抗IFN-γ Receptor 1抗体(Anti-IFN-γ Receptor 1, Rat-Mono antibody)
掲載日情報:2021/01/28 現在Webページ番号:32022
IFN-γ Receptor 1に対する抗体(Anti-IFN-γ Receptor 1, Rat-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年04月26日 20時55分現在]
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Anti-IFN-γ Receptor 1, Rat-Mono(170911) |
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Anti-Mouse IFN-gamma R1 MAb (Clone 170911) |
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[在庫・価格 :2025年04月26日 20時55分現在]
Anti-IFN-γ Receptor 1, Rat-Mono(170911)
文献数: 1
- 商品コード:MAB10262
- メーカー:RSD
- 包装:500μg
- 価格:¥122,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Mouse IFN-γ R1 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF1026,スタンダードとして#1026-GR-100を用いる。 別名:AVP クローン:170911 Genbank No: 3459 Protein Accession No: NP_034641 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Mouse | 免疫動物 | Rat | ||||
交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse IFN-gamma R1 MAb (Clone 170911)
文献数: 0
- 商品コード:MAB10262-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:AVP クローン:170911 Genbank No: 3459 Protein Accession No: NP_034641 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse IFN-gamma R1/CD119Gly23-Asp253 (predicted)Accession # NP_034641 |
Source | Monoclonal Rat IgG2B Clone # 170911 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects mouse IFN-gamma R1/CD119 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) IFN‑ alpha / beta R1, rmIFN-gamma R2, recombinant human (rh) IFN-gamma R1, rhIL-20 R alpha, rhIL-20 R beta, rmIL-20 R alpha, rhIL-22BP or rmIL-22BP is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Mouse IFN-gamma R1 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse IFN‑ gamma R1/CD119 Antibody (Catalog #MAB10262 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse IFN‑ gamma R1/CD119 Biotinylated Antibody (Catalog #BAF1026 ) |
ELISA Standard | Recombinant Mouse IFN-gamma R1 Fc Chimera Protein, CF (Catalog #1026-GR ) | |
Neutralization | Measured by its ability to neutralize IFN‑ gamma R1/CD119-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. N. et al. (1982) Infect. Immun. 38:681. The Neutralization Dose (ND50) is typically 4-20 ng/mL in the presence of 1 ng/mL Recombinant Mouse IFN‑ gamma. | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Neutralization |
![]() click image to view larger | IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R1/CD119 Antibody.Recombinant Mouse IFN‑ gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IFN‑ gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IFN‑ gamma R1/CD119 Monoclonal Antibody (Catalog # MAB10262). The ND50 is typically 4-20 ng/mL. |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: IFN-gamma R1/CD119 | The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids. |
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References
Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563. | |
Long Name: | Interferon gamma Receptor 1 |
Entrez Gene IDs: | 3459 (Human); 15979 (Mouse) |
Alternate Names: | AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2 |
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Related Research Areas
B Cell Activation | ||
Cytokine and Growth Factor Receptors on VSMC | ||
Cytokines and Receptors in Allergy and Asthma | ||
Cytokines and Receptors in Angiogenesis | ||
Cytokines and Receptors in Tfh Cell Differentiation and Function | ||
IL-10/Interferon Family | ||
Macrophage Activation Markers | ||
Receptors in the Jak/STAT Pathway | ||
Regulatory T Cells (Tregs) | ||
T Cell Cytokine Signaling | ||
Th1 Cells | ||
Th2 Cells | ||
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Related Product & Information
Long Name | Interferon gamma Receptor 1 |
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Entrez Gene IDs | 3459 (Human); 15979 (Mouse) |
Background | IFN-gamma R1/CD119 |
background_content | Background: IFN-gamma R1/CD119 The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids. |
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Citations
- CD40L-expressing CD8 T cells prime CD8alpha(+) DC for IL-12p70 production.
Authors: Wong KL, Lew FC, MacAry PA, Kemeny DM
Eur. J. Immunol., 2008;38(8):2251-62.
Species: Mouse
Sample Type: Whole Cells
Application: Neut
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