抗CD16抗体(Anti-CD16, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma  RIIIAla31-Thr215Accession # Q5D5J5
Source	Monoclonal Rat IgG2A Clone # 275003
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Fcrl3/CD16-2, rmFc gamma RIA or rhFc gamma RIIA is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of Fc gamma RIII (CD16) in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Fc gamma RIII (CD16) Monoclonal Antibody (Catalog # MAB19601, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

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Related Product & Information

Background

Fc gamma RIII (CD16)

background_content

Background: Fc gamma RIII (CD16) Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8‑10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6‑10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

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Citations

1. C5aR activation in the absence of C5a - a new disease mechanism of Autoimmune Hemolytic Anemia in mice
   Authors: SN Syed, E Rau, M Ziegelmann, G Sogkas, B Brüne, RE Schmidt
   Eur. J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. DC subset-specific induction of T cell responses upon antigen uptake via Fc? receptors in vivo
   Authors: CH Lehmann, A Baranska, GF Heidkamp, L Heger, K Neubert, JJ Lühr, A Hoffmann, KC Reimer, C Brückner, S Beck, M Seeling, M Kie beta ling, D Soulat, AB Krug, JV Ravetch, JH Leusen, F Nimmerjahn, D Dudziak
   J. Exp. Med., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


3. IgG subclasses determine pathways of anaphylaxis in mice
   Authors: H Beutier, CM Gillis, B Iannascoli, O Godon, P England, R Sibilano, LL Reber, SJ Galli, MS Cragg, N Van Rooije, DA Mancardi, P Bruhns, F Jönsson
   J. Allergy Clin. Immunol., 2017;139(1):269-280.e7.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Rapid desensitization of mice with anti-FcgammaRIIb/FcgammaRIII mAb safely prevents IgG-mediated anaphylaxis.
   Authors: Khodoun M, Kucuk Z, Strait R, Krishnamurthy D, Janek K, Clay C, Morris S, Finkelman F
   J Allergy Clin Immunol, 2013;132(6):1375-87.
   Species: Mouse
   Sample Type: In Vivo
   Application: Functional Assay


5. Cutting edge: FcgammaRIII (CD16) and FcgammaRI (CD64) are responsible for anti-glycoprotein 75 monoclonal antibody TA99 therapy for experimental metastatic B16 melanoma.
   Authors: Albanesi M, Mancardi D, Macdonald L, Iannascoli B, Zitvogel L, Murphy A, Daeron M, Leusen J, Bruhns P
   J Immunol, 2012;189(12):5513-7.
   Species: Mouse
   Sample Type: In Vivo
   Application: Bioassay


6. Facilitated antigen uptake and timed exposure to TLR ligands dictate the antigen-presenting potential of plasmacytoid DCs.
   Authors: Kool M, Geurtsvankessel C, Muskens F, Madeira FB, van Nimwegen M, Kuipers H, Thielemans K, Hoogsteden HC, Hammad H, Lambrecht BN
   J. Leukoc. Biol., 2011;90(6):1177-90.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. beta5 integrin is the major contributor to the alphaVintegrin-mediated blockade of HIV-1 replication.
   Authors: Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Este JA
   J. Immunol., 2011;186(1):464-70.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


8. Mouse and human neutrophils induce anaphylaxis.
   Authors: Jonsson F, Mancardi DA, Kita Y, Karasuyama H, Iannascoli B, Van Rooijen N, Shimizu T, Daeron M, Bruhns P
   J. Clin. Invest., 2011;121(4):1484-96.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. Both FcgammaRIV and FcgammaRIII are essential receptors mediating type II and type III autoimmune responses via FcRgamma-LAT-dependent generation of C5a.
   Authors: Syed SN, Konrad S, Wiege K, Nieswandt B, Nimmerjahn F, Schmidt RE, Gessner JE
   Eur. J. Immunol., 2009;39(12):3343-56.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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