, T Marichal, SL Johnston
   Nat. Med., 2017;23(6):681-691.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis
   Authors: CWL Dougher, A Buffone, MJ Nemeth, M Nasirikena, EE Irons, PN Bogner, JTY Lau
   J. Leukoc. Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


7. Neutrophil Attack Triggers Extracellular Trap-Dependent Candida Cell Wall Remodeling and Altered Immune Recognition
   PLoS Pathog, 2016;12(5):e1005644.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC Not Specified


8. Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium
   Infect Immun, 2016;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


9. Swimming Motility Mediates the Formation of Neutrophil Extracellular Traps Induced by Flagellated Pseudomonas aeruginosa
   PLoS Pathog., 2016;12(11):e1005987.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IF


10. PAD4 Deficiency Decreases Inflammation and Susceptibility to Pregnancy Loss in a Mouse Model
    Authors: Luise Erpenbeck
    Biol. Reprod, 2016;95(6):132.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-P

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