抗Mouse IL-12/IL-23 p40抗体(Anti-Mouse IL-12/IL-23 p40 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Recombinant mouse IL-12 p70 heterodimer
Source	Monoclonal Rat IgG2A Clone # C17.8
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects the mouse IL-12/IL-23 p40 subunit. Polymorphisms exist in the mouse p40 sequences [Ymer, S. et al. (2002) Genes and Immunity 3:151.].

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IL-12/IL-23 p40 Homodimer (Catalog # 499-ML) under non-reducing conditions only
Mouse IL-12/IL-23 p40 Nonallele-specific Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑12/IL‑23 p40 Antibody (Catalog #MAB4991 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑12/IL‑23 p40 Biotinylated Antibody (Catalog #BAF499 )
ELISA Standard		Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein (Catalog #499-ML )
Neutralization	Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated mouse splenocytes. The Neutralization Dose (ND50) is typically 0.04-0.3 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑12.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12/IL‑23 p40 Antibody.Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND50 is typically 0.04-0.3 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12/IL-23 p40	Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells. IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells. The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.
Long Name:	Interleukin 12/Interleukin 23 p40
Entrez Gene IDs:	3593 (Human); 16160 (Mouse); 64546 (Rat); 403976 (Canine); 493741 (Feline)
Alternate Names:	CLMF2; IL12 p40; IL-12 p40; IL12B; IL-12B; NKSF2

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Related Research Areas

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

gamma delta T Cells

IL-12 Family

Th1 Cells

Th17 Cells

IFN-?-Expressing Th17 Cells Are Required for Development of Severe Ocular Surface Autoimmunity Authors: Y Chen, SK Chauhan, C Shao, M Omoto, T Inomata, R Dana J. Immunol., 2017;0(0):. Species: Mouse Sample Type: In Vivo Application: In Vivo

Neutralization

Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12/IL‑23 p40 Antibody. Recombinant Mouse IL‑12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND50 is typically0.04-0.3 µg/mL.

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Related Product & Information

Long Name	Interleukin 12/Interleukin 23 p40
Entrez Gene IDs	3593 (Human); 16160 (Mouse); 64546 (Rat); 403976 (Canine); 493741 (Feline)
Background	IL-12/IL-23 p40
background_content	Background: IL-12/IL-23 p40 Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells. IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells. The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

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Citations

1. IFN-?-Expressing Th17 Cells Are Required for Development of Severe Ocular Surface Autoimmunity
   Authors: Y Chen, SK Chauhan, C Shao, M Omoto, T Inomata, R Dana
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In Vivo


2. M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
   Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
   Am J Pathol, 2015;185(2):420-31.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


3. Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens.
   Authors: DePaolo RW, Abadie V, Tang F
   Nature, 2011;471(7337):220-4.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. The pro-Th2 cytokine IL-33 directly interacts with invariant NKT and NK cells to induce IFN-gamma production.
   Authors: Bourgeois E, Van LP, Samson M, Diem S, Barra A, Roga S, Gombert JM, Schneider E, Dy M, Gourdy P, Girard JP, Herbelin A
   Eur. J. Immunol., 2009;39(4):1046-55.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
   Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
   Cancer Res., 2008;68(23):9754-62.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
   Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
   J Ethnopharmacol, 2006;106(3):418-24.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
   Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
   Blood, 2004;104(5):1404-10.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


8. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.
   Authors: Oppmann, B, Lesley, R, Blom, B, Timans, J C, Xu, Y, Hunte, B, Vega, F, Yu, N, Wang, J, Singh, K, Zonin, F, Vaisberg, E, Churakova, T, Liu, M, Gorman, D, Wagner, J, Zurawski, S, Liu, Y, Abrams, J S, Moore, K W, Rennick, D, de Waal-Malefyt, R, Hannum, C, Bazan, J F, Kastelein, R A
   Immunity, 2000;13(5):715-25.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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